Universal immune peroxidase anti mouse

Anti-ORP3 rabbit antibody (1:2000 for WB and 1:400 for IF, A304-557A, Bethyl Laboratories, Texas, USA). Anti-ORP3 mouse antibody (1:400 for IHC and 1:2000 for WB, sc398326, Santa Cruz, California, USA). Anti-Cortactin mouse antibody (1:500 for IF, sc-55579, Santa Cruz, California, USA). Anti-α-tubulin mouse antibody (1:2000 for WB, T5168, Sigma-Aldrich, St. Louis, USA). Anti-γ-tubulin mouse antibody (1:2000 for WB and 1:500 for IF, T5326, Sigma-Aldrich, St. Louis, USA). Anti-γ-tubulin rabbit antibody (1:500 for IF, T5192, Sigma-Aldrich, St. Louis, USA). Anti-β-actin mouse antibody (1:10,000 for WB, A1978, Sigma-Aldrich, St. Louis, USA). Secondary antibodies, HRP anti-Mouse antibody (1:5000 for WB, 7076S, Cell Signaling Technology, Danvers, USA) and HRP anti-Rabbit (1:2000 for WB, 7074S, Cell Signaling Technology, Danvers, USA). One drop of Universal immune-peroxidase anti-Mouse (Nichirei Bioscience, Tokyo, JP) per piece of tissue was used in IHC. Phalloidin-iFluor 488 Reagent (1:1000 for IF, ab176753, Abcam, Cambridge, UK). Phalloidin-iFluor647 reagent (1: 1000 for IF, ab176759, Abcam, Cambridge, UK). Anti-Rabbit conjugated with Alexa Fluor488 antibody (1:1000 for IF, A11008, ThermoFisher Scientific, Waltham, USA), and anti-Mouse conjugated with Cy5 antibody (1:200 for IF, 115–605-006, ThermoFisher Scientific, Waltham, USA).

Anti-ORP3 rabbit antibody (1:2000 for WB and 1:400 for IF, A304-557A, Bethyl Laboratories, Texas, USA). Anti-ORP3 mouse antibody (1:400 for IHC and 1:2000 for WB, sc398326, Santa Cruz, California, USA). Anti-Cortactin mouse antibody (1:500 for IF, sc-55579, Santa Cruz, California, USA). Anti-α-tubulin mouse antibody (1:2000 for WB, T5168, Sigma-Aldrich, St. Louis, USA). Anti-γ-tubulin mouse antibody (1:2000 for WB and 1:500 for IF, T5326, Sigma-Aldrich, St. Louis, USA). Anti-γ-tubulin rabbit antibody (1:500 for IF, T5192, Sigma-Aldrich, St. Louis, USA). Anti-β-actin mouse antibody (1:10,000 for WB, A1978, Sigma-Aldrich, St. Louis, USA). Secondary antibodies, HRP anti-Mouse antibody (1:5000 for WB, 7076S, Cell Signaling Technology, Danvers, USA) and HRP anti-Rabbit (1:2000 for WB, 7074S, Cell Signaling Technology, Danvers, USA). One drop of Universal immune-peroxidase anti-Mouse (Nichirei Bioscience, Tokyo, JP) per piece of tissue was used in IHC. Phalloidin-iFluor 488 Reagent (1:1000 for IF, ab176753, Abcam, Cambridge, UK). Phalloidin-iFluor647 reagent (1: 1000 for IF, ab176759, Abcam, Cambridge, UK). Anti-Rabbit conjugated with Alexa Fluor488 antibody (1:1000 for IF, A11008, ThermoFisher Scientific, Waltham, USA), and anti-Mouse conjugated with Cy5 antibody (1:200 for IF, 115–605-006, ThermoFisher Scientific, Waltham, USA).