Fdss7000 system

Manufactured by Hamamatsu Photonics

Sourced in Japan

The FDSS7000 system is a fluorescence detection and analysis instrument developed by Hamamatsu Photonics. It is designed to measure and analyze fluorescence signals in a variety of experimental settings. The system features a sensitive detector, advanced optics, and sophisticated software for data acquisition and processing. The core function of the FDSS7000 is to provide researchers with a reliable and versatile tool for quantifying fluorescent signals in their experiments.

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Lab products found in correlation

Flipr calcium 5, by Molecular Devices (1 mentions) Hank s balanced salt solution, by Thermo Fisher Scientific (1 mentions) Earlytox cardiotoxicity kit, by Molecular Devices (1 mentions)

2 protocols using fdss7000 system

Calcium Imaging of Neuronal Cells

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Neurons were incubated for 1h with the fluorescent calcium indicator FLIPR Calcium 5 (Molecular Devices R8186) diluted 3x more than providers recommendation in Hanks balanced salt solution (ThermoFisher Scientific, 14175) with 20 mM Hepes and 1.26 mM CaCl2. Image based measurements of cytosolic calcium kinetics were conducted at 37°C and with a 1 Hz sampling frequency (excitation and emission wavelengths of 480, and 540 nm, respectively) using the FDSS7000 system (Hamamatsu, Japan). The instrument readout is the total fluorescent intensity per well and data are reported as ratio to the first sampled data point.

Vajhøj C., Schmid B., Alik A., Melki R., Fog K., Holst B, & Stummann T.C. (2021). Establishment of a human induced pluripotent stem cell neuronal model for identification of modulators of A53T α-synuclein levels and aggregation. PLoS ONE, 16(12), e0261536.

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High-throughput cardiotoxicity assessment

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Following pre-culture, hiPSC-CMs cells were seeded in black 384-well
plates with a transparent bottom (Corning) in minimal media and treated with
drugs at a range of doses (0.1 to 10 μM) and times (6 hours and 1, 2,
3 and 6 days) in 3 biological replicates. At each time point, cells were
stained for 30 min with an EarlyTox Cardiotoxicity Kit (Molecular Devices),
which contains a less toxic modified calcium dye than traditional dyes, in a
cell culture incubator (5% CO₂ and 37°C). Fluorescence
intensity was recorded using a FDSS 7000 System (Hamamatsu) at 37°C.
Beats per minute were calculated using the waveform add-on software for the
FDSS 7000 device (Hamamatsu).

Wang H., Sheehan R.P., Palmer A.C., Everley R.A., Boswell S.A., Ron-Harel N., Ringel A.E., Holton K.M., Jacobson C.A., Erickson A.R., Maliszewski L., Haigis M.C, & Sorger P.K. (2019). Adaptation of human iPSC-derived cardiomyocytes to tyrosine kinase inhibitors reduces acute cardiotoxicity via metabolic reprogramming. Cell systems, 8(5), 412-426.e7.

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