Total bile acids (3α-hydroxy bile acids) concentration was measured in freeze-dried faeces [42 (link)] using Chromabond C18 ec (3 mL/200 mg, Macherey-Nagel, Düren, Germany) and in liver. Samples were analysed using the enzymatic bile acid assay from Diazyme Laboratories, Inc. on the Cobas c111 system (Roche). Liver protein was measured with the Bradford dye-binding method [43 (link)] using Protein Assay Dye Reagent (Bio-Rad Laboratories, Munich, Germany) with bovine serum albumin (Bio-Rad Protein Assay Standard II, Bio-Rad Laboratories, Hercules, CA, USA) as standard. Concentration of total bile acids in liver is calculated relative to protein concentration.
Protein assay standard 2
Manufactured by Bio-Rad
Sourced in Germany
The Bio-Rad Protein Assay Standard II is a reference standard for use in protein quantification assays. It provides a range of protein concentrations to generate a standard curve for the determination of unknown protein samples.
Automatically generated - may contain errors
5 protocols using protein assay standard 2
1
Quantification of Bile Acids in Feces and Liver
2
Protein Concentration Measurement in Biofluids
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The protein concentration in serum samples and peritoneal fluid was measured by Bio-Rad Protein Assay (Bio-Rad Laboratories, Hercules, CA, USA) and Bio-Rad Protein Assay Standard II (Bio-Rad Laboratories, Hercules, CA, USA).
3
Quantifying Total Protein in Biological Samples
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The total protein concentration in plasma samples, PF and urine was measured with Bio-Rad protein Assay (Bio-Rad Laboratories, Hercules, CA) and Bio-Rad protein Assay Standard II (Bio-Rad Laboratories, Hercules, CA).
4
Quantifying Liver Enzymes Using ELISA
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HMG-CoA reductase and LDL receptors were measured in liver using the ELISA kits LS-F15758 and LS-F11934 from LifeSpan BioSciences, Inc. (Seattle, WA, USA). Liver protein was quantified with the Bradford dye-binding method [48 (link)] using protein assay dye reagent (Bio-Rad Laboratories, Munich, Germany) with bovine serum albumin (Bio-Rad Protein Assay Standard II, Bio-Rad Laboratories, Hercules, CA, USA) as the standard. Concentrations of HMG-CoA reductase and LDL receptors in liver are presented relative to protein.
5
Quantification of Kidney TIM-1 Levels
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Kidneys were homogenised in TRIS-buffer (pH 7•8). TIM-1 in kidney homogenate was measured with the Rat TIM-1/KIM-1/ HAVCR Quantikine ® ELISA kit. Kidney protein content was measured with the Bradford dye-binding method (51) using Protein Assay Dye Reagent (Bio-Rad Laboratories) with bovine serum albumin (Bio-Rad Protein Assay Standard II, Bio-Rad Laboratories) as standard.
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