Goat anti pdgfr β

Manufactured by R&D Systems

Goat anti-PDGFR-β is a laboratory reagent used for the detection and analysis of the platelet-derived growth factor receptor beta (PDGFR-β) protein. It is a polyclonal antibody produced in goats that binds to PDGFR-β, a cell surface receptor involved in various cellular processes.

Automatically generated - may contain errors

Lab products found in correlation

Alexa fluor 647 phalloidin, by Thermo Fisher Scientific (1 mentions) Alexa fluor 555 phalloidin, by Thermo Fisher Scientific (1 mentions) Gpr124, by Abcam (1 mentions) Mouse anti paxillin, by Thermo Fisher Scientific (1 mentions) Mouse anti vinculin, by Abcam (1 mentions) Vectashield mounting medium, by Vector Laboratories (1 mentions) Las 3000 imaging system, by Fujifilm (1 mentions) Mouse anti gapdh, by Santa Cruz Biotechnology (1 mentions) Protease inhibitors mixture, by Merck Group (1 mentions) Bradford method, by Bio-Rad (1 mentions) Nitrocellulose membrane, by Bio-Rad (1 mentions) Non fat milk, by Bio-Rad (1 mentions) Rabbit anti ng2, by Merck Group (1 mentions) Sp8 laser confocal microscope, by Leica (1 mentions) Mouse anti cc1, by Abcam (1 mentions) Rabbit anti olig2, by Merck Group (1 mentions) Mouse anti o4, by Merck Group (1 mentions) Rnascope multiplex fluorescent reagent kit v2, by Advanced Cell Diagnostics (1 mentions) Rabbit anti iba1, by Abcam (1 mentions) Fv1000, by Leica (1 mentions)

Close spelling variants of this product

Goat biotinylated anti-PDGFR-β (2 citations)

3 protocols using goat anti pdgfr β

Immunostaining of Focal Adhesions

Check if the same lab product or an alternative is used in the 5 most similar protocols

Briefly, cells were fixed in 4% PFA as previously reported 31 (link). The cells were incubated with 0.1% Triton X-100 in PBS for 10 min at RT and for another hour in PBS containing 3% bovine serum albumin (BSA). The cells were incubated with primary antibodies, followed by labeling with corresponding fluorescent secondary antibodies. After staining with DAPI, cells were then rinsed with PBS and mounted onto glass slides with Vectashield mounting medium (Vector Laboratories). Images were captured using a NikonA1R confocal microscope.
The following primary antibodies were used: rabbit anti-GPCR GPR124 (GPR124, 1:300, Abcam, ab67820), rabbit anti-GPR124 (TEM5, 1:500, Invitrogen, PA5-20442), mouse anti-Vinculin (1:300, Abcam, ab18058), mouse anti-Paxillin (1:300, Thermofisher, MA5-13356), Alexa Fluor 647 Phalloidin (1:40, Invitrogen, A22287), Alexa Fluor 555 Phalloidin (1:40, Invitrogen, A34055), mouse anti-Myc-tag (1:2,000, Medical & biological laboratories, M192-3), goat anti-PDGFR-β (1:300, R&D systems, AF1042), rabbit anti-Partitioning-defective 3 (PAR-3, 1:500, Millipore, 07-330).
Isolated focal adhesions were stained with the following antibodies: rabbit anti- GPR124 (1:300, Abcam, ab67820), mouse anti-Vinculin (1:300, Abcam, ab18058), mouse anti-Paxillin (1:300, Thermofisher, MA5-13356).

Chen D.Y., Sun N.H., Lu Y.P., Hong L.J., Cui T.T., Wang C.K., Chen X.H., Wang S.S., Feng L.L., Shi W.X., Fukunaga K., Chen Z., Lu Y.M, & Han F. (2019). GPR124 facilitates pericyte polarization and migration by regulating the formation of filopodia during ischemic injury. Theranostics, 9(20), 5937-5955.

+ Open protocol

+ Expand

PDGFR-β Expression in Dental Stem Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Both wild type and PDGFR-β-KD DPSCs were washed with phosphate buffered saline (PBS) and placed in a single tube containing radioimmunoprecipitation assay solution (Sigma-Aldrich) and protease inhibitors mixture (Sigma-Aldrich). Constructs were homogenized, and the total protein content was determined using the Bradford method (Bio-Rad). Proteins were separated on a 4 to 12% SDS-PAGE (sodium dodecyl sulphate–polyacrylamide gel electrophoresis) gel (NuPage, Life Technologies) and transferred to a nitrocellulose membrane (Bio-Rad). After blocking with 5% nonfat milk (Bio-Rad), the membranes were immunoblotted (overnight, at 4 °C) with primary antibodies: 1:2,000 goat anti–PDGFR-β (R&D) and 1:1,000 mouse anti-GAPDH (Santa Cruz) diluted in blocking buffer. The bound antibodies were detected following incubation with horseradish peroxidas–conjugated anti-mouse/goat IgG (GE Healthcare Life Sciences), followed by exposure to the enhanced chemiluminescence Western blotting system (ECL; Amersham Biosciences). Images were acquired with the LAS-3000 imaging system (FujiFilm). Dense cytometry analysis was performed using multigauge software (Bioz). Data are presented relative to GAPDH.

Landau S., Newman A., Edri S., Michael I., Ben-Shaul S., Shandalov Y., Ben-Arye T., Kaur P., Zheng M.H, & Levenberg S. (2021). Investigating lymphangiogenesis in vitro and in vivo using engineered human lymphatic vessel networks. Proceedings of the National Academy of Sciences of the United States of America, 118(31), e2101931118.

+ Open protocol

+ Expand

In Situ Hybridization and Immunofluorescence of Mouse Hrh2

Check if the same lab product or an alternative is used in the 5 most similar protocols

Frozen brain slices with 14-µm thickness mounted on adhesion microscope slides (CITOGLAS) were subjected to in situ hybridization. The mouse Hrh2 probe and RNAscope 2.5 HD Detection Reagent Kit-Red/RNAscope Multiplex Fluorescent Reagent Kit v2 (Advanced Cell Diagnostics) were used for Hrh2 expression in OL lineages, microglia, astrocytes, or pericytes, together with immunostaining of NG2, O4, CNP, CC-1, Olig2, Iba-1, GFAP, PDGFRβ, or GFP. RNAscope Multiplex Fluorescent Reagent Kit v2 (Advanced Cell Diagnostics) was used for duplex hybridization by combining the Hrh2 probe C1 with a GFP probe C2 for validation of Hrh2 reexpression. For combined in situ hybridization and immunofluorescence analyses, the slices were then incubated with rabbit anti-NG2 (1:300, Millipore), mouse anti-O4 (1:150, Millipore), rabbit anti-CNP (1:400, Abcam), mouse anti-CC-1 (1:400, Abcam), rabbit anti-Olig2 (1:500, Millipore), rabbit anti-Iba-1 (1:200, Abcam), rabbit anti-GFAP (1:400, Lianke), goat anti-PDGFRβ (1:20, R&D Systems), or chicken anti-GFP (1:400, Abcam). Fluorescent images were taken using an Olympus FV1000 or Leica SP8 laser confocal microscope.

Jiang L., Cheng L., Chen H., Dai H., An D., Ma Q., Zheng Y., Zhang X., Hu W, & Chen Z. (2020). Histamine H2 receptor negatively regulates oligodendrocyte differentiation in neonatal hypoxic-ischemic white matter injury. The Journal of Experimental Medicine, 218(1), e20191365.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!