Sulforaphane (SF), menadione, digitonin, As(III), and flavin adenine dinucleotide were purchased from Sigma-Aldrich (MO, USA). Cycloheximide (CHX), 4′, 6-diamidino-2-phenylindole (DAPI), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT), bromophenol blue, glycerol, and bovine serum albumin (BSA) were obtained from Genview (TX, USA). Glucose-6-phosphate dehydrogenase and nicotinamide adenine dinucleoide phosphate (NADP) were purchased from Regal (Shanghai, China). β-Mercaptoethanol was obtained from Dingguo (Beijing, China). Eagle's minimal essential medium (MEM), and RPMI1640 were acquired from Gibco (CA, USA). Fetal bovine serum (FBS) was purchased from Gemini Bio-product (CA, USA). Glucose-6-phosphate and L -glutamine were obtained from Solarbio (Beijing, China).
Glucose 6 phosphate (g6p)
Manufactured by Solarbio
Sourced in China
Glucose-6-phosphate is a key intermediate in the metabolism of carbohydrates. It is an important substrate in the glycolysis pathway and the pentose phosphate pathway. Glucose-6-phosphate serves as a precursor for the synthesis of other important biomolecules.
Automatically generated - may contain errors
Lab products found in correlation
Digitonin, by Merck Group (2 mentions)
Glycerol, by Genview (2 mentions)
Eagle s minimal essential medium, by Thermo Fisher Scientific (2 mentions)
As 3, by Merck Group (2 mentions)
β mercaptoethanol, by Dingguo (2 mentions)
Bovine serum albumin (bsa), by Genview (2 mentions)
Rpmi 1640, by Thermo Fisher Scientific (2 mentions)
Flavin adenine dinucleotide, by Merck Group (2 mentions)
L glutamine, by Solarbio (2 mentions)
Fetal bovine serum (fbs), by Gemini Bio (2 mentions)
Haemin, by Merck Group (1 mentions)
Glucose 6 phosphatase dehydrogenase, by Merck Group (1 mentions)
Malic acid, by Solarbio (1 mentions)
Hepes, by Solarbio (1 mentions)
Nad p, by Solarbio (1 mentions)
Mgcl2, by Sinopharm (1 mentions)
Didox, by MedChemExpress (1 mentions)
Naphthylethylenediamine, by Sinopharm (1 mentions)
Sulfanilamide, by Sinopharm (1 mentions)
4 protocols using glucose 6 phosphate (g6p)
1
Evaluating Cellular Responses to Oxidative Stress
2
Measuring HO-1 Activity in Cell Lysates
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HO-1 activity was measured as previously described with minor modification [23] (link). Cell lysates were added to the reaction mixture containing 30 μM haemin (Sigma–Aldrich), 2 mg/ml of rat liver cytosol, 1 mM Mgcl2, 3 units of glucose-6-phosphatase dehydrogenase (Sigma–Aldrich), 1 mM glucose-6-phosphate (Solarbio Science & Technology, Beijing, China), 2 mM reduced nicotinamide adenine dinucleotide phosphate (Beyotime Institute of Biotechnology, Jiangsu, China) and 0.1 M potassium phosphate buffer. The reaction was conducted at 37°C in the dark for 30 min and terminated by the addition of 1 ml of chloroform. The concentration of bilirubin was determined as the difference between the concentrations measured at 464 nm and 530 nm. The results were expressed as picomoles of bilirubin per milligram of protein.
3
Enzymatic Activities in Tomato Roots
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The root activity of tomato was determined with the triphenyltetrazolium chloride (TTC) method as previously described [45 (link)].
The activities of 6PGDH, G6PDH, NADP-IDCH and NADP-ME were measured by spectrophotometry, recording the reduction of NADP at 340 nm [46 (link), 47 (link)]. The experiment was conducted at 25 °C, and the reaction system volume was 1 ml, including 50 mM HEPES (pH 7.6, Solarbio, Beijing, China), 2 mM MgCl2 (Sinopharm Chemical Reagent, Shanghai, China), 0.8 mM NADP (Solarbio, Beijing, China) and plant samples. The reaction was initiated by adding 5 mM 6-phosphate gluconate (Aladdin, Shanghai, China), 5 mM glucose 6-phosphate (Solarbio, Beijing, China), 10 mM 2R,3S-isocitrate (Aladdin, Shanghai, China) and 10 mM malic acid (Solarbio, Beijing, China), respectively.
The activities of 6PGDH, G6PDH, NADP-IDCH and NADP-ME were measured by spectrophotometry, recording the reduction of NADP at 340 nm [46 (link), 47 (link)]. The experiment was conducted at 25 °C, and the reaction system volume was 1 ml, including 50 mM HEPES (pH 7.6, Solarbio, Beijing, China), 2 mM MgCl2 (Sinopharm Chemical Reagent, Shanghai, China), 0.8 mM NADP (Solarbio, Beijing, China) and plant samples. The reaction was initiated by adding 5 mM 6-phosphate gluconate (Aladdin, Shanghai, China), 5 mM glucose 6-phosphate (Solarbio, Beijing, China), 10 mM 2R,3S-isocitrate (Aladdin, Shanghai, China) and 10 mM malic acid (Solarbio, Beijing, China), respectively.
4
Biochemicals and Reagents for Cell Experiments
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Sulforaphane (SF), menadione, digitonin, As(III), and flavin adenine dinucleotide were purchased from Sigma-Aldrich (MO, USA). Didox was purchased from MedChemExpress (Monmouth Junction, NJ, USA). Cycloheximide (CHX), 4′,6-diamidino-2-phenylindole (DAPI), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT), bromophenol blue, glycerol, and bovine serum albumin (BSA) were purchased from Genview (TX, USA). β-Mercaptoethanol was obtained from Dingguo (Beijing, China). Eagle's minimal essential medium (MEM) and RPMI 1640 were acquired from Gibco (CA, USA). Fetal bovine serum (FBS) was purchased from Gemini Bio Products (CA, USA). Glucose-6-phosphate and L -glutamine were obtained from Solarbio (Beijing, China). Glucose-6-phosphate dehydrogenase and nicotinamide adenine dinucleotide phosphate (NADP) were obtained from Regal (Shanghai, China). Solvents used for extraction and isolation were of analytical grade and obtained from Tianjin Fuyu Fine Chemical Company (Tianjin, China). Naphthylethylenediamine and sulfanilamide were obtained from Sinopharm Chemical Reagent Co. Ltd. (China).
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