Hplc grade methylene chloride

Manufactured by Merck Group

Sourced in United States

HPLC grade methylene chloride is a high-purity solvent commonly used in analytical and laboratory applications. It is a colorless, volatile liquid with a characteristic odor. The product meets the specification requirements for use in high-performance liquid chromatography (HPLC) and other sensitive analytical techniques.

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HPLC grade (319 citations) Methylene chloride (91 citations)

4 protocols using hplc grade methylene chloride

Analytical Methods for PAH Determination

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Chemical reagents for analytical methods such as HPLC grade acetonitrile, methanol, hexane and acetone were from Merck (Darmstadt, Germany). HPLC grade methylene chloride came from Sigma Aldrich (Oakville, ON, USA). Polycyclic Aromatic Hydrocarbon (PAHs) were purchased as stock solution of 100 mg/L in cyclohexane from Aldrich (Steinheim, Germany) at 98% purity.

Saccà M.L., Ferrero V.E., Loos R., Di Lenola M., Tavazzi S., Grenni P., Ademollo N., Patrolecco L., Huggett J., Caracciolo A.B, & Lettieri T. (2019). Chemical mixtures and fluorescence in situ hybridization analysis of natural microbial community in the Tiber river. The Science of the Total Environment, 673, 7-19.

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Fluorene and Hydroxy-Fluorene Standards

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Standards of fluorene (Flu, ≥ 99% purity), 2-hydroxyfluorene (2OH-Flu, ≥ 98% purity), 3hydroxyfluorene (3OH-Flu, ≥ 98% purity), 9-hydroxyfluorene (9OH-Flu, ≥ 96% purity), were purchased from Sigma-Aldrich (Saint-Quentin Fallavier, France). HPLC-grade methylene chloride (DCM), acetonitrile (ACN), methanol (MeOH), pyridine, dimethylsulfoxide (DMSO) and the silylation reagent: N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA) with 1% of trimethylchlorosilane (TMCS) were also purchased from Sigma-Aldrich (France). Ultrapure water used for photolysis experiments and SPE extraction was obtained by purification of drinking water using a Millipore Milli-Q device (resistivity < 18 MΩ.cm ; DOC < 0.1 mg.L -1 ; Millipore, CA, USA). A stock solution of individual compounds: Flu, 2OH-Flu, 3OH-Flu and 9OH-Flu, each one at 10 µg.mL -1 , was prepared in amber vials by dissolving the appropriate amount of each chemical in anhydrous methylene chloride. It was used for the development of the GC-MS analytical method. All the standard chemicals were prepared in DMSO for bioassay testing.

Kinani S., Souissi Y., Kinani A., Vujović S., Aït-Aïssa S, & Bouchonnet S. (2016). Photodegradation of fluorene in aqueous solution: Identification and biological activity testing of degradation products. Journal of chromatography. A, 1442.

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Quantitative Analysis of Sulfatase Activity

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Sulfatase from Helix pomatia, sinigrin hydrate, l-ascorbic acid (AA), trichloroacetic acid (TCA), dl-dithiothreitol (DTT), N-ethylmaleimide (NEM), 2,2′-bipyridyl, iron (III) chloride (FeCl₃), 3-O-CQA (3-O-caffeoylquinic acid), 5-O-CQA (5-O-caffeoylquinic acid), butyl-isothiocyanate, caffeic acid (CA), 1,2-benzenedithiol, ß-mercaptoethanol (HPLC grade), methylene chloride (HPLC grade), sephadex A-25, sephadex C-25, acetonitrile (HPLC grade), methanol (HPLC grade), sulforaphane, sodium acetate, potassium phosphate, and orthophosphoric acid (H₃PO₄) were obtained from Sigma Chemical Co. (St. Louis, MO, USA). Desulfoglucoraphanin was obtained from Santa Cruz Biotechnology (Dallas, TX, USA).

Torres-Contreras A.M., Nair V., Cisneros-Zevallos L, & Jacobo-Velázquez D.A. (2017). Stability of Bioactive Compounds in Broccoli as Affected by Cutting Styles and Storage Time. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 22(4), 636.

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Formulation and Characterization of Quercetin-Loaded Nanoparticles

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Quercetin, cholesterol, Brij 35 (polyoxyethylene lauryl ether), Tween 80 (polyoxyethylene sorbitan monooleate), sodium selenite, ascorbic acid, methylene chloride (HPLC grade), absolute ethanol (HPLC grade), and glacial acetic acid (HPLC grade) were procured from Sigma-Aldrich (St. Louis, MO, USA). Dialysis bags (Mol. Wt. cut off = 12,000 Da) were acquired from SERVA Electrophoresis GmbH (Heidelberg, Germany). Oleic acid, polyethylene glycol 400, potassium dihydrogen orthophosphate, potassium chloride, disodium hydrogen orthophosphate, and sodium chloride were provided by El-Nasr pharmaceutical chemical company (Cairo, Egypt). Technetium-99m was eluted as ^99mTcO₄⁻ from ⁹⁹Mo/^99mTc generator, Radioisotope Production Facility, Cairo, Egypt. All other chemicals and solvents were of analytical grade and were used as received without any further modifications.

Aboud H.M., Hussein A.K., Zayan A.Z., Makram T.S., Sarhan M.O, & El-Sharawy D.M. (2022). Tailoring of Selenium-Plated Novasomes for Fine-Tuning Pharmacokinetic and Tumor Uptake of Quercetin: In Vitro Optimization and In Vivo Radiobiodistribution Assessment in Ehrlich Tumor-Bearing Mice. Pharmaceutics, 14(4), 875.

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