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Dnase digest

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DNase digest is a laboratory tool used to degrade DNA molecules. It functions by catalyzing the hydrolytic cleavage of phosphodiester bonds in DNA, effectively breaking down the DNA strands.

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Lab products found in correlation

Amv rt, by Promega (1 mentions) Trizol reagent, by Thermo Fisher Scientific (1 mentions) Random hexamer, by Cytiva (1 mentions) Gotaq qpcr master mix, by Promega (1 mentions)

Spelling variants of this product

DNase (479 citations) On-column DNase I digest (2 citations)

2 protocols using dnase digest

1

Quantitative Analysis of Gene Expression

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Total RNA of cultured cells and human cardiac biopsies was isolated using TRIzol reagent (Invitrogen) followed by DNase digest (Promega) and ethanol precipitation according to standard protocols. Reverse transcription reactions were carried out via AMV-RT (Promega) with random hexamers (Amersham Pharmacia Biotech). Quantitative real-time PCR measurements were performed using the GoTaq qPCR Master Mix (Promega) and the ABI PRISM 7900HT Sequence Detection System. Gene expression was calculated using the ΔCT method with normalization to the housekeeping gene HPRT or GAPDH. Primer sequences are given in Supplementary Table S3.
Cui H., Schlesinger J., Schoenhals S., Tönjes M., Dunkel I., Meierhofer D., Cano E., Schulz K., Berger M.F., Haack T., Abdelilah-Seyfried S., Bulyk M.L., Sauer S, & Sperling S.R. (2015). Phosphorylation of the chromatin remodeling factor DPF3a induces cardiac hypertrophy through releasing HEY repressors from DNA. Nucleic Acids Research, 44(6), 2538-2553.
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2

Isolation and Quantification of Liver RNA

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Total cellular RNA was isolated from liver tissue as described (Chomczynski and Sacchi, 1987) , including a DNase digest (Promega, D€ ubendorf, Switzerland). cDNA synthesis and qRT-PCR were carried out as described previously (Bo ¨hm et al., 2010) , using a Light Cycler reaction volume of 10ml.
Bachofner M., Speicher T., Bogorad R.L., Muzumdar S., Derrer C.P., Hürlimann F., Böhm F., Nanni P., Kockmann T., Kachaylo E., Meyer M., Padrissa-Altés S., Graf R., Anderson D.G., Koteliansky V., Auf dem Keller U, & Werner S. (2017). Large-Scale Quantitative Proteomics Identifies the Ubiquitin Ligase Nedd4-1 as an Essential Regulator of Liver Regeneration. Developmental cell, 42(6).
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