BM, peripheral blood (PB), and fetal liver (FL) single cell suspensions were prepared and flow cytometry was performed as described 23 (link), 24 (link), using an Accuri C6 (BD Biosciences) or DXP11 analyzer (Cytek, Fremont, CA), or the FACSAria sorter as well. Sorting was performed using a FACSAria sorter (BD Biosciences). The following antibodies were used: CD41-FITC, Sca-1-FITC, Ter119-PE, CD45-PE, CD150-PE, Sca-1-PE, and c-kit-APC, as well as biotinylated B220, Mac-1, Gr-1, Ter-119, CD3e [i.e., lineage markers for BM, or for FL (without Mac-1)]; CD45, CD31, Ter119 (i.e., lineage markers for non-hematopoietic tissues), followed by streptavidin-PerCPCy5.5, Sca-1-APCcy7, c-kit-BV711, CD150-PE, CD48-APC. All antibodies were from eBioscience (San Diego, CA) or BD Pharmingen.
Dxp11 analyzer
Manufactured by Cytek
The DXP11 analyzer is a flow cytometry instrument manufactured by Cytek. It is designed to analyze and quantify various cellular properties and populations within a sample. The DXP11 utilizes advanced optical and detection technologies to provide high-resolution data on parameters such as cell size, granularity, and fluorescent marker expression.
Automatically generated - may contain errors
Lab products found in correlation
Facsaria sorter, by BD (3 mentions)
Accuri c6, by BD (2 mentions)
Cd14 pe, by Thermo Fisher Scientific (1 mentions)
Accuri c6 analyzer, by BD (1 mentions)
Cd49b pe, by Thermo Fisher Scientific (1 mentions)
Cd29 apc, by Thermo Fisher Scientific (1 mentions)
C kit pe cy7, by Thermo Fisher Scientific (1 mentions)
Cd24 efluor450, by Thermo Fisher Scientific (1 mentions)
Cd24 efluor605, by Thermo Fisher Scientific (1 mentions)
3 protocols using dxp11 analyzer
1
Multiparametric Analysis of Hematopoietic Cells
2
Mammary Gland Cell Isolation and Flow Cytometry
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Thoracic and inguinal mammary glands were dissected from pubertal or adult virgin
female mice and cell suspensions were prepared as previously described (Shackleton et al., 2006 (link)). Flow cytometric
analysis was performed with a DXP11 analyzer (Cytek, Fremont, CA) or an Accuri C6
analyzer (BD Biosciences, San Jose, CA). FACS sorting was performed with a FACSAria
sorter (BD Biosciences). Data were analyzed with FlowJo (Tree Star, Ashland, OR) or
CFlow (BD Biosciences). Antibodies used for FACS were purchased from eBiosciences
(San Diego, Ca) and included CD24-eFluor450, CD24-eFluor605, CD29-APC, CD61-PE,
c-Kit-PE-CY7, CD14-PE, CD49b-PE, Sca1-APC and biotinylated CD31, CD45, and TER119
(i.e., lineage [Lin] markers), as well as Streptavidin-PerCP-CY5.5. We also used a
Sca1-APC-CY7 antibody purchased from BD biosciences (San Jose, CA).
female mice and cell suspensions were prepared as previously described (Shackleton et al., 2006 (link)). Flow cytometric
analysis was performed with a DXP11 analyzer (Cytek, Fremont, CA) or an Accuri C6
analyzer (BD Biosciences, San Jose, CA). FACS sorting was performed with a FACSAria
sorter (BD Biosciences). Data were analyzed with FlowJo (Tree Star, Ashland, OR) or
CFlow (BD Biosciences). Antibodies used for FACS were purchased from eBiosciences
(San Diego, Ca) and included CD24-eFluor450, CD24-eFluor605, CD29-APC, CD61-PE,
c-Kit-PE-CY7, CD14-PE, CD49b-PE, Sca1-APC and biotinylated CD31, CD45, and TER119
(i.e., lineage [Lin] markers), as well as Streptavidin-PerCP-CY5.5. We also used a
Sca1-APC-CY7 antibody purchased from BD biosciences (San Jose, CA).
3
Multiparameter Flow Cytometry of Hematopoietic Cells
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BM, peripheral blood (PB), and FL single cell suspensions were prepared and flow cytometry was performed as described17 (link),48 (link) using an Accuri C6 (BD Biosciences) analyzer, or DXP11 analyzer (Cytek, Fremont, CA) or the FACSAria sorter (BD Biosciences). Sorting was performed using a FACSAria sorter. The following antibodies were used: CD3e-biotin, B220-biotin, Mac1-biotin, Gr1-biotin, Ter119-biotin were used as lineage for adult BM; CD3e-biotin, B220-biotin, Gr1-biotin, Ter119-biotin were used as lineage for FLs. Streptavidin-eFluo700, Sca1-APCcy7, c-kit-PerCPcy5.5, CD150-PE, and CD48-APC were used for HSPCs. CD3e-PE, B220-APC, Mac1-PEcy7, Mac1-PE, Gr1-APCcy7, Ter119-biotin, Ter119-APC, and Streptavidin-PerCP, were used to study mature lineages. CD45.1-PEcy7 and CD45.2-PerCPcy5.5 were used for competitive BM transplantation. AnnexinV-PEcy7, Ki67-PEcy7, and DAPI were used for measuring apoptosis or proliferation. All antibodies were from eBioscience or BD Biosciences.
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