Establishment of SSCs from Oct4-GFP/LacZ and Oct4-GFP transgenic mice (C57BL/6 background) was described previously (Ko et al. 2009 (link); Kanatsu-Shinohara et al. 2011 (link); Han et al. 2012 (link)). SSC medium for expansion was composed of StemPro-34 SFM (Gibco, Carlsbad, CA, USA) with the following supplements: StemPro (Gibco), 1× N2 (Gibco), 6 mg/ml d-(+)-glucose (Gibco), 30 mg/ml pyruvic acid (Gibco), 1 μl/ml DL-lactic acid (Sigma-Aldrich, Saint Louis, MO, USA), 5 mg/ml bovine serum albumin (BSA; Gibco), 1% fetal bovine serum (FBS; Gibco), 2 mM L-glutamine (Gibco), 50 μM β-mercaptoethanol (Gibco), 1×penicillin/streptomycin (Welgene, Gyeongsan, Korea), 1× minimal essential medium (MEM) with non-essential amino acids (Gibco), 1× MEM vitamins (Welgene), 30 ng/ml β-estradiol (Sigma), 60 ng/ml progesterone (Sigma), 20 ng/ml human EGF (Peprotech, Rocky Hill, NJ, USA), 20 ng/ml human bFGF (Peprotech), 20 ng/ml human GDNF (Peprotech), and 103 U/ml murine leukemia inhibitory factor (Prospec, Rehovot, Israel).
Murine leukemia inhibitory factor
Manufactured by ProSpec
Sourced in Israel
Murine leukemia inhibitory factor is a cytokine that plays a role in the maintenance and differentiation of embryonic stem cells. It is a protein secreted by various cell types and is involved in the regulation of cellular processes.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin streptomycin, by Welgene (2 mentions)
L glutamine, by Thermo Fisher Scientific (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Bovine serum albumin (bsa), by Thermo Fisher Scientific (2 mentions)
D glucose, by Thermo Fisher Scientific (2 mentions)
Pyruvic acid, by Thermo Fisher Scientific (2 mentions)
Stempro 34 sfm, by Thermo Fisher Scientific (2 mentions)
Human egf, by Thermo Fisher Scientific (2 mentions)
Human bfgf, by Thermo Fisher Scientific (2 mentions)
Progesterone, by Merck Group (2 mentions)
Mem vitamins, by Welgene (2 mentions)
β mercaptoethanol, by Thermo Fisher Scientific (2 mentions)
β estradiol, by Merck Group (2 mentions)
Dl lactic acid, by Merck Group (2 mentions)
Human gdnf, by Thermo Fisher Scientific (2 mentions)
Non essential amino acids (neaa), by Thermo Fisher Scientific (1 mentions)
N2 supplement, by Thermo Fisher Scientific (1 mentions)
Mem non essential amino acid, by Thermo Fisher Scientific (1 mentions)
Stempro supplement, by Thermo Fisher Scientific (1 mentions)
2 protocols using murine leukemia inhibitory factor
1
Establishment of Transgenic SSCs from Mice
2
Establishment of SSCs from Oct4-GFP/LacZ Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Establishment of SSCs from Oct4-GFP/LacZ transgenic mice (C57BL/6 background) was described previously (Ko et al., 2009 (link); 2010 (link); 2012 (link)). SSC medium for expansion was composed of StemPro-34 SFM (Gibco) with the following supplements: StemPro supplement (Gibco), 1× N2 supplement (Gibco), 6 mg/ml d-(+)-glucose (Gibco), 30 mg/ml pyruvic acid (Gibco), 1 μl/ml DL-lactic acid (Sigma-Aldrich), 5 mg/ml bovine serum albumin (BSA; Gibco), 1% fetal bovine serum (Gibco), 2 mM L-glutamine (Gibco), 50 μM β-mercaptoethanol (Gibco), 1×penicillin/streptomycin (Welgene), 1× minimal essential medium (MEM) non-essential amino acids (Gibco), 1× MEM vitamins (Welgene), 30 ng/ml β-estradiol (Sigma-Aldrich), 60 ng/ml progesterone (Sigma-Aldrich), 20 ng/ml human EGF (Peprotech), 20 ng/ml human bFGF (Peprotech), 20 ng/ml human GDNF (Peprotech), and 103 U/ml murine leukemia inhibitory factor (Prospec).
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