LC-MS grade ACN, TFA, formic acid (FA), EDTA, Tris, glycerol, urea, thiourea and DTT were from Scharlab (Scharlab S. L., Barcelona, Spain). Ultrapure grade water was from Millipore (EMD Millipore Co., Billerica, MA, USA).
Sucrose, protease inhibitor cocktail (P8340) and ammonium bicarbonate were from Sigma-Aldrich (Sigma-Aldrich Co., St. Louis, MO, USA). Bradford Protein Assay Kit was from Bio-Rad (Bio-Rad, Hercules, CA, USA). Immobilized pH gradient gel (IPG) strips and IPG buffer pH 3-10 were from GE healthcare (GE healthcare, Uppsala, Sweden). Modified trypsin was from Promega (Promega, Madison, WI, USA).
Sucrose, protease inhibitor cocktail (P8340) and ammonium bicarbonate were from Sigma-Aldrich (Sigma-Aldrich Co., St. Louis, MO, USA). Bradford Protein Assay Kit was from Bio-Rad (Bio-Rad, Hercules, CA, USA). Immobilized pH gradient gel (IPG) strips and IPG buffer pH 3-10 were from GE healthcare (GE healthcare, Uppsala, Sweden). Modified trypsin was from Promega (Promega, Madison, WI, USA).