The lentiviral vectors LV-PTGS2 and CON238 were generated by Genechem Co., Ltd., Shanghai, China. LV-PTGS2 encodes the target COX-2 gene, and CON238 was used as the control vector. HOK cells were seeded in 6-well plates and infected with either LV-PTGS2 or CON238 at an MOI of 5:1 by enhanced infection solution containing polybrene (5 μg/mL). The cells were cultured in DMEM containing 10% FBS, and 48 h after infection, puromycin was added to the medium at a concentration of 2.0 μg/mL. Cells with a transfection efficiency of greater than 70% were used for experiments after confirmation. The HOK cell line variant with COX-2 overexpression was named OE, and the control variant was named NC.
Lv ptgs2
Manufactured by Genechem
Sourced in China
LV-PTGS2 is a laboratory instrument designed for the lentiviral-mediated delivery of short hairpin RNA (shRNA) for the downregulation of the PTGS2 gene. The core function of this product is to facilitate the stable expression of PTGS2-targeting shRNA in cultured cells.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using lv ptgs2
1
Generating COX-2 Overexpressing Cell Line
2
Lentiviral Modulation of SFRP1 and PTGS2
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Human LV-CON, LV-SFRP1, LV-SFRP1-RNAi, LV-PTGS2 and LV-PTGS2-RNAi were purchased from Genechem (Shanghai, China). Then, the hPTMSCs and A549 cells were infected with LV-CON, LV-SFRP1, LV-SFRP1-RNAi, LV-PTGS2 and LV-PTGS2-RNAi, repectively. SFRP1-targeting small hairpin RNA (shRNA) (ACCTTTCAGTCCGTGTTTA) and PTGS2-targeting shRNA (TGAATTTAACACCCTCTAT) were cloned into the GV Lentivirus plasmid (Genechem, China). The scramble sequence (TTCTCCGAACGTGTCACGT) of LV-CON was a control, nonspeci c shRNA that does not complement any human gene and is not toxic to cultured human cells.
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