Recombinant mouse IL-22 was purchased from Novoprotein Scientific Inc. (Shanghai, China). Anti-FoxM1 antibody was obtained from Proteintech Group, Inc. (Rosemont, IL, United States). Mouse anti–IL-22 antibody and Recombinant mouse IL-22BP protein were obtained from R&D Systems (Minneapolis, MN, United States). Anti-STAT3, anti–phospho-STAT3 (Tyr 705), anti-CyclinD1, and anti-PCNA antibodies were purchased from Cell Signaling Technology (Danvers, MA, United States). A BrdU in situ detection kit was obtained from Becton, Dickinson, and Company (Franklin Lakes, NJ, United States) and a mouse IL-22 enzyme-linked immunosorbent assay (ELISA) kit was obtained from Beijing 4A Biotech Co., Ltd. (Beijing, China).
Mouse anti il 22 antibody
Manufactured by R&D Systems
Sourced in United States
The Mouse anti–IL-22 antibody is a reagent used for the detection and quantification of interleukin-22 (IL-22) in biological samples. IL-22 is a cytokine that plays a role in immune and inflammatory responses.
Automatically generated - may contain errors
Lab products found in correlation
Recombinant mouse il 22, by Novoprotein (2 mentions)
Anti stat3, by Cell Signaling Technology (2 mentions)
Intesticult organoid growth medium, by STEMCELL (2 mentions)
Gentle cell dissociation reagent (gcdr), by STEMCELL (2 mentions)
Brdu in situ detection kit, by BD (1 mentions)
Anti foxm1 antibody, by Proteintech (1 mentions)
Anti phospho stat3 tyr705, by Cell Signaling Technology (1 mentions)
Anti cyclin d1, by Cell Signaling Technology (1 mentions)
4 protocols using mouse anti il 22 antibody
1
Investigating IL-22 Signaling Pathway
2
Investigating IL-22 and STAT3 Signaling
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Recombinant mouse IL-22 was obtained from Novoprotein Scientific Inc. (Shanghai, China). Mouse anti-IL-22 antibody was purchased from R&D Systems (USA). Anti-STAT3, anti-phospho-STAT3 (Tyr 705), and anti-cyclinD1 antibodies were purchased from Cell Signaling Technology (USA).
3
Intestinal Organoid Co-culture Assay
4
Intestinal Organoid Co-culture Assay
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IntestiCult™ Organoid Growth Medium and Gentle Cell Dissociation Reagent were purchased from StemCell. Intestinal crypts were isolated from C57BL/6J mice according to the manufacturer’s instructions and were added to previously thawed, ice-cold Matrigel at a 1:1 ratio and at a final concentration of 5,000-7,000 crypts/mL. 15µL of this mix was plated per well of a 96 well round-bottom plate. After Matrigel solidification 100µL of growth medium (100U/mL penicillin/streptomycin) was added and replaced every 3 days. Organoids were grown at 37ºC with 5% CO2 and passaged according to the manufacturer’s instructions. Freshly sorted intestinal ILC3 were added to 5-8 days old epithelial organoids after plating for 24 hours with or without anti-mouse IL-22 antibody (R&D Systems).
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