Apc anti mouse f4 80 clone bm8

Total leukocytes from naïve C57BL/6 muscle were isolated as described above and multiple populations identified using a CytoFLEX benchtop flow cytometer equipped with 405, 488, 561, and 637 nm solid phase lasers (Beckman Coulter Life Sciences, Brea, CA, USA) using the following fluorescent mAb (BioLegend):
FITC anti-mouse Ter119, PE/Cyanine7 anti-mouse Ly-6A/E (Sca-1), PE anti-mouse CD284 (TLR4), APC/Cy7 anti-mouse CD45, Brilliant Violet 421 anti-mouse CD31, and Zombie Aqua Fixable Viability Kit, as well as eFluor 660 anti-mouse/human CD34 (Thermo Fisher Scientific) (antibodies listed in Supplemental Table S2). At 4 days post-surgery, total leukocytes from muscle were isolated using a skeletal muscle dissociation kit (Miltenyi Biotech). Total leukocytes were subsequently discriminated into multiple populations using a CytoFLEX benchtop flow cytometer using the following mAb (BioLegend; outlined in Supplemental Table S1): FITC antimouse Ter119, FITC anti-mouse B220, FITC anti-mouse CD3ε, APC anti-mouse F4/80 (clone BM8), PE/Cy7 anti-mouse Ly6G, Brilliant Violet 510 anti-mouse/human CD11b, Brilliant Violet 785 anti-mouse CD45, and BD Horizon Fixable Viability Stain 700 (Supplemental Table S1). Files were subsequently analyzed with Flow Jo software version 10.5 (Becton Dickinson & Company, San Diego, CA, USA).