Mouse anti his

Western blots were performed according to described methods^{63 ,64 (link)}. Protein samples resolved by SDS-PAGE were transferred onto polyvinylidene fluoride membranes (Millipore). After blocking with QuickBlock™ Blocking Buffer (Shanghai Beyotime Biotechnology, China) for 8 h at 4 °C, membranes were incubated overnight at 4 °C with the appropriate primary antibody: rabbit anti-CccR (Laboratory preparation), 1:1000; rabbit anti-GST (Santa Cruz, cat# 53909), 1:1000; rabbit anti-RNAP (Santa Cruz, cat# sc-56766), 1:5000; mouse anti-His (Santa Cruz, cat# sc-8036), 1:5000. The membranes were then washed five times with TBST buffer (10 mM Tris-HCl, 150 mM NaCl, 0.05% Tween, pH 7.5) and incubated with 1:10,000 dilution of goat anti-rabbit horseradish peroxidase-conjugated secondary antibodies (DIYIBIO, China, cat# DY60202) or goat anti-mouse horseradish peroxidase-conjugated secondary antibodies (DIYIBIO, China, cat# DY60203) at 4 °C for 4 h. After another seven washes with TBST buffer, chemiluminescent signals were detected by using the ECL Plus Kit (GE Healthcare). Uncropped images of blots can be found in the Source Data file.

The following primary antibodies were used in western blot, immunofluorescence or chromatin immunoprecipitation assays as indicated: mouse anti-c-Myc (ThermoFisher cat#9E10), mouse anti-His (Santa Cruz cat#sc57598), rabbit anti-EZH2 (Invitrogen cat#36–6300), rabbit anti-SUZ12 (Cell signaling cat#D39F6), rabbit anti-H3k27me3 (Active motif Cat#39155), rabbit anti-H3k4me3 (EMD Millipore cat#07–473), rabbit anti-βactin (Abcam cat#ab8227), rabbit anti-Histone H3 (Abcam cat#ab1791), mouse anti-Tax (cat#168-A51 from the National Institutes of Health AIDS Research and Reference Reagent Program), mouse anti-HBZ (a gift from J.M. Péloponèse), rabbit anti-E(z), rabbit anti-SUZ12 (a gift from J. Müller), mouse anti-Relish (DSHB cat#C21F3) and anti-GAPDH (B2534M-HRP Abnova), mouse anti-c-Myc-tag (9E10) (Abcam cat#ab32), rabbit anti-EZH2 (Merck Millipore cat# 07–689), Goat anti-mouse Alexa Fluor-488 (Abcam cat≠150113), Goat anti rabbit -rabbit Alexa Fluor-594 (Abcam cat ≠150080).