We have previously described the GFP-SNX1 and GFP-Rab5 plasmids used in this study [16 (link)]. Monoclonal anti-GM130 (#610822) was from BD Biosciences (San Jose, CA, USA). Tamoxifen (TAM) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Derivative compounds were synthesized at the Center for Innovative Drug Discovery (University of Texas at San Antonio, San Antonio, TX, USA; see Supplementary Materials ). All compounds were dissolved in DMSO and used at 10 µM final concentration unless specified otherwise.
STx2 holotoxin and STx2B were obtained from BEI Resources (Manassas, VA, USA). For transport assays, STx2B was labeled using Alexa Fluor™ 555 Microscale Protein Labeling Kit using manufacturer’s instructions (ThermoFischer, Waltham, MA, USA).
STx2 holotoxin and STx2B were obtained from BEI Resources (Manassas, VA, USA). For transport assays, STx2B was labeled using Alexa Fluor™ 555 Microscale Protein Labeling Kit using manufacturer’s instructions (ThermoFischer, Waltham, MA, USA).