Five hundred microliters of blood by heart puncture was put into sterile tubes after coagulation and then centrifuged at 3,000 rpm for 10 min at 4°C. The upper serum was obtained and stored at –80°C. Afterward, the contents of EPO (Cloud-Clone Corp, Lot No. L161124009), TPO (Cloud-Clone Corp, Lot No. L161130210), and GM-CSF (ExCell Bio, Lot No. 21S343) were detected using enzyme-linked immunosorbent assay (ELISA) kits (Cloud-Clone Corp, Lot No. L161124009).
Gm csf
Manufactured by ExCell Bio
Sourced in China
GM-CSF is a laboratory-produced cytokine protein that acts as a growth factor, stimulating the production and activation of granulocytes and macrophages. It plays a key role in the regulation of immune and inflammatory responses.
Automatically generated - may contain errors
Lab products found in correlation
Elisa kit, by Cloud-Clone (1 mentions)
Il 1β, by ExCell Bio (1 mentions)
Interleukin 6 (il 6), by ExCell Bio (1 mentions)
Granzyme b, by Thermo Fisher Scientific (1 mentions)
Lipofectamine 2000 transduction reagent, by Thermo Fisher Scientific (1 mentions)
U bottom 96 well plate, by Thermo Fisher Scientific (1 mentions)
Nlvpmvatv, by ProImmune (1 mentions)
3 protocols using gm csf
1
Measurement of Serum EPO, TPO, and GM-CSF
2
Lung Cytokine Assay Protocol
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Lungs (right lobe) were homogenized in 2 ml PBS + 0.05% Tween 80. Homogenized tissue supernatants were filtered (0.22 μ m). Cell culture supernatants were collected. Cytokine production was measured by enzyme-linked immunosorbent assay of mouse IFN-γ, TNF-α, GM-CSF, nitrate, IL-6, IL-1β or IL-2 (ExCell Bio, China) according to the manufacturer’s protocol.
3
T Cell Cytokine Reactivity Assay
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CD8+ cells were tested for reactivity in cytokine-release assays using ELISA kits [IFN-γ, TNF-α, granulocyte-macrophage colony-stimulating factor (GM-CSF); ExCell Bio, Shanghai, China; granzyme B (GrB); eBioscience, San Diego, California, USA]. T2 were pulsed with HLA-A∗0201-restricted MTB Ag85B199–207 (KLVANNTRL), HIV-1 Env120–128 (KLTPLCVTL) or CMV pp65495–503 peptide (NLVPMVATV) (Proimmune, Oxford, UK) at indicated concentrations for 3 h at 37°C. Stimulator cells and responder cells were cocultured in a 96-well U-bottom plate (Nunc) for 24 h, except IFN-γ, which was detected after 18 h of incubation. In some groups, DCs were transfected with the pV1J.ns-tPA-Ag85B (gifted by Dr Kris Huygen in Pasteur Institute of Brussels, Brussels, Belgium) or the pCAGGS-Env plasmid (gifted by Dr James M. Binley in Torrey Pines Institute for Molecular Studies, San Diego, California, USA) respectively using Lipofectamine 2000 Transduction Reagent (Invitrogen, Carlsbad, California, USA).
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