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3 protocols using chlorocresol

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Multi-Ingredient Formulation Protocol

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Glycoluril, hydrochloric acid (37%), 1,6-dibromohexane, deuterium oxide (99.9%), deuterium chloride (35%), isoquinoline and chlorocresol (4-chloro-3-methyphenol) were purchased from Sigma-Aldrich. Paraformaldehyde was purchased from AlfaAesar. Phenoxyethanol, zinc oxide BP, lanolin anhydrous and citric acid monohydrate were purchased from the Sydney Essential Oil Company. Cetostearyl alcohol was purchased from Bronson and Jacobs. White soft paraffin was supplied by The University of Sydney Dispensing Laboratory. Sodium phosphate BP was purchased from the Government Store Department. 1,4-dioxane, calcium hydroxide solution and sulfuric acid were purchased from Merck. Arachis oil was purchased from Proteco and oleic acid from Professional Compounding Chemists of Australia. All water was obtained from an SG ultra clear water system. Phosphate buffered saline (PBS) solutions were made using pure water and PBS tablets from Sigma Life Sciences. All other chemicals including: methanol, ethanol and diethyl ether, were of analytical reagent grade.
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2

Cellular Stress Signaling Pathway Antibodies

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Ubiquitin (sc-8017), LAMP1 (sc-20011), phospho-PERK (sc-32577), PERK (sc-13073), phospho-c-Jun (sc-822), c-Jun (sc-376488), SERCA2 (sc-376235, sc-8095), ATF6 (sc-22799), eIF2α (sc-11386), CaMKII (sc-5306), α1C/Cav1.2 (sc-16229-R), α1D/Cav1.3 (sc-25687), LC3 (sc-271625) and p62 (sc-28359) antibodies were obtained from Santa Cruz Biotechnology, Inc. IRE1α (3294), phospho-CaMKII (12716), phospho-eIF2α (3398), PERK (5683), LC3 (2775), p62 (5114) and Calnexin (2679) antibodies were purchased from Cell Signaling Technology, Inc. Pan-keratin antibody was obtained from Dr. Omary (University of Michigan)65 (link). Actin (JLA20) and tubulin (T5168) antibodies were purchased from Developmental Studies Hybridoma Bank (DSHB) and Sigma, respectively. Fatty acid free and low endotoxin bovine serum albumin (BSA), palmitic acid (PA), stearic acid (SA), oleic acid (OA), docosahexaenoic acid (DHA), tunicamycin (Tm), thapsigargin (Tg), butylated hydroxyanisole (BHA), N-acetylcysteine (NAC), chlorocresol, L-cycloserine, glucagon, phenylephrine, vasopressin, verapamil and nicardipine were purchased from Sigma. SP600125, bafilomycin and rapamycin were from LC Labs, and fumonisin B1 and suramin were from Cayman Chemical.
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3

Cellular Stress Signaling Pathway Antibodies

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Ubiquitin (sc-8017), LAMP1 (sc-20011), phospho-PERK (sc-32577), PERK (sc-13073), phospho-c-Jun (sc-822), c-Jun (sc-376488), SERCA2 (sc-376235, sc-8095), ATF6 (sc-22799), eIF2α (sc-11386), CaMKII (sc-5306), α1C/Cav1.2 (sc-16229-R), α1D/Cav1.3 (sc-25687), LC3 (sc-271625) and p62 (sc-28359) antibodies were obtained from Santa Cruz Biotechnology, Inc. IRE1α (3294), phospho-CaMKII (12716), phospho-eIF2α (3398), PERK (5683), LC3 (2775), p62 (5114) and Calnexin (2679) antibodies were purchased from Cell Signaling Technology, Inc. Pan-keratin antibody was obtained from Dr. Omary (University of Michigan)65 (link). Actin (JLA20) and tubulin (T5168) antibodies were purchased from Developmental Studies Hybridoma Bank (DSHB) and Sigma, respectively. Fatty acid free and low endotoxin bovine serum albumin (BSA), palmitic acid (PA), stearic acid (SA), oleic acid (OA), docosahexaenoic acid (DHA), tunicamycin (Tm), thapsigargin (Tg), butylated hydroxyanisole (BHA), N-acetylcysteine (NAC), chlorocresol, L-cycloserine, glucagon, phenylephrine, vasopressin, verapamil and nicardipine were purchased from Sigma. SP600125, bafilomycin and rapamycin were from LC Labs, and fumonisin B1 and suramin were from Cayman Chemical.
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