Single-cell suspensions of iMyoblasts and bMyoblasts cultures dissociated with TrypLE Express Enzyme (Thermo Fisher Scientific) were washed with PBS, filtered with a 40-μm strainer, and incubated with antibodies suspended in PBS for 30–60 min on ice in the dark. Cells were then washed in PBS and resuspended in PBS and 0.2% fetal calf serum, and flow cytometry was performed at UMass Medical School Flow Cytometry Core. A BD FACS Aria IIu was used for quantification and a BD FACS C-Aria II Cell Sorter was used for cell sorting. FlowJo software was used for data analysis.
Facs c aria 2 cell sorter
Manufactured by BD
The BD FACS C-Aria II Cell Sorter is a multi-laser, high-speed cell sorting instrument designed for analyzing and sorting a wide range of cell types. It features advanced optics and detector technology for precise cell identification and sorting.
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Lab products found in correlation
3 protocols using facs c aria 2 cell sorter
1
Single-cell Immunophenotyping of Myoblasts
2
Flow Cytometry Analysis of Hedgehog Signaling
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For flow sorting, pelleted cells were resuspended in the corresponding media and sorted into tubes or 96-well plates with a BD FACS C-Aria II Cell Sorter (BSL-2+/BSC). For flow analysis, cells were seeded at nearly confluent densities in 12-well plates, serum starved for 24 h, and treated with or without SAG or SHH conditioned media for 48 h. Cells were trypsinized, pelleted, and then resuspended in 500 µl 1× PBS. Flow analysis was performed with a digital multiparameter flow cytometer analyzer (LSR II; BD Biosciences). Data were processed in FlowJo version 10. Ridgeline plots were drawn in RStudio with ggplot2 and ggridges (https://github.com/clauswilke/ggridges ) in R 3.5.3.
3
Cell Sorting Protocol for Downstream Analysis
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For flow sorting, pelleted cells were resuspended in the corresponding media and sorted into tubes or 96-well plates containing media with 10% fetal bovine serum by a BD FACS C-Aria II Cell Sorter (BSL-2+/BSC).
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