Hrp tagged igg secondary antibody

DMEM medium, fetal bovine serum (FBS), EDTA, and penicillin-streptomycin were purchased from Hyclone; dimethyl sulfoxide and MTT were purchased from Gibco; trypsin-EDTA was purchased from Sigma; PVDF membrane was purchased from Pall Life Sciences; Western blot-related chemical reagents were from Shanghai Beyotime biotechnology Co. LTD; ECL reagent was purchased from Amersham Biosciences; DDR-1 monoclonal antibody and HRP tagged IgG secondary antibody were purchased from Cell Signaling; nilotinib and transwell chambers were purchased from Sigma; MMP-2 and MMP-9 ELISA kits were purchased from R&D; other reagents were purchased from Shanghai Sangon Biotechnology Co., LTD; and Labsystem Version1.3.1 microplate reader was purchased from Bio-rad.

A549 and A549/DDP cell lines were purchased from the ATCC Cell Bank (USA). DMEM medium, fetal bovine serum (FBS), EDTA, and penicillin-streptomycin were bought from Hyclone (USA). Dimethyl sulfoxide and MTT powder were from Gibco. Tyresin-EDTA was from Sigma (USA). PVDF membrane was from Pall Life Sciences. Western blot-related chemical reagents were obtained from Beyotime. ECL reagent was from Amersham Biosciences. ERCC1, BRCA1, PI3K, AKT, phosphorylated PI3K, and phosphorylated AKT monoclonal antibodies, and HRP-tagged IgG secondary antibody were from Cell Signaling. Caspase 3 activity kits were purchased from Pall Life Sciences. RNA extraction kits and reverse transcription kits were from Axygen (USA). Other common reagents were purchased from Sangon. The Labsystem version 1.3.1 microplate reader was purchased from Bio-Rad (USA).