Antibodies and their manufacturers were: mouse anti-FLAG clone M2 (WB 1 : 4000, IF 1 : 400; F1804) and rabbit anti-LC3B (WB 1 : 1000, IF 1 : 200; L7543) were from Sigma–Aldrich; goat anti-FLAG (1 : 400; ab1257), rabbit anti-SQSTM1/p62 (IP 1–2 μg/mg of lysate; ab101266) and rabbit anti-GFP (WB 1 : 2000, ab290) were from Abcam; mouse anti-SQSTM1/p62 (WB 1 : 1000 IF 1 : 200; 610832) was from BD Biosciences; rabbit anti-SQSTM1/p62 (WB 1 : 1000, 8025S) was from Cell Signaling Technology; mouse anti-HA.11 clone 16B12 (WB 1 : 1000, 901501) was from Biolegend; mouse anti-HA clone 12CA5 (WB 1 : 2000; 11583816001) from Roche; mouse IgG isotype control (14-4732-82) was from Invitrogen; Alexa Fluor 488-, 555-, 594- and 647-conjugated secondary antibodies were from Thermo Fisher (A21206, A31570, A11032 and A21447, respectively).
Mouse anti sqstm1 p62
Manufactured by BD
Mouse anti-SQSTM1/p62 is a monoclonal antibody that detects the SQSTM1/p62 protein. SQSTM1/p62 is a multifunctional protein involved in various cellular processes.
Automatically generated - may contain errors
Lab products found in correlation
A11032, by Thermo Fisher Scientific (2 mentions)
Rabbit anti sqstm1 p62, by Cell Signaling Technology (2 mentions)
A21206, by Thermo Fisher Scientific (2 mentions)
Mouse igg isotype control 14 4732 82, by Thermo Fisher Scientific (2 mentions)
A31570, by Thermo Fisher Scientific (2 mentions)
Mouse anti ha clone 12ca5, by Roche (2 mentions)
A21447, by Thermo Fisher Scientific (2 mentions)
Mouse anti flag clone m2, by Merck Group (2 mentions)
Rabbit anti lc3b, by Merck Group (2 mentions)
Mouse anti ha 11 clone 16b12, by BioLegend (1 mentions)
Rabbit anti gfp, by Abcam (1 mentions)
Goat anti flag, by Merck Group (1 mentions)
Rabbit anti p62 sqstm1, by Abcam (1 mentions)
Ab1257, by Abcam (1 mentions)
Ab101266, by Abcam (1 mentions)
Ab290, by Abcam (1 mentions)
Ab172730, by Abcam (1 mentions)
2 protocols using mouse anti sqstm1 p62
1
Antibody Characterization for LC3B and p62
2
Antibody Characterization for Western Blot and Immunofluorescence
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Antibodies and their manufacturers were: rat anti-BPLF1 [56 (link)] (WB 1:1600, 2E5 supernatant) was from Helmholtz Zentrum (2E5); mouse anti-FLAG clone M2 (WB 1:4000, IF 1:400; F1804) and rabbit anti-LC3B (WB 1:1000, IF 1:200; L7543) were from Sigma-Aldrich; goat anti-FLAG (1:400; ab1257) and rabbit anti-SQSTM1/p62 (IP 1–2 μg/mg of lysate; ab101266), rabbit anti-GFP (WB 1:2000, ab290) and rabbit IgG isotype control (ab172730) were from Abcam; mouse anti-SQSTM1/p62 (WB 1:1000 IF 1:200; 610,832) was from BD Biosciences; rabbit anti-SQSTM1/p62 (WB 1:1000, 8025S) was from Cell Signaling Technology; mouse anti-HA clone 12 CA5 (WB 1:2000; 11,583,816,001) from Roche; mouse IgG isotype control (14–4732-82) was from Invitrogen; Alexa Fluor 488-, 555-, 594- and 647-conjugated secondary antibodies were from Thermo Fisher (A21206, A31570, A11032 and A21447, respectively).
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