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Aav9 cag flex rg

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AAV9-CAG-FLEX-RG is an adeno-associated virus (AAV) vector that contains a Cre-dependent gene of interest under the control of the CAG promoter. The core function of this vector is to enable Cre-mediated expression of the gene of interest.

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Lab products found in correlation

Aav9 ef1a flex tva mcherry, by Addgene (5 mentions) Enva sad δg egfp, by Addgene (5 mentions) Aav2 retro cag icre, by Addgene (3 mentions) Aav5 syn flex chrimsonr tdtomato, by Addgene (3 mentions) Aav5 hsyn flex chrimsonr tdtomato, by Addgene (2 mentions) Aav9 cag flex tdtomato, by Addgene (2 mentions) Aav9 syn flex jgcamp7s wpre, by Addgene (2 mentions) Aav2 ef1a flex off hchr2 h134r eyfp, by Vector Biolabs (2 mentions) Aav2 ef1a flex off hm3dq mcherry, by Vector Biolabs (2 mentions) Aav2 hsyn dio hm3d gq mcherry, by Addgene (1 mentions) Aav2 hsyn dio hm4di mcherry, by Addgene (1 mentions)

6 protocols using aav9 cag flex rg

1

Viral Vector Production and Characterization

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AAV9-hSyn-Flex-GCaMP6s-WPRE-SV40 was produced by the Vector Core of the University of Pennsylvania. AAV9-EF1α-DIO-eNpHR3.0-mCherry, AAV2-CaMKII2α-eNpHR3.0-mCherry, AAV2-CaMKII2α-mCherry, AAV2-EF1α-DIO-hChR2(H134R)-YFP-WPRE and AAV2-EF1α-DIO-mCherry were produced by the Vector Core of the University of North Carolina. AAV5-hSyn-FLEX-ChrimsonR-tdTomato (Addgene plasmid 62723), AAV9-CAG-FLEX-tdTomato (Addgene plasmid # 28306) and AAV9-Syn-Flex-jGCaMP7s-WPRE (Addgene plasmid # 104491) were purchased from Addgene. AAV2(retro)-CAG-iCre (Addgene plasmid # 81070) was produced by Vector Biolabs. AAV9-EF1a-FLEX-TVA-mCherry (Addgene plasmid # 38044) and AAV9-CAG-FLEX-RG (Addgene plasmid # 38043) were produced by Vigene Biosciences, Inc. EnvA-SAD-ΔG-eGFP (Addgene plasmid # 32635) was produced by the Viral Vector Core of the Salk Institute for Biological Studies. All viral vectors were stored in aliquots at −80°C until use.
Ma J., du Hoffmann J., Kindel M., Sofia Beas B., Chudasama Y, & Penzo M.A. (2021). Divergent projections of the paraventricular nucleus of the thalamus mediate the selection of passive and active defensive behaviors. Nature neuroscience, 24(10), 1429-1440.
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2

Viral Vector Production and Characterization

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AAV9-hSyn-Flex-GCaMP6s-WPRE-SV40 was produced by the Vector Core of the University of Pennsylvania. AAV9-EF1α-DIO-eNpHR3.0-mCherry, AAV2-CaMKII2α-eNpHR3.0-mCherry, AAV2-CaMKII2α-mCherry, AAV2-EF1α-DIO-hChR2(H134R)-YFP-WPRE and AAV2-EF1α-DIO-mCherry were produced by the Vector Core of the University of North Carolina. AAV5-hSyn-FLEX-ChrimsonR-tdTomato (Addgene plasmid 62723), AAV9-CAG-FLEX-tdTomato (Addgene plasmid # 28306) and AAV9-Syn-Flex-jGCaMP7s-WPRE (Addgene plasmid # 104491) were purchased from Addgene. AAV2(retro)-CAG-iCre (Addgene plasmid # 81070) was produced by Vector Biolabs. AAV9-EF1a-FLEX-TVA-mCherry (Addgene plasmid # 38044) and AAV9-CAG-FLEX-RG (Addgene plasmid # 38043) were produced by Vigene Biosciences, Inc. EnvA-SAD-ΔG-eGFP (Addgene plasmid # 32635) was produced by the Viral Vector Core of the Salk Institute for Biological Studies. All viral vectors were stored in aliquots at −80°C until use.
Ma J., du Hoffmann J., Kindel M., Sofia Beas B., Chudasama Y, & Penzo M.A. (2021). Divergent projections of the paraventricular nucleus of the thalamus mediate the selection of passive and active defensive behaviors. Nature neuroscience, 24(10), 1429-1440.
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3

Viral Tracing of Mouse Neural Circuits

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Animal experiments were carried out following guidelines of the Stockholm municipal committee, the Scripps Research Institutional Animal Care and Use Committee and the Stanford University Institutional review panel approval. All viral injections into the striatum (Drd1a-cre (EY262)50 (link) n = 4, Drd2-cre (ER44)50 (link) n = 3, Adora2a-cre [GENSAT: KG139] n = 1, Chat-IRES-cre [Jax: 006410] n = 4) were done at the following coordinates from bregma AP: +0.6 mm, ML: +2.2 mm, DV: −3.5 to −3.2 mm (500 nl). Injections into the motor cortex (Camk2a-cre [Jax: 005359] n = 4, Gad2-cre [Jax: 010802] n = 4) were done at AP: 1.5 mm, ML: 1.5 mm, DV: −0.4 mm (500 nl). Injections were performed in 2–6 months old mice under isoflurane anesthesia and injected with buprenorphine post surgery. In all mice the second injection was performed after 2–4 weeks with (EnvA)SADΔG-EGFP or (EnvA)SADΔG-ChR2(H134R)-mCherry (Adora2a-cre n = 1, D1-cre n = 2, Chat-cre n = 3) at the same location, for the striatal injections we either used AAV5-TVA-t2a-RG as or a dualvector helper virus system (AAV5-EF1a-DIO-TVA-mCherry and AAV5-EF1a-DIO-RG, 1:1 ratio). In experiments with Camk2a-cre and Gad2-cre animals we used AAV9-CAGFLEXRG [Addgene plasmid 48333] and AAV9-CAG-FLEX-TVA-mCherry [Addgene plasmid 48332] in 1:1 ratio.
Fürth D., Vaissière T., Tzortzi O., Xuan Y., Märtin A., Lazaridis I., Spigolon G., Fisone G., Tomer R., Deisseroth K., Carlén M., Miller C.A., Rumbaugh G, & Meletis K. (2017). An interactive framework for whole-brain maps at cellular resolution. Nature neuroscience, 21(1), 139-149.
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4

Viral Vector Delivery Techniques

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AAV9-Syn-Flex-GCaMP6s-WPRE-SV40 was produced by the Vector Core of the University of Pennsylvania. AAV5-Syn-FLEX-ChrimsonR-tdTomato (Addgene plasmid # 62723) was produced by Addgene. AAV2-EF1a-FLEX/OFF-hChR2(H134R)-eYFP and AAV2-EF1a-FLEX/OFF-hM3Dq-mCherry were produced by Vector Biolabs. AAV9-Syn-DO-GCaMP6s and AAV9-Syn-DO-oScarlett were produced by Charu Ramakrishnan (Deisseroth’s lab, Stanford University). CAV2-Cre was produced by the Adenovirus Vector Core of the Institut de Genetique Moleculaire de Montpellier. AAV9-EF1a-DIO-eNpHR3.0-mCherry, AAV2-CaMKIIa-mCherry, AAV2-EF1a-DIO-hChR2(H134R)-EYFP, and AAV2-EF1a-DIO-mCherry were produced by the Vector Core of the University of North Carolina. AAV9-EF1a-FLEX-TVA-mCherry (Addgene plasmid # 38044) and AAV9-CAG-FLEX-RG (Addgene plasmid # 38043) were produced by Vigene Biosciences, Inc. EnvA-SAD-ΔG-eGFP (Addgene plasmid # 32635) was produced by the Viral Vector Core of the Salk Institute for Biological Studies. All viral vectors were stored in aliquots at −80°C until use. The CreOFF viral vectors (DO and FLEX/OFF) used in this study are fully available from the authors upon request.
Gao C., Leng Y., Ma J., Rooke V., Rodriguez-Gonzalez S., Ramakrishnan C., Deisseroth K, & Penzo M.A. (2020). Two genetically, anatomically and functionally-distinct cell types segregate across the antero-posterior axis of the paraventricular thalamus. Nature neuroscience, 23(2), 217-228.
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5

Viral Vector Delivery Techniques

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AAV9-Syn-Flex-GCaMP6s-WPRE-SV40 was produced by the Vector Core of the University of Pennsylvania. AAV5-Syn-FLEX-ChrimsonR-tdTomato (Addgene plasmid # 62723) was produced by Addgene. AAV2-EF1a-FLEX/OFF-hChR2(H134R)-eYFP and AAV2-EF1a-FLEX/OFF-hM3Dq-mCherry were produced by Vector Biolabs. AAV9-Syn-DO-GCaMP6s and AAV9-Syn-DO-oScarlett were produced by Charu Ramakrishnan (Deisseroth’s lab, Stanford University). CAV2-Cre was produced by the Adenovirus Vector Core of the Institut de Genetique Moleculaire de Montpellier. AAV9-EF1a-DIO-eNpHR3.0-mCherry, AAV2-CaMKIIa-mCherry, AAV2-EF1a-DIO-hChR2(H134R)-EYFP, and AAV2-EF1a-DIO-mCherry were produced by the Vector Core of the University of North Carolina. AAV9-EF1a-FLEX-TVA-mCherry (Addgene plasmid # 38044) and AAV9-CAG-FLEX-RG (Addgene plasmid # 38043) were produced by Vigene Biosciences, Inc. EnvA-SAD-ΔG-eGFP (Addgene plasmid # 32635) was produced by the Viral Vector Core of the Salk Institute for Biological Studies. All viral vectors were stored in aliquots at −80°C until use. The CreOFF viral vectors (DO and FLEX/OFF) used in this study are fully available from the authors upon request.
Gao C., Leng Y., Ma J., Rooke V., Rodriguez-Gonzalez S., Ramakrishnan C., Deisseroth K, & Penzo M.A. (2020). Two genetically, anatomically and functionally-distinct cell types segregate across the antero-posterior axis of the paraventricular thalamus. Nature neuroscience, 23(2), 217-228.
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6

Viral Vector Toolkit for Neuroscience

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AAV2/5-Ef1a-DIO hChR2(E123T/T159C)-EYFP and AAV9-hSyn-FLEX-GCaMP6s-WPRE-SV40 were produced by the Vector Core of the University of Pennsylvania. AAV9-EF1a-DIO-eNpHR3.0-mCherry, AA9-CAG-FLEX-tdTomato, AV9-hSyn-DIO-mCherry-2A-SyneGFP and AAV2-Syn-DIO-GFP were produced by the Vector Core of the University of North Carolina. AAV5-Syn-FLEX-ChrimsonR-tdTomato (Addgene plasmid # 62723) and AAV2-hSyn-DIO-hM4Di-mCherry, AAV2-hSyn-DIO-hM3D(Gq)-mCherry were produced by Addgene. AAV2(retro)-CAG-iCre (Addgene plasmid # 81070) was produced by Vector Biolabs. AAV9-EF1a-FLEX-TVA-mCherry (Addgene plasmid # 38044) and AAV9-CAG-FLEX-RG (Addgene plasmid # 38043) were produced by Vigene Biosciences, Inc. EnvA-SAD-ΔG-eGFP (Addgene plasmid # 32635) was produced by the Viral Vector Core of the Salk Institute for Biological Studies. AAV2-retro-CAG-tdTomato-WPRE is a gift from Dr. Yuanyuan Liu at NIDCR. All viral vectors were stored in aliquots at −80°C until use.
Sofia Beas B., Gu X., Leng Y., Koita O., Rodriguez-Gonzalez S., Kindel M., Matikainen-Ankney B.A., Larsen R.S., Kravitz A.V., Hoon M.A, & Penzo M.A. (2020). A ventrolateral medulla-midline thalamic circuit for hypoglycemic feeding. Nature Communications, 11, 6218.
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