The main astringent substances present during green tea infusion were detected via HPLC with a UV detector (HPLC/UV; Shimadzu, Kyoto, Japan) and we used a C18 column known as Diamonsil™ (4.6 mm × 250 mm, 5 μm; Dikma Technologies Inc., Lake Forest, CA, USA) for the separation reaction, with the column’s temperature set at 35 °C. We prepared mobile phases A of water/2% (v/v) acetic acid and mobile phases B of 100% acetonitrile, respectively. The post-run time was 5 min. The injection volume was 10 μL. There was a flow rate of 1 mL/min and a detection wavelength 280 nm. The elution gradient began with 6.5% B, which was increased to 15% B at 16 min. This level was maintained until 25 min, when it finally back to 6.5% B at 30 min.
Diamonsil
Manufactured by Dikma Technologies
Sourced in United States
Diamonsil is a laboratory equipment product designed for use in scientific analysis and research. It functions as a chromatographic column packing material, providing a high-performance liquid chromatography (HPLC) stationary phase. Diamonsil is composed of silica particles that have been chemically modified with a diamond-like carbon coating, enhancing its durability and chemical stability.
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Lab products found in correlation
4 protocols using diamonsil
1
HPLC Analysis of Green Tea Astringents
2
Salinomycin Nanoparticle Encapsulation Efficiency
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The drug encapsulation and loading efficacy of salinomycin in nanoparticles were measured by high-performance liquid chromatography (HPLC, L-2000; Hitachi), after the lyophilized nanoparticles were dissolved in dichloromethane.14 (link) A reverse-phase C-18 column (Diamonsil, 250×4.6 mm, 5 μm; Dikma Technologies, Inc, Lake Forest, CA, USA) was used. The mobile phase was acetonitrile/deionized water/tetrahydrofuran/phosphoric acid (85/10/5/0.01, v/v), and the flow rate was 1.5 mL/min. The detection wavelength and column temperature were 210 nm and 30°C, respectively. Drug-loading efficiency was calculated as ME/MN ×100%, with ME being the mass of encapsulated drugs, and MN being the mass of nanoparticles. The drug encapsulation efficiency was calculated as ME/MT ×100%, with MT being the mass of total drugs. The CFPE concentration in nanoparticles was calculated according to CFPE calibration curves constructed by standard lead CFPE solutions.
3
Quantification of 5-FU in Bel-7402/FU Cells
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Bel-7402/FU cells (5×105/ml) were exposed to 0.025 mg/ml 5-FU in the presence or absence, of 0.08 mg/ml ASIV or 0.001 mg/ml verapamil for 24 h at 37°C. Following trypsinization, the cells were extracted with 500 μl of methanol by ultrasonication and centrifuged at 12,000 × g for 30 min at 4°C. The supernatant was filtered and dried with nitrogen gas. Subsequently, the mobile phase was added to achieve a metered volume of 0.5 ml for the quantitative analysis. Analysis was performed using the Agilent 1100 high performance liquid chromatography (HPLC) system (Agilent Technologies, Santa Clara, CA, USA) comprised of a quaternary pump, an autosampler and a UV detector. A C18 column (250 × 4.6 mm, 5 μm, Diamonsil; Dikma, Lake Forest, CA, USA) was used for the separation. The flow rate was 1 ml/min with methanol:water (10:90, v/v) as the mobile phase. Peak areas were determined at 265 nm for 5-FU.
4
Quantifying Sal Nanoparticle Encapsulation
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The drug encapsulation and loading efficacy of Sal in nanoparticles were determined by high-performance liquid chromatography (HPLC, L-2000; Hitachi). HPLC was performed by a reverse-phase C-18 column (Diamonsil, 250 × 4.6 mm, 5 μm; Dikma Technologies, Inc, Lake Forest, CA, USA). The mobile phase was acetonitrile/deionized water/tetrahydrofuran/phosphoric acid (85:10:5:0.01, v/v) with the flow rate 1.0 mL/min. Drug-loading efficiency was calculated as DE/DN × 100% (DE: the mass of encapsulated Sal; DN: the mass of NPs) and the drug encapsulation efficiency was calculated as DE/DT × 100% (DT: the mass of total NPs). The FAM concentration in NPs was calculated according to FAM calibration curves constructed.
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