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Alt and ast colorimetric activity assay kits

Manufactured by Cayman Chemical
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The ALT and AST colorimetric activity assay kits are laboratory equipment designed to measure the enzymatic activity of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in various sample types. These kits utilize a colorimetric detection method to quantify the levels of these enzymes, which are commonly used as biomarkers in clinical and research applications.

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Creatinine serum colorimetric assay kit, by Cayman Chemical (1 mentions)

2 protocols using alt and ast colorimetric activity assay kits

1

Quantifying Liver Enzyme Levels

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ALT and AST levels from plasma of mice were measured with the ALT and AST colorimetric activity assay kits (Cayman Chemical, MI). ALT activity was measured by monitoring the rate of NADH oxidation in a coupled reaction using lactate dehydrogenase (LDH). The NADH to NAD+ oxidation caused a decrease in A340 nm absorbance. The rate of decrease (ΔA340/min) is directly proportional to the ALT activity. AST activity was measured by the rate of NADH oxidation in the presence of malate dehydrogenase. NADH to NAD+ conversion caused a decrease in A340 nm absorbance. LDH was added to the AST reaction to prevent interference from endogenous pyruvate in the plasma. The ΔA340/min for both ALT and AST were converted to units/L by dividing the ΔA340 values by the NADH extinction coefficient and multiplying by the sample dilution factor as per the protocol instructions (Cayman).
Ramani K., Mavila N., Abeynayake A., Tomasi M.L., Wang J., Matsuda M, & Seki E. (2022). Targeting A-kinase anchoring protein 12 phosphorylation in hepatic stellate cells regulates liver injury and fibrosis in mouse models. eLife, 11, e78430.
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2

Evaluating Nanoparticle Vaccine Efficacy

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Mice were vaccinated with 1 μg RBC-NPs, Ab-OMVs, or Ab-NPs on days 0, 7, and 14. Blood was collected from the submandibular vein, and serum was isolated on days 0 (before vaccination), 1, and 15. Serum creatinine was assessed using a creatinine (serum) colorimetric assay kit according to the manufacturer’s instructions (Cayman Chemical, 700460). Serum AST and ALT were determined using ALT and AST colorimetric activity assay kits, respectively (Cayman Chemical, 701640, 700260).
(1999). Proceedings of the National Academy of Sciences of the United States of America, 96(11), 6571.
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