Waters lct premier mass spectrometer

All chemicals and regents used for this study were analytical grade and used without further purification. 1,10-phenanthroline monohydrate (BDH chemical Ltd., Poole, England), copper (II) acetate monohydrate [Cu(CO₂CH₃)₂·H₂O], triethylamine, NaHCO₃, NaOH, and Mueller-Hinton agar were purchased from Loba Chemie PVT Ltd., Addis Ababa. Metformin hydrogen chloride (Met.HCl) and ciprofloxacin hydrogen chloride (Cip.HCl) were obtained from Cadila Pharmaceuticals PLC, Ethiopia. Methanol, ethanol, HCl, DMSO, ethyl acetate and dichloromethane (DCM) were purchased from Alpha Chemika, Addis Ababa, Ethiopia. Thin Layer Chromatography (MERCK Silica gel 60 F254) together with UV Cabinet (UV-Vis lamp at 254 and 365 nm) were used to monitor the progress of the chemical reactions. The melting points of the complexes were determined using capillary tubes (Thiele tube). UV-Vis spectrophotometer (SM-1600 Spectrophotometer), FTIR (Perkin-Elmer BX spectrometer, Shimadzu Corporation, Japan), TGA/DTC (DTG-60H SHIMADZU thermal analyzer) and High resolution mass spectra (Waters-LCT-Premier mass spectrometer) were used to characterize the synthesized metal complexes.

The infrared measurements (KBr discs) were recorded on a Perkin-Elmer BX FTIR spectrometer (4000–400). The UV-visible spectra were recorded on a SM-1600 Spectrophotometer. Molar conductance of the complexes was recorded at room temperature in 1.0 × 10-3 M in DMSO solution using electrical conductometer (AD8000). The thermogravimetric/differential thermal analyses (TGA/DTA) data were recorded from 25–800 C with a heating rate of 10°C/min under nitrogen-atmosphere (20 ml/min) by a Shimadzu DTG-60H thermal analyzer. The high-resolution mass spectra were obtained with a Waters-LCT-Premier mass spectrometer using 2 ng/μl of sample concentration with a capillary voltage of 2500 V and a desolvation temperature of 250°C using nitrogen gas at 250 L/h, Bruker APEX II CCD area detector diffractometer, with graphite monochromated Mo K3 radiation (50 kV, 30 mA) and temperature of measurement at 173 (2) K coupled with APEX 2 data collection software. Four scans of width 0.5 and 512 × 512 bit data frames were involved for data collection, SAINT + program was used to achieve the data reduction, and face indexed absorption corrections were made using XPRE.