Foxp3yfp cre

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The Foxp3YFP-Cre is a genetically engineered mouse strain that expresses a yellow fluorescent protein (YFP) and the Cre recombinase enzyme under the control of the Foxp3 gene promoter. The Foxp3 gene encodes a transcription factor that is essential for the development and function of regulatory T cells (Tregs). This mouse strain allows for the identification and manipulation of Tregs in vivo.

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Foxp3YFP-cre mice (27 citations) Foxp3YFP (3 citations) B6.Foxp3YFP-cre (2 citations)

43 protocols using foxp3yfp cre

Genetic Mouse Models for Regulatory T Cell Research

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Pdk1-floxed mice (15 (link)) were crossed to Foxp3^YFP-cre (Jackson laboratory). R26-Stop^FLikk2ca mice were purchased from Jackson laboratory and crossed to Foxp3^YFP-cre and Foxp3^YFP-crePdk1^f/f mice.

Oh H., Zhao J., Grinberg-Bleyer Y., Postler T.S., Wang P., Park S.G., Rabadan R., Hayden M.S, & Ghosh S. (2021). PDK1 is required for maintenance of CD4+ FoxP3+ regulatory T cell function. Journal of immunology (Baltimore, Md. : 1950), 206(8), 1776-1783.

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Conditional Knockout of Mitochondrial Proteins

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C57BL/6 Uqcrfs^fl/fl (RISP^fl/fl) are previously described^{8 (link)}. C57BL/6 mice harboring a loxp-flanked exon 1 of the Uqcrq gene (encodes QPC, QPC^fl/fl mice) were generated by Ozgene (locus map in Extended Data Figure 4a). The frt-flanked neomycin resistance cassette was removed by crossing QPC^fl/fl mice to mice expressing FLP-recombinase (FLPo Mice Jackson Lab, Stock no. 011065). Loss of the Neo-cassette was confirmed using PCR. QPC^fl/fl mice were genotyped using the following primers: 1. 5’-CTTCCGCTCCTCCCGGAAGT-3’, 2. 5’-TTCCCAAACTCGCGGCCCATG-3’ and 3. 5’-CAATTCCAGCCAACAGTCCC-3’ which allow identification of the Uqcrq wild-type, loxp-flanked, and excised alleles. RISP^fl/fl and QPC^fl/fl mice were checked for C57BL/6 status and both are >99% C57BL/J using the genome scanning service from Jackson Laboratories. Foxp3^YFP-Cre (stock no. 016959), Foxp3^{eGFP-Cr-eERT2} (stock no. 016961), and ROSA26Sor^CAG-tdTomato (Ai14, stock no. 007908) mice were obtained from Jackson Laboratory. 40 mg/ml tamoxifen dissolved in corn oil was administered to mice every third day using oral gavage to reach an effective dose of 320mg/kg in 8 to 12-week old mice. All animal procedures were approved by Institutional Animal Care and Use Committee (IACUC) at Northwestern University.

Weinberg S.E., Singer B.D., Steinert E.M., Martinez C.A., Mehta M.M., Martínez-Reyes I., Gao P., Helmin K.A., Abdala-Valencia H., Sena L.A., Schumacker P.T., Turka L.A, & Chandel N.S. (2019). Mitochondrial complex III is essential for regulatory T cell suppressive function. Nature, 565(7740), 495-499.

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Generating AMPK-KO Mice for Research

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C57BL/6J, Rag^−/−, AMPKα1^fl/fl, and Foxp3^YFP-Cre mice were purchased from the Jackson Laboratory (Bar Harbor, ME, USA). AMPKα1^fl/fl mice were crossed with Foxp3^YFP-Cre mice to generate AMPK-KO mice. The 6–8- and 24–32-week-old AMPK-KO mice were used as young and aged mice, respectively. Age- and sex-matched littermate Foxp3^yfp-cre mice were used as controls. The other mice were used at 6–10 weeks of age unless otherwise stated. All mice were maintained under specific pathogen-free conditions in the animal facilities of Yeungnam University. All animal experiments were approved by the Institutional Animal Care Committee (IACUC) of Yeungnam University.

Pokhrel R.H., Kang B., Timilshina M, & Chang J.H. (2022). AMPK Amplifies IL2–STAT5 Signaling to Maintain Stability of Regulatory T Cells in Aged Mice. International Journal of Molecular Sciences, 23(20), 12384.

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Mouse Transgenic Models for Immunology

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Foxp3^YFP-Cre (Ref.³²), Foxp3^GFP-Cre-ERT2 (Ref.³³), Rosa26^{STOP f/f-YFP} (Ref.^{34 (link)}), Rosa26^{STOP f/f-IKK2ca} (Ref.^{35 (link)}), Ifng KO,³⁶ B6-scurfy (Ref.^{37 (link)}) and C57BL/6/J mice were purchased from Jackson laboratories. Ramnik J. Xavier and James J. Moon (MGH) provided CARMA1^f/f (Ref.^{38 (link)}) and Rag1 KO mice, respectively. Animals were housed in specific pathogen-free facilities at the Massachusetts General Hospital (MGH) and all experimental studies were approved and performed in accordance with guidelines and regulations implemented by the MGH Institutional Animal Care and Use Committee (IACUC). For survival studies the age of mice at euthanasia mandated by a moribund state of health was recorded in Kaplan Meyer plots.

Pilato M.D., Kim E.Y., Cadilha B.L., Pruessmann J.N., Nasrallah M.N., Seruggia D., Usmani S.M., Misale S., Zappulli V., Carrizosa E., Mani V., Ligorio M., Warner R.D., Medoff B.D., Marangoni F., Villani A.C, & Mempel T.R. (2019). Targeting the CBM complex causes Treg cells to prime tumors for immune checkpoint therapy. Nature, 570(7759), 112-116.

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Murine Models for Regulatory T Cell

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Foxp3^EGFPCre, Foxp3^YFPCre, Notch1^fl/fl, Rosa26^N1c/N1c, Rag1^−/−, CD45.1, Foxp3^EGFP and BALB/c mice were obtained from the Jackson Laboratory ^{28 (link), 30 (link), 48 (link)}. Rictor^fl/fl mice were obtained from the Mutant Mouse Regional Resource Center. Pofut1^fl/fl and Rbpj^fl/fl were kind gifts of Pamela Stanley and Tasuku Honjo, respectively, and were generated as described ^{26 (link), 27 (link)}. All Foxp3 mutant mouse strains and their respective crosses were backcrossed 8–10 generations on C57BL/6 or NOD background where specified. Excepted when it was specified, 8 weeks old mice were used in this study. The mice were housed under specific pathogen-free conditions and used according to the guidelines of the institutional Animal Research Committees at the Boston Children’s Hospital.

Charbonnier L.M., Wang S., Georgiev P., Sefik E, & Chatila T.A. (2015). Control of peripheral tolerance by regulatory T cell-intrinsic Notch signaling. Nature immunology, 16(11), 1162-1173.

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Genetic Manipulation of Murine Models

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All mice used in this study were maintained in SPF or Germ-free facilities at the University of Florida. The mice were littermate controlled and were 6-8 weeks old for experiments unless otherwise noted. Tfam^fl/fl mice were kindly provided by Navdeep Chandel (Northwestern University). Foxp3^Yfp-Cre, Foxp3^{eGFP-Cre-ERT2} mice, CD45.1^homo, Rag1^−/− and ROSA26^Stop-YFP mice were purchased from Jackson Laboratory. Cd4-Cre mice were purchased from Taconic Farms and breed in University of Florida. Experiments were performed with both male and female littermate animals of each genotype unless otherwise indicated. All studies with mice were approved by the Animal Care and Use Committees of the University of Florida.

Fu Z., Ye J., Dean J.W., Bostick J.W., Weinberg S.E., Xiong L., Oliff K.N., Chen Z.E., Avram D., Chandel N.S, & Zhou L. (2019). Requirement of Mitochondrial Transcription Factor A in Tissue-Resident Regulatory T Cell Maintenance and Function. Cell reports, 28(1), 159-171.e4.

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Lkb1 Knockout Treg Cell Mouse Model

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Lkb1^fl/fl, Foxp3^YFPCre, CD45.1⁺, Rag1^−/−, C57BL/6 mice were purchased from Jackson Laboratory. Lkb1^fl/flFoxp3^YFPCre mice were used at age 3–4-wk unless indicated otherwise, with age- and sex-matched WT mice containing the Foxp3^YFPCre allele serving as controls. All mice were maintained at the Salk Institute of Biological Studies specific pathogen-free animal facility in accordance with institutional regulations. All mouse experiments were performed with the approval of the institutional animal care and use committee (IACUC) of the Salk Institute.

He N., Fan W., Henriquez B., Yu R.T., Atkins A.R., Liddle C., Zheng Y., Downes M, & Evans R.M. (2017). Metabolic control of regulatory T cell (Treg) survival and function by Lkb1. Proceedings of the National Academy of Sciences of the United States of America, 114(47), 12542-12547.

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Genetic Manipulation of Regulatory T Cells

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Foxp3^EGFPCre, Foxp3^YFPCre, CD4^Cre,
Rptor^fl/fl, Foxo1^fl/fl, R26^YFP and
Rag₁^–/– mice were obtained from the Jackson Laboratory.
Rictor^fl/fl mice were obtained from the Mutant Mouse Regional Resource Center.
R26^Foxo1AAA and Pfkfb3^fl/fl mice were generated as described
^{33 (link), 41 (link)}.
Foxp3^K276X, Foxp3^EGFP, CD45.1
Foxp3^EGFP, Foxp3^ΔEGFPiCre and their respective
crosses were backcrossed 8–10 generations on C57/BL6/J, excepted
Foxp3^ΔEGFPiCreFoxo1^Δ/Δ and
Foxp3^ΔEGFPiCreRictor^Δ/ΔFoxo1^Δ/Δstrain. A list of all mouse strains used is reported in Supplementary Table
1. Excepted when it was specified, 25–28 days old mice were used in this study. The mice were housed under
specific pathogen-free conditions and used according to the guidelines of the institutional Animal Research Committees at the
Boston Children’s Hospital.

Charbonnier L.M., Cui Y., Victor E., Harb H., Lopez D., Bleesing J.J., Garcia-Lloret M.I., Chen K., Ozen A., Carmeliet P., Li M.O., Pellegrini M, & Chatila T.A. (2019). Functional Reprogramming of Regulatory T cells in the absence of Foxp3. Nature immunology, 20(9), 1208-1219.

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Genetically Modified Mice for Immunological Research

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Foxp3^YFPCre (B6.129(Cg)-Foxp3^{tm4(YFP/icre)Ayr}/J)(44 (link)), Stk3^fl/flStk4^fl/fl (Stk4^tm1.1RjoStk3^tm1.1Rjo/J)(45 (link)) and Wwtr1^fl/flYap1^fl/fl (Wwtr1^tm1HmcYap1^tm1Hmc/WranJ) (46 (link)), Lats1^fl/fl (Lats1^tm1.1Jfm/RjoJ) and lats2^fl/fl (Lats2^tm1.1Jfm/RjoJ) (47 (link)) mice were obtained from the Jackson Laboratory. Foxp3^∆EGFPiCre mice were generated as described (22 (link)). Except when it was specified, 25–28 days old mice were used in this study. The mice were housed under specific pathogen-free conditions and used according to the guidelines of the institutional Animal Research Committees at the Boston Children’s Hospital (protocol 00001278).

Cui Y., Benamar M., Schmitz-Abe K., Poondi-Krishnan V., Chen Q., Jugder B.E., Fatou B., Fong J., Zhong Y., Mehta S., Buyanbat A., Eklioglu B.S., Karabiber E., Baris S., Kiykim A., Keles S., Stephen-Victor E., Angelini C., Charbonnier L.M, & Chatila T.A. (2022). A Stk4 -Foxp3-P65 Transcriptional Complex Promotes Treg Cell Activation and Homeostasis. Science immunology, 7(75), eabl8357.

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Mouse Breeding and Maintenance for Research

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Wild-type C57BL/6 mice were originally purchased from Jackson Laboratories (Bar Harbor, ME), then bred and maintained in the UCSF specific pathogen-free (SPF) facility on the Parnassus campus for use in experiments. Il1r^fl/fl, Il6r^l/fl, Cd4-Cre, CAG::KikGr³³ and Foxp3^YFP-cre mice were purchased from Jackson and bred in-house. A20^fl/fl, Abin^fl/fl, and Villin-Cre^ERT2 mice (Kattah et al., 2018 (link)) were a gift from A. Ma. All animals were 7 days–10 weeks old at the time of experiments. Littermates of the same sex were socially housed under a 12 h light/dark cycle and randomly assigned to experimental groups whenever possible. Animals of both sexes were used and this variable was balanced as a variable across experimental groups. Animal work was performed in accordance with the NIH Guide for the Care and Use of Laboratory Animals and the guidelines of the Laboratory Animal Resource Center and Institutional Animal Care and Use Committee of the University of California, San Francisco.

Merana G.R., Dwyer L.R., Dhariwala M.O., Weckel A., Gonzalez J.R., Okoro J.N., Cohen J.N., Tamaki C.M., Han J., Tasoff P., Palacios-Calderon Y., Ha C.W., Lynch S.V., Segre J.A., Kong H.H., Kattah M.G., Ma A, & Scharschmidt T.C. (2022). Intestinal inflammation alters the antigen-specific immune response to a skin commensal. Cell reports, 39(9), 110891.

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