Irdye 680 conjugated goat anti rabbit

Wogonin and acyclovir were obtained from the National Institutes for Food and Drug Control in China (Beijing, China). SB203580, SP600125, and MG132 were purchased from Beyotime Biotechnology Institute (Haimen, Jiangsu, China). Alexa Fluor 488-conjugated goat anti-mouse IgG (H + L), DAPI, DRAQ5 and SYBR green real-time PCR reagent were obtained from Life Technologies, Thermo Fisher Scientific (Carlsbad, CA, USA). IRDye 680-conjugated goat-anti-rabbit and IRDye 800-conjugated goat-anti-mouse antibodies were obtained from LI-COR (Lincoln, NE, USA). Antibodies specific for HSV-1/2 gD, HSV-1 ICP0, HSV-1 ICP4, HSV-1 ICP27, JNK2, p38, GAPDH, and RIPA lysis buffer were purchased from Santa Cruz (Santa Cruz, CA, USA). p65, p-p38, p-c-Jun, p-JNK1/2, p-ATF-2, and IκB-α antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA). Bright-Glo luciferase assay system was purchased from Promega (Madison, WI, USA).
Vero and HEC-1-A cells were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA). NF-κB-luc and AP-1-luc reporter plasmids were purchased from Clontech (Palo Alto, CA, USA). HSV-1(HF), HSV-1/blue and HSV-2 (G) were propagated and titrated in Vero cells as described previously [36 (link)].