Anti p73 ep436y

Manufactured by Abcam

Anti-p73 (EP436Y) is a rabbit monoclonal antibody that recognizes the p73 protein. p73 is a member of the p53 tumor suppressor family and plays a role in apoptosis, cell cycle regulation, and cellular differentiation. The antibody can be used for applications such as western blotting and immunohistochemistry.

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Lab products found in correlation

Anti p53 c 19, by Santa Cruz Biotechnology (2 mentions) Anti ha y 11, by Santa Cruz Biotechnology (2 mentions)

Spelling variants of this product

Anti-p73 (6 citations) P73 (EP436Y) (4 citations) EP436Y (4 citations)

2 protocols using anti p73 ep436y

In silico prediction and in vitro validation of p73 binding site in AMOT gene

Cited 1 time

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In silico prediction of the p73 responding element (RE) within the human AMOT gene (ENSG00000126016-; Transcript ID ENST 00000371959.7) was performed using the open access database for eukaryotic transcription factor binding profiles JASPAR [39 (link)]. Matrix models MA0861.1 and MA0525.1 were applied, and a site with a score of 13.3 was selected for further in vitro analysis. The following primers encompassing the p73RE in human AMOT gene were used: F→5′-TGTCCCCTTTTCTGCAGAGC-3′ and R→5′-GCTCCCCACTGACACGTTAA-3′.
ChIP analysis was carried out in HA-TAp73β-Saos2-Tet-On cells as described [25 (link)], using the following antibodies: anti-HA (Y-11) (Santa Cruz Biotechnology, #sc805), anti-p73 (EP436Y) (Abcam, # ab40658) and anti-p53 (C-19) (Santa Cruz Biotechnology, #sc1311).

Maeso-Alonso L., Alonso-Olivares H., Martínez-García N., López-Ferreras L., Villoch-Fernández J., Puente-Santamaría L., Colas-Algora N., Fernández-Corona A., Lorenzo-Marcos M.E., Jiménez B., Holmgren L., Wilhelm M., Millan J., del Peso L., Claesson-Welsh L., Marques M.M, & Marin M.C. (2022). p73 is required for vessel integrity controlling endothelial junctional dynamics through Angiomotin. Cellular and Molecular Life Sciences, 79(10), 535.

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In silico Prediction and ChIP Analysis of p73 Binding Site in AMOT Gene

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In silico prediction of the p73 responding element (RE) within the human AMOT gene (ENSG00000126016-;
Transcript ID ENST 00000371959.7) was performed using the open access database for eukaryotic transcription factor binding profiles JASPAR [37] (link). Matrix models MA0861.1 and MA0525.1 were applied, and a site with a score of 13.3 was selected for further in vitro analysis. Primers encompassing the p73RE in human AMOT gene were as follows: F →5'-TGTCCCCTTTTCTGCAGAGC -3´ and R→5'-GCTCCCCACTGACACGTTAA -3´.
ChIP analysis was carried out in HA-TAp73β-Saos2-Tet-On cells as described [25] (link), using the following antibodies: anti-HA (Y-11) (Santa Cruz Biotechnology, #sc805), anti-p73 (EP436Y) (Abcam, # ab40658) and anti-p53 (C-19) (Santa Cruz Biotechnology, #sc1311).

Maeso‐Alonso L., Alonso-Olivares H., Martínez‐García N., López‐Ferreras L., Villoch-Fernández J., Puente-Santamaría L., Colás-Algora N., Fernández‐Corona A., Lorenzo-Marcos M.E., Jiménez B., Holmgren L., Wilhelm M., Millán J., Peso L.d., Claesson‐Welsh L., Marqués M.M., & Marín M.C. (2022). p73 is required for vessel integrity controlling endothelial junctional dynamics through Angiomotin.

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