Milk samples (n = 3) were identified as being of interest for additional analysis, based on the results of 16S rRNA gene amplicon sequencing, because of a microbiota highly dominated by a single taxonomic group with the low SCC (< 200,000 cells/mL). These samples were further analyzed via shotgun metagenomics. Sequencing libraries were prepared with Nextera XT DNA Flex Library Preparation Kit (Illumina Inc., USA) and Nextera XT Index Kit (Illumina Inc., USA) according to the manufacturer’s instructions. Paired-end sequencing (2 × 150 bp) was performed on a NovaSeq 6000 machine (Illumina Inc., USA) at Genome Quebec (Montreal, Canada).
Nextera xt dna flex library preparation kit
Manufactured by Illumina
Sourced in United States
The Nextera XT DNA Flex Library Preparation Kit is a laboratory equipment product manufactured by Illumina. The kit is designed for the preparation of DNA libraries for next-generation sequencing. It provides a streamlined workflow for library construction, enabling efficient sample preparation for sequencing.
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Lab products found in correlation
3 protocols using nextera xt dna flex library preparation kit
1
Shotgun Metagenomic Analysis of Milk Microbiome
2
Shotgun Metagenomic Analysis of Milk Microbiome
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Milk samples (n = 3) were identified as being of interest for additional analysis, based on the results of 16S rRNA gene amplicon sequencing, because of a microbiota highly dominated by a single taxonomic group with the low SCC (< 200,000 cells/mL). These samples were further analyzed via shotgun metagenomics. Sequencing libraries were prepared with Nextera XT DNA Flex Library Preparation Kit (Illumina Inc., USA) and Nextera XT Index Kit (Illumina Inc., USA) according to the manufacturer’s instructions. Paired-end sequencing (2 × 150 bp) was performed on a NovaSeq 6000 machine (Illumina Inc., USA) at Genome Quebec (Montreal, Canada).
3
Shotgun Metagenomic Analysis of Milk Microbiome
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Milk samples (n = 3) were identified as being of interest for additional analysis, based on the results of 16S rRNA gene amplicon sequencing, because of a microbiota highly dominated by a single taxonomic group with the low SCC (< 200,000 cells/mL). These samples were further analyzed via shotgun metagenomics. Sequencing libraries were prepared with Nextera XT DNA Flex Library Preparation Kit (Illumina Inc., USA) and Nextera XT Index Kit (Illumina Inc., USA) according to the manufacturer’s instructions. Paired-end sequencing (2 × 150 bp) was performed on a NovaSeq 6000 machine (Illumina Inc., USA) at Genome Quebec (Montreal, Canada).
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