Antibodies and their sources were: anti-HA (Roche, 15645900), anti-Cdc48 (own production), anti-α-tubulin (Abcam, YL1/2 MA1-80017), anti-Pma1 (Abcam, 40B7 Ab4645), anti-RGS-His (Qiagen, 34610), anti-Vinculin (Sigma, hVIN1 V9264), anti-GAPDH (Cell signalling technology, 14C10 2118), anti-Na/K ATPase α-1 (Merck, C464.6 05–369), anti-Hsp105 (HSPH1, Abcam, Ab108625), anti-HSPA4 (Abcam, Ab185219), anti-Ubiquitin (Dako, Z0458), anti-Myc (Chromotek, 9E1 9e1-100), anti-Rpn1 (Enzo Life Sciences, p112-1 PW9270), anti-20S α-subunits (Enzo Life Sciences, MCP231 PW8195), anti-Hsp70 (Invitrogen, 5A5 MA3-007), anti-Aspartoacylase (Thermo Scientific, PA5-29180), anti-GFP (Chromotek, 3H9 3h9-100). Secondary antibodies and their sources were: HRP-anti-rat (Invitrogen, 31470), HRP-anti-mouse (Dako, P0260), HRP-anti-rabbit (Dako, P0448).
Hrp anti mouse
The HRP anti-mouse is a laboratory reagent used for the detection and quantification of mouse-derived proteins in various analytical applications. It consists of horseradish peroxidase (HRP) conjugated to an antibody that specifically binds to mouse immunoglobulins. This product can be used in techniques such as Western blotting, ELISA, and immunohistochemistry to visualize and measure the presence of mouse proteins in samples.
Lab products found in correlation
6 protocols using hrp anti mouse
Western Blot Analysis of Cellular Proteins
Antibodies and their sources were: anti-HA (Roche, 15645900), anti-Cdc48 (own production), anti-α-tubulin (Abcam, YL1/2 MA1-80017), anti-Pma1 (Abcam, 40B7 Ab4645), anti-RGS-His (Qiagen, 34610), anti-Vinculin (Sigma, hVIN1 V9264), anti-GAPDH (Cell signalling technology, 14C10 2118), anti-Na/K ATPase α-1 (Merck, C464.6 05–369), anti-Hsp105 (HSPH1, Abcam, Ab108625), anti-HSPA4 (Abcam, Ab185219), anti-Ubiquitin (Dako, Z0458), anti-Myc (Chromotek, 9E1 9e1-100), anti-Rpn1 (Enzo Life Sciences, p112-1 PW9270), anti-20S α-subunits (Enzo Life Sciences, MCP231 PW8195), anti-Hsp70 (Invitrogen, 5A5 MA3-007), anti-Aspartoacylase (Thermo Scientific, PA5-29180), anti-GFP (Chromotek, 3H9 3h9-100). Secondary antibodies and their sources were: HRP-anti-rat (Invitrogen, 31470), HRP-anti-mouse (Dako, P0260), HRP-anti-rabbit (Dako, P0448).
Immunohistochemical Analysis of Myostatin
Quantification of LRP-1 in Neutrophils
Immunohistochemical Analysis of Tissue Samples
and embedded in paraffin. Hematoxylin and eosin staining was performed using
standard methods and tissue specimens from experimental animals were reviewed in
a blinded fashion by a clinical pathologist (M. M. I.) For Ki67 staining,
3–4 micron tissue sections were cut from paraffin blocks and baked
overnight in a dry slide incubator, then deparaffinized on a Shandon-Lipshaw
Varistain using a series of incubations in xylene, ethanol, then water. Antigen
retrieval was achieved by incubating slides in Tris-HCL 9.0 AR buffer in a T-FAL
OPTIMA pressure cooker. Slides were rinsed, then endogenous peroxidase was
blocked by immersing slides in 3% hydrogen peroxide for 5 minutes. Following
washes in nanopure water and TBS-20, primary antibody was applied at a dilution
of 1:200 (Ki67, MIB-1 Clone, Dako). Following primary antibody incubation,
slides were washed and then incubated with envision-labelled polymer-HRP
Anti-Mouse (Dako). Slides were washed, then DAB+ solution (DakoCytomation) was
added for a 15-minute incubation, after which slides were rinsed with nanopure
water. Chromogen signal was enhanced using DAB Sparkle Enhancer (Biocare).
Slides were washed, then counterstained with Harris Hematoxylin, dehydrated,
cleared, then mounted using Cytoseal (VWR).
Immunohistochemical Analysis of Tissue Samples
Protein Extraction and Western Blot
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