Proleukin human il 2

Manufactured by Thermo Fisher Scientific

Proleukin is a recombinant human interleukin-2 (IL-2) product. IL-2 is a cytokine that plays a crucial role in the activation and proliferation of T cells, a type of immune cell. Proleukin is used for research purposes to study the function and regulation of the immune system.

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Human IL-2 (145 citations) IL-2 (Proleukin) (2 citations)

4 protocols using proleukin human il 2

Generation of Lymphokine-activated Killer Cells

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Lymphokine-activated killer cells (LAKs) were generated from Rag1^−/−bone marrow as described previously (Hunter et al., 1997 ; Wherry et al., 1991 (link)). Briefly, whole bone marrow was plated at 1 M cells/ml in cRPMI +400 U/ml Proleukin human IL-2 (Peprotech). Fresh IL-2 was added every third day, and cells were used for experiments between days 7 and 10.

Clark J.T., Christian D.A., Gullicksrud J.A., Perry J.A., Park J., Jacquet M., Tarrant J.C., Radaelli E., Silver J, & Hunter C.A. (2021). IL-33 promotes innate lymphoid cell-dependent IFN-γ production required for innate immunity to Toxoplasma gondii. eLife, 10, e65614.

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Generating Lymphokine Activated Killer Cells

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Lymphokine Activated Killer cells (LAKs) were generated from
Rag1^−/− bone marrow as
described previously.^{72 (link),73 (link)} Briefly, whole bone marrow
was plated at 1M cells/mL in cRPMI +400U/mL Proleukin human IL-2
(Peprotech). Fresh IL-2 was added every third day, and cells were used for
experiments between days 7–10.

Clark J.T., Weizman O.E., Aldridge D.L., Shallberg L.A., Eberhard J., Lanzar Z., Wasche D., Huck J.D., Zhou T., Ring A.M, & Hunter C.A. (2023). IL-18BP mediates the balance between protective and pathological immune responses to Toxoplasma gondii. Cell reports, 42(3), 112147.

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Generating Lymphokine Activated Killer Cells

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Naora H., Yang Y., Montz F.J., Seidman J.D., Kurman R.J, & Roden R.B. (2001). A serologically identified tumor antigen encoded by a homeobox gene promotes growth of ovarian epithelial cells. Proceedings of the National Academy of Sciences of the United States of America, 98(7), 4060-4065.

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Generating Lymphokine Activated Killer Cells

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Lymphokine Activated Killer cells (LAKs) were generated from Rag1 -/-bone marrow as described previously [73] , [74] . Briefly, whole bone marrow was plated at 1M cells/mL in cRPMI + 400U/mL Proleukin human IL-2 (Peprotech). Fresh IL-2 was added every 3 rd day, and cells were used for experiments between days 7 to 10.

Clark J.T., Christian D.A., Gullicksrud J.A., Perry J.A., Park J., Jacquet M., Tarrant J.C., Radaelli E., Silver J.S., & Hunter C.A. (2021). IL-33 promotes innate lymphoid cell-dependent IFN-γ production required for innate immunity toToxoplasma gondii.

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