Methylene blue

Manufactured by Maclin Biochemical Technology

Sourced in China

Methylene blue is a versatile laboratory chemical that serves as an oxidation-reduction indicator. It is a dark blue crystalline solid that readily dissolves in water, producing a blue-colored solution. Methylene blue has various applications in analytical chemistry, biological staining, and industrial processes, where its distinct color change properties are utilized.

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Lab products found in correlation

28 protocols using methylene blue

Plasma-Assisted Centrifugal Spinning of Polymer Fibers

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CA with a molecular weight of 100,000 g/mol was supplied from Acros Organics (Fisher Scientific Worldwide (Shanghai) Co. Ltd., Shanghai, China). Toluene (analytically pure, Sinopharm Chemical Reagent Co. Ltd., (Shanghai, China). D4 (90%, CAS#556-67-2) was purchased from Zhejiang Quzhou Jun Shun Silicone Co. Ltd., (Quzhou, China). Acetone (analytically pure, Zhejiang Sanying Chemical Reagent Co. Ltd., Hangzhou, China). N-hexane (analytically pure, Hangzhou Gaojing Fine Chemical Co. Ltd., Hangzhou, China). 1, 2-dibromoethane, methylene blue, Disperse Red FB and Span 80 were of analytical grade and purchased from Shanghai McLean biochemical technology Co. Ltd., (Hangzhou, China). Oxygen (99.9%) was supplied by Hangzhou Electrochemical Group Co. Ltd., (Hangzhou, China).
The low-temperature plasma was produced by a conventional radio frequency (RF) capacitance coupling plasma device (Model: HD-1A, Changzhou Changtai Plasma Technology Development Ltd., Changzhou, China). The centrifugal spinning equipment is shown in Figure 1. The spinning head is fixed on the motor shaft by screws, the spinneret is located on the sidewall of the spinning head, and the receiving rod is used to receive the fibers.

Yu X., Zhang X., Xing Y., Zhang H., Jiang W., Zhou K, & Li Y. (2021). Development of Janus Cellulose Acetate Fiber (CA) Membranes for Highly Efficient Oil–Water Separation. Materials, 14(20), 5916.

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Seaweed-Coconut Adsorption for Methylene Blue Removal

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The seaweed was collected from Guilin Ocean Beach, Haikou. The coconut shell and coconut coat were prepared by separating edible coconut. The phosphoric acid and potassium hydroxide were supplied from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Methylene blue was supplied from Shanghai McLean Biochemical Technology Co., Ltd. (Shanghai, China). Hydrochloric acid was supplied from Xilong Science Company (Shanghai, China).

Xu J., Fu M., Ma Q., Zhang X., You C., Shi Z., Lin Q., Wang X, & Feng W. (2023). Modification of biochar by phosphoric acid via wet pyrolysis and using it for adsorption of methylene blue. RSC Advances, 13(22), 15327-15333.

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Enzyme-Crosslinked Hydrogel for Drug Delivery

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1-ethyl-3-(3’-dimethylaminopropyl) carbodiimide (EDC), N-hydroxysuccinimide (NHS), Glucose oxidase (GOx) from Aspergillus niger were purchased from Sigma-Aldrich (St. Louis, MO, USA). Poly (ethylene glycol) (SH-PEG-COOH, Mw = 2000) was purchased from JenKem Technology Co., Ltd (Beijing, China). RGD peptide (Arg-Gly-Asp, purity > 97%) was obtained from GL Bio-Chem Co., Ltd (Shanghai, China). Calcium chloride (CaCl₂), copper chloride (CuCl₂), L-ascorbic acid (AA), Methylene blue (MB), and Metformin hydrochloride (MET) were purchased from Macklin Biochemical Co., Ltd (Shanghai, China). All other reagents and chemicals were commercially purchased at highest grade available and used as received.

Zhang Y., Hu H., Deng X., Song Q., Xing X., Liu W, & Zhang Y. (2022). Cascade-Enhanced Catalytic Nanocomposite with Glutathione Depletion and Respiration Inhibition for Effective Starving-Chemodynamic Therapy Against Hypoxic Tumor. International Journal of Nanomedicine, 17, 5491-5510.

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Synthesis and Characterization of Fluorocarbon Surfactant

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The fluorocarbon surfactant C4-Br was synthesized using the method reported in our another work^{27 (link)}. Sodium hexanesulfonate and methylene blue were provided by Shanghai Macklin Biochemical Co., Ltd. Cyclohexane and n-heptane produced by Chengdu Cologne Chemical co., Ltd. Diesel oil 0# and aviation kerosene were provided by China National Aviation Fuel Grope. Solvent naphtha purchased from Hubei Xin Bonus Chemical Co., Ltd. All reagents were used without further purification. All aqueous solutions were prepared using deionized water.
Tensiometer (model A601) was produced by USA Kino Industry Co., Ltd. The oil pan (fire tray) was made of stainless steel by ourselves according to international standards⁸. High-definition camera (model D810) made by Nikon Corporation. Flammable gas detector (model MS400) was produced by Beijing Sipute Technology Co., Ltd. Kinematic viscosimeter (model SYD-265C) was provided by Shanghai Changji Geological Instrument Co., Ltd. Capillary-stoppered pyknometer (model 25 mL) was produced by Fuzhou Beibo Experimental Instrument Co., Ltd. Thermogravimetric analyzer (Perkin-Elmer TGA 4000) was made by PerkinElmer Co., Ltd..

Jia X., Luo Y., Huang R., Zhu X., Zhang Y, & Liu Q. (2021). Two-dimensional spreading properties and sealing characteristics of fluorocarbon surfactants on several typical hydrocarbon fuels. Scientific Reports, 11, 1148.

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Silk Fibroin Adsorption Characterization

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The raw silkworm cocoon was directly obtained from sericulturists of Sichuan, China. Potassium hydroxide (KOH) were purchased from Aladdin Chemistry Co. Ethanol absolute and hydrochloric acid (37 wt%, HCl) were purchased from Sinopharm Chemical Reagent Co., Ltd. Methylene blue (MB) was purchased from Shanghai Macklin Biochemical Co., Ltd. All the chemicals used in the sample preparation were used as received without further purification. Furthermore, deionized water was used to prepare different concentrations of MB solutions.

Zhu G., Liu Q., Cao F., Qin Q, & Jiao M. (2019). Silkworm cocoon derived N, O-codoped hierarchical porous carbon with ultrahigh specific surface area for efficient capture of methylene blue with exceptionally high uptake: kinetics, isotherm, and thermodynamics. RSC Advances, 9(58), 33872-33882.

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Synthesis and Characterization of Polymeric Membranes

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PAN polymer was purchased from Dechao Chemical Co. Ltd. (Hubei, China). Sodium sulfate (Na₂SO₄), sodium hydroxide (NaOH), magnesium chloride (MgCl₂), calcium chloride (CaCl₂), sodium chloride (NaCl), sodium hydrogen phosphate (Na₂HPO₄), nickel sulfate (NiSO₄), magnesium sulfate (MgSO₄), N,N-dimethylformamide (DMF), and n-hexane were purchased from Sinopharm Chemical Reagent Co. Ltd. (Shanghai, China). Methyl orange, CR, AB93, methylene blue, crystal violet, and rhodamine B were provided by Macklin Biochemical Co. Ltd. (Shanghai, China). TC, CLP, and BAC were obtained from Sinopharm Chemical Reagent Co. Ltd. (Shanghai, China). TMC, BSA, and HA were purchased from Aladdin Reagent Co. Ltd. (Shanghai, China). St was purchased from Nuoyi Biotechnology Co. Ltd. (Zhejiang, China). Polyethylene glycols (PEGs; 200, 400, 600, 1000, 1500, 2000 and 4000 Da) were obtained from Macklin Biochemical Co. Ltd. (Shanghai, China) and used to evaluate MWCO of membranes. The water used in all experiments was deionized water. All chemicals were used as received without further purification.

Bai Y., Liu B., Li J., Li M., Yao Z., Dong L., Rao D., Zhang P., Cao X., Villalobos L.F., Zhang C., An Q.F, & Elimelech M. (2023). Microstructure optimization of bioderived polyester nanofilms for antibiotic desalination via nanofiltration. Science Advances, 9(18), eadg6134.

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Tree Shrew Spermatogonial Stem Cell Transplantation

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Recipient tree shrew preparation and cell transplantation were performed following previous research (Bi et al., 2021a , b; Li et al., 2017 (link)). In total, 15 one-year-old tree shrews were used as recipients. Briefly, undifferentiated SPGs were infected with lentiviral simian immunodeficiency virus (SIV) expressing GAE-CAG-EGFP/WPRE (SIV-EGFP). The EGFP-expressing cells were collected using FACS. The enriched EGFP⁺ cells were further cultured and the expression of EGFP proteins was validated by immunoblot analysis. For transplantation, the EGFP-expressing cells (5×10⁵–6×10⁵) were mixed with methylene blue (Macklin, Lot# C11748711, China), and transplanted into the recipient seminiferous tubules of the testes through the efferent ducts. Transplantation efficiency was monitored using methylene blue. The cells (3×10⁵–4×10⁵) were transplanted into the recipient testes. In total, three tree shrew SSC lines were used to assess spermatogenic capacity.

Li C., Bi R., Wang L., Ma Y.H., Yao Y.G, & Zheng P. (2023). Characterization of long-term ex vivo expansion of tree shrew spermatogonial stem cells. Zoological Research, 44(6), 1080-1094.

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Synthesis and Purification of Chemical Reagents

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Zinc acetate dihydrate, manganese chloride tetrahydrate, urea, glucose, ethanol, isopropyl alcohol, sodium oxalate, NaOH, and HCl were obtained from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Methyl orange, methylene blue, bisphenol A, and benzoquinone were acquired from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). Congo red and malachite green were purchased from Aladdin Shanghai Co., Ltd. (Shanghai, China).
All chemicals were of p.a. grade and were utilized without additional purification.

Liang H., Zhao Y., Liu T., Li R., Li R., Zhu Y, & Fang F. (2024). Zn-Doped MnCO3/CS Composite Photocatalyst for Visible-Light-Driven Decomposition of Organic Pollutants. Molecules, 29(5), 1094.

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Fabric Modification with Titanate

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Cotton fabrics (plain weave, 134 g m⁻²) were bought from the Hongda weaving factory (Hebei, China). Tetrabutyl titanate, absolute ethanol, HNO₃, urea, NaOH, methylene blue (MB) and acid red (AR), were purchased from Macklin Biochemical Technology Co., Ltd (Shanghai, China). Absorbent cotton was purchased from Hualu Sanitary Materials Co., Ltd (Shangdong, China). All solvents and chemicals were analytically pure and did not require further treatment.

Chen W., Feng X., Zhang D., Lu F., Wang H., Tan J., Xu Q., Liu Y., Cao Z, & Su X. (2022). In situ synthesis of TiO2/NC on cotton fibers with antibacterial properties and recyclable photocatalytic degradation of dyes. RSC Advances, 12(31), 19974-19980.

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Synthesis and Characterization of PS/PA6 Blends

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Polystyrene (PS, Mw, 228,800 g/mol) and polyamide (PA6, Mw, 49,400 g/mol) were purchased from Yangzi-BASF Styrenics Company (Hong Kong, China) and UBE Nylon Ltd. (Taphong, Thailand), respectively. Styrene (St), benzoyl peroxide (BPO), toluene, methanol, dichloromethane (CH₂Cl₂), chloroform (CHCl₃), Karl Fischer reagent (without pyridine), silicon oil and paraffin wax were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China) Sunflower oil was purchased from Luhua Group Co., Ltd. (Shanghai China) 3-Isopropenyl-α,α’-dimethylbenzene isocyanate (TMI) and trifluoroacetic anhydride (TFAA) were purchased from Aladdin Co., Ltd. (Fukuoka, Japan) Carbon nanotubes (CNT) were purchased from Chengdu Organic Chemicals Co. Ltd. (Chengdu, China) Methylene blue and oil red O were purchased from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China) PA6 was used after dried at 80 °C in vacuum, and all other chemicals were used as received.

Tang Y., Zhu T., Huang Z., Tang Z., Feng L., Zhang H., Li D., Xie Y, & Zhu C. (2022). Preparation of Nanofiber Bundles via Electrospinning Immiscible Polymer Blend for Oil/Water Separation and Air Filtration. Polymers, 14(21), 4722.

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