Transfected cells in 96-well plates were subjected to 10 μl CCK-8 (TransGen Biotech, Beijing, China). Cell proliferation was assessed through measuring the OD value at 450 nm via microplate spectrophotometer (Bio-Tek, Winooski, VT, USA).
Cck 8
Manufactured by Transgene
Sourced in China
The CCK-8 (Cell Counting Kit-8) is a colorimetric assay for the determination of cell viability and cytotoxicity. It utilizes the tetrazolium salt WST-8 which is reduced by dehydrogenases in living cells to produce a water-soluble formazan dye. The amount of formazan dye generated is directly proportional to the number of living cells, allowing for the quantification of cell proliferation and cytotoxicity.
Automatically generated - may contain errors
Lab products found in correlation
Cell counting kit 8 (cck8), by Transgene (3 mentions)
Microplate reader, by Agilent Technologies (2 mentions)
Microplate spectrophotometer, by Agilent Technologies (1 mentions)
Multifunctional enzyme marking instrument, by Agilent Technologies (1 mentions)
Sulforhodamine b assay, by Merck Group (1 mentions)
Synergy ht multi mode microplate reader, by Agilent Technologies (1 mentions)
Enzyme marker, by Thermo Fisher Scientific (1 mentions)
Automatic microplate reader, by Bio-Rad (1 mentions)
5 ethynyl 20 deoxyuridine edu assay kit, by RiboBio (1 mentions)
Cck 8, by Bio-Rad (1 mentions)
Enspire multilabel reader, by PerkinElmer (1 mentions)
Compound c, by Selleck Chemicals (1 mentions)
Cck 8, by Agilent Technologies (1 mentions)
Compusyn software, by ComboSyn (1 mentions)
Synergy h1m, by Synergy Software (1 mentions)
Cck 8 reagent, by Transgene (1 mentions)
Edu assay kit, by RiboBio (1 mentions)
Microplate reader, by Bio-Rad (1 mentions)
Spectra max i3x multifunctional microplate detection system, by Molecular Devices (1 mentions)
Spectramax i3x, by Molecular Devices (1 mentions)
28 protocols using cck 8
1
Cell Proliferation Assay via CCK-8
2
Cell Viability Assessment of Myoblasts
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The CCK-8 assay was performed to investigate the viability of myoblasts cultured in 96-well plates. Briefly, 100 μl of CCK-8 (TransGen Biotech, Beijing, CN) solution at a 1:10 dilution was added to each well of the plate, and myoblasts were incubated at 37°C for 2.5 h. Absorbance was measured at 450 nm with a microplate reader (Bio-Tek, Winooski, VT). The mean optical density (OD) of six wells for each indicated group was used to calculate the cell viability percentage.
3
Cell Proliferation Assay with DOX
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The cells were diluted in the culture media with or without DOX (Sigma) at a concentration of 2 × 104 cells/mL. Then 200 μL diluted cells were seeded into each well of a 96‐well plate. Cell proliferation was then determined by sulforhodamine B assay13 (Sigma‐Aldrich) or with the CCK‐8 (TransGen Biotech) assay as instructed by the manufacturer. The absorbance was determined at 510 or 450 nm with the multifunctional enzyme marking instrument (Bio‐Tek) after another 0, 24, 48, or 72 hours. The “0 hour” was the time when all seeded cells adhered to the plate.
4
Doxorubicin Cytotoxicity Assay in J774A.1 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
J774A.1 cells at a density of 103 were incubated with Dox (0–20 µg/ml) for 24 and 48 h at 37°C in a 96-well cell culture plate, followed by the addition of 10 μl/well CCK-8 (TransGen Biotech, China). After incubated for 2 h at 37°C, the optical density of the samples at 450 nm was measured.
5
Aikejia Cytotoxicity in CT-26 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
CT-26.WT cells in the logarithmic growth phase were seeded into 96-well plates at concentrations of 10000 cells/well. After 24 h, the cells were respectively cultured in 100 μL complete RPMI 1640 medium in the presence of 0, 6.25, 12.5, 25 and 50 µg/mL Aikejia for 48h. After treatment, 10 μL CCK8 (TransGen Biotech, Beijing, China) was added into each well, and the plates were incubated for 1 h at 37°C. Then, the absorbance was measured at 450 nm using Synergy HT Multi-Mode Microplate Reader (BioTek, Winooski, VT, USA). Statistical results were obtained from three independent experiments.
6
Cell Viability Evaluation via CCK-8
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For CCK-8 analysis (Transgen, China), post-transfection pellet cells were inoculated into 96-well plates (5 × 103 cells per well). At 24, 48, and 72 h, 10 μL of CCK-8 reagent was added to each well and then cultured for 3 h. All experiments were performed in triplicate. The absorbance of each well was then measured at 450 nm using an enzyme marker (Thermo Fisher, USA).
7
Evaluating GZMK Overexpression on Cell Proliferation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
We compared the cell proliferation between GZMK overexpression infected group and negative control vector group by cell counting kit (CCK-8) assay (Transgen, Beijing, China). 3×103 cells were seeded in per well in a 96-well plate, at the end time of 0 h, 24 h, 48 h, and 72 h, 10 μl of CCK-8 solution was added to each well and incubated with 2 hours. The reader machine (Bio-Rad, Hercules, CA, USA) was then used to assess the absorbance at 450 nm. Colony formation assay was also employed to evaluate cell proliferation affected by GZMK overexpression. 2000 cells/well infected by GZMK vector or NC vector were pre-seeded in 6 cm dishes and incubated for ten days. At the end of the assay, 0.1% crystal violet was added to stain the cells, and then measured the absorbance of 550 nm using a microplate reader.
8
Evaluating Cell Proliferation with CCK-8 and EdU Assays
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell Counting Kit-8, CCK-8 (TransGen, Beijing, China) and 5-ethynyl-20-deoxyuridine (EdU) assay kit (Ribobio, Guangzhou, China) were used for cell proliferation according to the manufacturer's instructions. For CCK-8 assay, the stably transfected cells of miR-OIP5 and miR-NC group were seeded in a 96-well plates, and cultured in normal medium. At 24, 48 or 72 h, 96h, 10 μl CCK-8 reagent was added to each well and cultured for 1 h. The absorbance at 450 nm was detected by automatic microplate reader (Bio-Rad, Hercules, CA, USA).
For EdU assay, stably transfected cells were seeded in a 12-well flat-bottomed plate, and incubated with 100μl of 50μM EdU per well for 2h. Then, the cells were fixed with 4% paraformaldehyde for 15 min at room temperature. After removing the buffer, the cells were incubated with 200μl of 2mg/ml glycine for 5min followed by washing with 200μl of PBS. The cells in each well were then treated with 0. 5% Triton X-100 for 20 min at room temperature for permeabilization. After washing with PBS three times, cells were added with 200μl of 1X Apollo solution for 30 min at room temperature in the dark. After that, cells were incubated with 200μl of Hoechst33342 for 30 min at room temperature in the dark followed by washing with PBS. The cells were then observed using fluorescence microscopy. All experiments were performed three times.
For EdU assay, stably transfected cells were seeded in a 12-well flat-bottomed plate, and incubated with 100μl of 50μM EdU per well for 2h. Then, the cells were fixed with 4% paraformaldehyde for 15 min at room temperature. After removing the buffer, the cells were incubated with 200μl of 2mg/ml glycine for 5min followed by washing with 200μl of PBS. The cells in each well were then treated with 0. 5% Triton X-100 for 20 min at room temperature for permeabilization. After washing with PBS three times, cells were added with 200μl of 1X Apollo solution for 30 min at room temperature in the dark. After that, cells were incubated with 200μl of Hoechst33342 for 30 min at room temperature in the dark followed by washing with PBS. The cells were then observed using fluorescence microscopy. All experiments were performed three times.
9
Cytotoxicity Assay of LPS on HUVECs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The cytotoxicity was analyzed with a staining method. HUVEC cells (1 × 104 cells/well) were seeded in a 96-well plate and treated with various concentrations of LPS for 24 h. Then, the culture supernatants were discarded, and the cells were incubated for 2 h with 10% CCK-8 (TransGen, Beijing, China) solution diluted with the culture medium. The absorbance intensity was measured at 450 nm using an Enspire Multilabel Reader (PerkinElmer, United States).
10
Cell Viability Assay with CCK8
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Taking out a 96-well plate once every 24 h, added 10 μL CCK8 (Transgen) solution to each of the test sample, the 96-well plate at 37 °C and 5% CO2 in the condition of incubation 1.5 h. OD-value was measured with the microplate reader, set the wavelength to 450 nm, OD-value of each well was measured, and the date was collected and analyzed. The experiment was conducted for at least three times.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!