At 48 hours after folic acid injection, the mice were placed in metabolic cages to collect 24-hour urine samples for measuring urinary albumin and creatinine excretion. Urinary albumin excretion was measured using Albuwell M ELISA kit (Exocell Inc., Philadelphia, PA, USA), and urinary creatinine excretion was measured using a creatinine ELISA kit (Exocell Inc.). Urinary albumin excretion was expressed as the urinary albumin to creatinine ratio (UACR).
Creatinine elisa kit
Manufactured by Exocell
Sourced in United States, Japan
The Creatinine ELISA kit is a laboratory tool used to quantitatively measure the levels of creatinine in biological samples. It is designed to provide accurate and reliable results for research and diagnostic applications.
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2 protocols using creatinine elisa kit
1
Urinary Albumin and Creatinine Measurement
2
Maternal Blood Pressure and Proteinuria
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Blood pressure during pregnancy was non-invasively measured on GD 14, 17, and 20 using an automatic blood pressure analyzer with a tail-cuff device (BP-98AL; Softron, Tokyo, Japan) after the rats received pre-training for blood pressure measurements once on GD 13 to acclimate the rats to the experimental equipment. The mean value of five readings for each rat was recorded after the rats were placed in a heating chamber maintained at 37 °C for approximately 5–10 min. Urinary samples were collected on GDs 14, 17, and 20, and urinary protein levels (protein/Cre) were measured using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Tokyo, Japan) and Creatinine ELISA kit (Exocell, Newtown Square, Pennsylvania) according to the manufacturer's instructions.
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