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Thbs1

Manufactured by LifeSpan BioSciences
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Thbs1 is a protein that plays a role in cell-to-cell and cell-to-matrix interactions. It is involved in various biological processes, including cell adhesion, angiogenesis, and immune response. Thbs1 is commonly used in research applications to study these cellular mechanisms.

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Lab products found in correlation

Serpine1, by Abcam (2 mentions) Pai 1, by Abcam (2 mentions) Timp 1, by R&D Systems (2 mentions)

2 protocols using thbs1

1

Plasma Protein Quantification by ELISA

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Platelet poor plasma from each treatment group was analyzed by ELISA according to the manufacturer’s instructions. Thbs1 was from LifeSpan Biosciences, SerpinE1 (PAI-1) was from Abcam, uPA was from Innovative Research, and Timp1 was from R&D Systems. Plasma samples were diluted with Assay Buffers before analysis (Thbs1 = 1:5 dilution, PAI-1 = 1:5 dilution, uPA = 1:2 dilution, Timp1 = 1:2 dilution). Thrombopoeitin (Tpo) was measured by ELISA (R&D Systems) on undiluted EDTA-treated plasma according to the manufacturer’s instructions.
Wiley C.D., Liu S., Limbad C., Zawadzka A.M., Beck J., Demaria M., Artwood R., Alimirah F., Lopez-Dominguez J.A., Kuehnemann C., Danielson S.R., Basisty N., Kasler H.G., Oron T.R., Desprez P.Y., Mooney S.D., Gibson B.W., Schilling B., Campisi J, & Kapahi P. (2019). SILAC Analysis Reveals Increased Secretion of Hemostasis-Related Factors by Senescent Cells. Cell reports, 28(13), 3329-3337.e5.
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2

Plasma Protein Quantification by ELISA

Check if the same lab product or an alternative is used in the 5 most similar protocols
Platelet poor plasma from each treatment group was analyzed by ELISA according to the manufacturer’s instructions. Thbs1 was from LifeSpan Biosciences, SerpinE1 (PAI-1) was from Abcam, uPA was from Innovative Research, and Timp1 was from R&D Systems. Plasma samples were diluted with Assay Buffers before analysis (Thbs1 = 1:5 dilution, PAI-1 = 1:5 dilution, uPA = 1:2 dilution, Timp1 = 1:2 dilution). Thrombopoeitin (Tpo) was measured by ELISA (R&D Systems) on undiluted EDTA-treated plasma according to the manufacturer’s instructions.
Wiley C.D., Liu S., Limbad C., Zawadzka A.M., Beck J., Demaria M., Artwood R., Alimirah F., Lopez-Dominguez J.A., Kuehnemann C., Danielson S.R., Basisty N., Kasler H.G., Oron T.R., Desprez P.Y., Mooney S.D., Gibson B.W., Schilling B., Campisi J, & Kapahi P. (2019). SILAC Analysis Reveals Increased Secretion of Hemostasis-Related Factors by Senescent Cells. Cell reports, 28(13), 3329-3337.e5.
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