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1 2 dioleoyloxy 3 trimethylammoniumpropane dotap chloride

Manufactured by Merck Group
Sourced in Switzerland

1,2-dioleoyloxy-3-trimethylammoniumpropane (DOTAP) chloride is a cationic lipid commonly used in laboratory settings. Its core function is to facilitate the delivery of nucleic acids, such as plasmid DNA or mRNA, into cells during transfection experiments.

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2 protocols using 1 2 dioleoyloxy 3 trimethylammoniumpropane dotap chloride

1

DOTAP-DOPE Lipid Nanoparticle Assembly

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For all experiments, the lipids 1,2-dioleoyloxy-3-trimethylammoniumpropane (DOTAP) chloride, (Mw: 698.54 g/mol) and 1.2 dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) were obtained from Sigma Aldrich (Buchs, Switzerland), whereas dipalmitoylphosphatidylcholine (DPPC) was purchased from Avanti Polar Lipids (Alabaster, AL, USA). Methanol and isopropanol were purchased from Fisher Scientific (Loughborough, UK), Chloroform Emprove® from Merck (Darmstadt, Germany). Phosphate Buffered Saline (PBS) Mg-/Ca- (Gibco®, Thermofisher, Carlsbad, CA, USA) was used for all experiments. AI334 Anti-Spike protein antibody was purchased from the antibody facility of the University of Geneva (Geneva, Switzerland). FITC-labeled secondary rabbit anti-mouse antibody was obtained from Dako (Glostrup, Denmark).
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2

DOTAP-DOPE Lipid Nanoparticle Preparation

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For all experiments, the lipids 1,2-dioleoyloxy-3-trimethylammoniumpropane (DOTAP) chloride (Mw: 698.54 g/mol) and 1.2 dioleoyl-sn,glycero-3-phosphoethanolamine (DOPE) were obtained from Sigma-Aldrich (Buchs, Switzerland), whereas dipalmitoylphosphatidylcholine (DPPC) was purchased from Avanti Polar Lipids (Alabaster, AL, USA). Methanol, ethanol and isopropanol were acquired from Fisher Scientific (Leicester, UK) and Chloroform Emprove® from Merck (Darmstadt, Germany). Phosphate buffer saline (PBS) Mg-/Ca- (Gibco®, Thermo Fisher, Grand Island, NY, USA) was used for all experiments. The AI334 Anti-Spike protein antibody was purchased from the antibody facility of the University of Geneva (Geneva, Switzerland). An FITC-labeled secondary rabbit anti-mouse antibody was obtained from Dako (Glostrup, Denmark).
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