siRNA targeting human Tks5, control siRNA, GST, and Myc (clone 9E10) antibodies, goat anti-Tks5 polyclonal antibody (M-20), and p21 antibody were from Santa Cruz Biotechnology (Santa Cruz, CA). Rabbit anti-Tks5 polyclonal antibody was a gift of S. Courtneidge (Sanford/Burnham Medical Research Institute, La Jolla, CA). XB130 monoclonal antibody was generated as previously described (Xu et al., 2007 (link)). XB130 siRNA and control siRNA were from GE Healthcare Dharmacon (Lafayette, CO). RGS-His antibody to detect 6xHis-tagged proteins was from Qiagen (Valencia, CA). Src (clone GD11) monoclonal antibody, phosphotyrosine (clone 4G10) monoclonal antibody, and GADPH antibody were from Upstate Biotechnology (Lake Placid, NY). Horseradish peroxidase–conjugated goat anti-mouse or anti-rabbit secondary antibodies were from Amersham Pharmacia Biotech (Piscataway, NJ). PI3K p85α, phosphotyrosine 458 PI3K, Akt, and phosphoserine 473 Akt antibodies were from Cell Signaling Technology (Beverly, MA). Anti-GAPDH antibodies were from Abcam (Cambridge, United Kingdom). Human Ki67 antibody and rabbit anti-Tks5 (SH3#1) were from EMD Millipore (Merck, Darmstadt, Germany).
Horseradish peroxidase conjugated goat anti mouse or anti rabbit secondary antibodies
Manufactured by Cytiva
Horseradish peroxidase–conjugated goat anti-mouse or anti-rabbit secondary antibodies are laboratory reagents used in immunoassays and immunochemical techniques. These antibodies are designed to bind to primary antibodies raised in mouse or rabbit, and the conjugated horseradish peroxidase enzyme can be used to detect and quantify the targeted protein or antigen.
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Lab products found in correlation
Control sirna, by Santa Cruz Biotechnology (1 mentions)
Anti gapdh antibody, by Abcam (1 mentions)
Pi3k p85α, by Cell Signaling Technology (1 mentions)
Phosphoserine 473 akt, by Cell Signaling Technology (1 mentions)
Rabbit anti tks5 sh3 1, by Merck Group (1 mentions)
P21 antibody, by Santa Cruz Biotechnology (1 mentions)
Ecl reagent, by Cytiva (1 mentions)
Anti actin, by Merck Group (1 mentions)
2 protocols using horseradish peroxidase conjugated goat anti mouse or anti rabbit secondary antibodies
1
Tks5 Knockdown and Protein Detection
2
Histone Acetylation Profiling in Colon Cancer
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Whole cell extracts prepared from HCT116, SW480 and TC7 colon cancer cell lines were separated on a 12% SDS-PAGE gel and subjected to immunoblot analysis. Primary antibody for Western Blot analysis was AcH3 (5 μg/ml Millipore) and anti-actin (1/15000, Millipore®). Protein expression was detected by using horseradish peroxidase-conjugated goat anti-mouse or anti-rabbit secondary antibodies (Amersham) with ECL reagent (Amersham) following the manufacturer's instructions.
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