At 10 weeks of age, control mice and mice raised on HFD were provided with Bio Serv Mouse Igloos® equipped with either Fast-Trac accessory running discs or nothing. After four weeks of access to the Igloos with or without running discs, mice underwent the food preference test described previously (section 2.3 ). In order to quantify the amount of exercise achieved by control and HFD-raised animals, a separate cohort of animals raised on either diet were individually housed with ad libitum access to drinking water and food (control diet or HFD). Each cage was also provided with a Bio Serv Mouse Igloo® equipped with a Fast-Trac accessory running disc. A magnetic sensor was attached to the igloo and two permeant magnets were attached to the running discs to allow the recording and analysis of disc turns through the ClockLab collection and analysis system (Actimetrics, Wilmette, IL). Seven days of recoding was collected for each animal and data from days 4 and 5 were averaged for the final comparison.
Mouse igloo
Manufactured by Bio-Serv
Sourced in Italy, United States
The Mouse Igloo is a compact and sheltered housing structure designed for laboratory mice. It provides a safe and enclosed environment for the mice to live in during experiments or housing. The igloo is made of durable, chew-resistant materials and can be easily cleaned and maintained.
Automatically generated - may contain errors
7 protocols using mouse igloo
1
Measuring Exercise Behavior in Mice
2
R6/2 HD Mouse Maintenance and Characterization
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R6/2 HD mice were maintained by backcrossing HD males with B6/CBA F1 females. Tails were cut at approximately 3 weeks of age for genotyping. Mice were weaned at 3.5 weeks. Animals were maintained in a 12-h alternating dark-light cycle. Access to food and water was ad libitum. Each cage had a single mouse igloo® (Bio-Serv) and Sani Chip bedding (Harlan). CAG repeat sizes were monitored. Mice used in the neonatal iron supplementation study had a CAG repeat size in the range 180–185. Mice used in the adult iron supplementation study had a CAG repeat size in the range 150–160. The neonatal study was conducted first; due to expanding CAG repeat size in the colony, the adult study was conducted with a newly established colony. Based on previous studies in R6/2 mice, the target group sizes for behavioral studies were 10–16 animals, and for biochemistry and neuropathology studies 8–10 animals.
3
Genotyping and Housing of YAC128 HD Mice
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YAC128 HD mice were maintained on the FVB-inbred background. Tails snips were obtained at 3 weeks of age and mice were genotyped by PCR as previously described [21 (link)]. Female mice only were used for experiments. Mice were maintained on a 12-hour light-dark cycle and were provided with access to food and water ad libitum. Unless otherwise state mice were fed cereal-based AIN76A rodent chow that contains 150 ppm iron. Each cage had a mouse igloo (Bio-Serv) and sani-chip bedding (Harlan Laboratories). All procedures were approved by the Institutional Animal Care and Use Committee and followed NIH guidelines.
4
Characterization of YAC128 and APP Mice
5
Mouse Hoarding Behavior Assessment
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On the day of the assessment mice were individually placed in polysulfone cages 36.5 cm × 20.7 cm × 14 cm (Techniplast, Buguggiate, Italy) with a mouse igloo (BioServ, The Netherlands) and corncob bedding. Each cage was attached to a wire mesh tube (60 cm), at the far end of which were placed 100 g of normal diet food pellets (each weighing around 2 g) for the first dark phase, and 180 g of food pellets for the second dark phase. Water and food (3 g) was provided inside the cages. In the morning of the first test day mice were placed in the cages with the hoarding tube entrances blocked, in order to habituate. The tubes were opened by late afternoon, just before the start of the dark phase (Deacon, 2006a (link)). The next morning the pellets inside the cage, as well as those remaining in each tube, were weighed and counted. The hoarding test was repeated on the following day. Data were analyzed with non-parametric statistical test (Mann–Whitney) as the distribution of hoarding performance values deviated from normality according to the Shapiro–Wilk test.
6
Genotyping and Maintenance of YAC128 HD Mice
7
Subcutaneous Tumor Implantation in NRG Mice
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For the in vivo experiments, 6–10-week-old male NRG mice were purchased from the BC Cancer Research Institute Animal Resource Centre (ARC, Vancouver, BC, Canada). Mice were caged in autoclaved Allentown ventilated caging at a capacity of 2–4 animals/cage for the length of the study. Cages were changed biweekly and included Nestlets (Ancare, Bellmore, NY, USA) as environmental enrichment, transparent tinted polycarbonate Mouse Igloos (Bio-Serv, Flemington, NJ, USA), and Envigo 7097 ¼” corn cob bedding. All enrichment was added to the cages prior to the cages being autoclaved. Mice were fed Envigo Teklad Global Rodent Diet 2920 (Indianapolis, IN, USA). The rodent food was kept in the hoppers of the wire lids and was changed biweekly. Reverse-osmosis water was supplied through Avidity Science automatic watering valves at a flow rate of 25–50 mL/min. The environmental control of the lights and the monitoring of temperature, humidity, and airflow was performed by WatchDogEX™ (Waterford, WI, USA). On the first day of the experiment, 5 × 106 tumour cells were implanted subcutaneously into each mouse’s back. The volumes of the tumours were determined using the following formula: L × W2 × 0.5.
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