Equal amounts of protein from gastric mucosal lysates were subjected to SDS-PAGE analysis, and immunoblotting using the appropriate antibodies was performed as we previously described.15 (link) The incubation condition for each antibody is as follows: anti-cPLA2 antibody (1:1,000; Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-COX-1 antibody (1:1,000; Santa Cruz Biotechnology), anti-COX-2 antibody (1:1,000, Cayman Chemical), anti-HO-1 antibody (1:1,000; Abcam Inc., Cambridge, UK), anti-NQO-1 antibody (1:1,000; Abcam Inc.), anti-GCLC (1:1,000; Abcam Inc.), or anti-GCLM (1:1,000; Abcam Inc.).
Anti cpla2 antibody
Manufactured by Santa Cruz Biotechnology
Sourced in United States
The Anti-cPLA2 antibody is a research-use-only product that specifically targets the cytosolic phospholipase A2 (cPLA2) protein. cPLA2 is an enzyme involved in the release of arachidonic acid, which is a precursor to various inflammatory mediators. This antibody can be used to detect and study the expression and localization of cPLA2 in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Anti cox 1 antibody, by Santa Cruz Biotechnology (1 mentions)
Anti ho 1 antibody, by Abcam (1 mentions)
Anti nqo1 antibody, by Abcam (1 mentions)
Anti gclc, by Abcam (1 mentions)
Anti cox 2 antibody, by Cayman Chemical (1 mentions)
Anti gclm, by Abcam (1 mentions)
Anti beclin 1, by Cell Signaling Technology (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions)
Phalloidin tetramethylrhodamine b isothiocyanate, by Merck Group (1 mentions)
Anti β actin antibody, by Merck Group (1 mentions)
Complete protease inhibitor cocktail, by Roche (1 mentions)
Anti cox 2, by Cell Signaling Technology (1 mentions)
2 protocols using anti cpla2 antibody
1
Gastric Mucosal Protein Expression Analysis
2
Detailed Lipid Analysis Protocol
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All commercial chemicals were of the highest available grade. Solvent solutions were from Carlo Erba (Milano, Italy). All powdered reactants, solutions for electrophoresis, Phalloidin Tetramethylrhodamine B isothiocyanate, DAPI, and anti-β-actin antibody, were from Sigma Chemical Co (St. Louis, MO, USA). Lipid standards, FA s, neutral lipids, PL, and Avanti Polar Lipids were from Sigma Chemical Co. (St. Louis, MO, USA). The 5% CO2:95% N2 gas cylinder came from Sapio (Monza, Italy). Collagen I rat tail solution for RBE4 cell culture, BODIPY 493/503, and Alexa Fluor® 567-labeled goat anti-rabbit IgG were from Invitrogen, Life Technologies Italia Fil. (Monza, MB, Italy). Stock solutions for RBE4 cell culture included: alpha-MEM medium with glutamax-1 and Ham’s F-10 nutrient medium, geneticin solution antibiotic, and Euroclone (Milano, MI, Italy). The complete protease inhibitor cocktail came from Roche Diagnostics S.p.A (Milano, Italy). Anti-cPLA2 antibody came from Santa Cruz Biotechnology Inc. (Santa Cruz, CA, USA). Anti-Cox-2, anti-LC3-I/II anti-p62, and anti-Beclin-1 antibodies were from Cell Signaling Technology (Danvers, MA, USA). Secondary horseradish peroxidase (HRP) -conjugated antibodies and enhanced chemiluminescence (ECL) SuperSignal detection kit were from Pierce (Rockford, IL, USA).
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