Samples were taken after aggregation for 72 hours in the 384-well plates and collected by same conditions criteria. They were prepared for electron microscopy by using the conventional negative staining procedure. An aliquot (20 μL) of solution was absorbed on Formvar-carbon-coated grids for 2 min, blotted, and negatively stained with uranyl acetate (1%) for 1 min. Grids were examined with a TEM (Jeol JEM-1400, JEOL Inc, Pea- body, MA, USA) at 80 kV. Images were acquired by using a digital camera (Gatan Orius, Gatan Inc, Pleasanton, CA, USA) at 4000 or 25000 magnifications.
Gatan orius
Manufactured by Ametek
Sourced in United States
The Gatan Orius is a high-performance digital camera designed for electron microscopy applications. It offers high-resolution imaging capabilities, fast data acquisition, and versatile software integration. The core function of the Gatan Orius is to capture and record high-quality digital images from electron microscopes.
Automatically generated - may contain errors
4 protocols using gatan orius
1
Negative Staining Electron Microscopy
2
Negative Staining of Extracellular Vesicles for TEM
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
EVs were isolated as described previously and resuspended in 50 µL Tris base buffer (100 mM, pH 7.4). EV samples were prepared for TEM using the conventional negative staining procedure. Briefly, 10 µL EV samples were absorbed for 2 min on formvar-carbon-coated copper grids preliminarily ionized using the PELCO easiGlow™ Glow Discharge Cleaning System (Ted Pella Inc., Redding, California, United States). Preparations were then blotted and negatively stained with 1% uranyl acetate for 1 min. Grids were examined using an 80 kV JEM-1400 electron microscope (JEOL Inc., Peabody, Massachusetts, United States) and images acquired with a digital camera (Gatan Orius, Gatan Inc., Pleasanton, California, United States).
3
Ultrastructural Analysis of Mouse Skin
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mouse skin was fixed with 2.5% glutaraldehyde and 2% paraformaldehyde in 0.1 M cacodylate buffer, pH 7.2 (EMS), for 24 h at 4°C and post-fixed at 4°C with 1% OsO4 and 1.5% K3Fe(CN)6 in the same buffer. The Samples were treated for 1 h with 1% aqueous uranyl acetate, dehydrated in a graded series of acetone, and embedded in EMbed 812 resin (EMS). After 48 h of polymerization at 60°C, the ultrathin sections (80 nm) were mounted on 75 mesh Formvar carbon–coated copper grids. The sections were stained with UranyLess (Delta Microscopies) and lead citrate. Grids were examined with a TEM (Jeol JEM-1400; JEOL Inc) at 80 kV. Images were acquired using a digital camera (Gatan Orius; Gatan Inc).
4
Negative Staining TEM Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Solutions were collected from the fluorescence microplate after 120 to 150 h and prepared for TEM using the conventional negative staining procedure. An amount of 20 μL of the solution was adsorbed on Formvar-carbon-coated grids for 2 min, blotted and negatively stained with uranyl acetate (1%) for 1 min. Grids were examined with a TEM (Jeol JEM-1400, JEOL Inc, Peabody, MA, USA) at 80 kV. Images were acquired using a digital camera (Gatan Orius, Gatan Inc, Pleasanton, CA, USA) at 10,000× or 25,000× magnification. Since the phosphate buffer reacts with uranyl acetate, HEPES buffer was preferred and employed for TEM experiments, and thus, for ThT fluorescence experiments as well.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!