Sulphuric acid (95–98%), nitric acid (69%), ethanol (99%) and chloroform were purchased from PANREAC, S.A.U. (Barcelona, Spain). N,N′-Diisopropylcarbodiimide (DIC, 98%), N-hydroxysuccinimide (NHS, 97%), triethylamine (TEA, 99%), cysteamine hydrochloride (98%), N-Boc-ethylenediamine (Boc-EDA, 98%), cellulose microcrystalline (50 μm particle size), acetone (99.5%), 1-butyl-3-methylimidazolium tetrafluoroborate (BMIM-BF4, 97%), dimethyl sulfoxide (DMSO, 99.9%), sodium carbonate (99.95%). 5-Aminosalicylic acid and the diisocyanates purchased from Sigma–Aldrich, while the multi walled carbon nanotubes were purchased from Bayer (Germany). NMR spectra were measured on a Bruker Avance-400.
Chloroform
Manufactured by ITW Reagents
Sourced in Spain, Saudi Arabia
Chloroform is a colorless, volatile liquid chemical compound commonly used in laboratory settings. It has the chemical formula CHCl3 and is known for its high density and low flammability. Chloroform is primarily used as a solvent for various organic compounds and in the synthesis of other chemicals.
Automatically generated - may contain errors
Lab products found in correlation
Methanol, by ITW Reagents (10 mentions)
Hydrochloric acid (hcl), by ITW Reagents (7 mentions)
Hexane, by ITW Reagents (5 mentions)
Acetone, by ITW Reagents (5 mentions)
Ethyl acetate, by ITW Reagents (4 mentions)
Ethanol, by ITW Reagents (3 mentions)
Potassium chloride (kcl), by ITW Reagents (3 mentions)
Potassium hydroxide, by ITW Reagents (3 mentions)
2 propanol, by ITW Reagents (3 mentions)
19 hydroxycholesterol, by Steraloids (3 mentions)
5α cholestane, by Merck Group (3 mentions)
Sulphuric acid, by ITW Reagents (2 mentions)
Hplc grade methanol, by ITW Reagents (2 mentions)
Toluene, by ITW Reagents (2 mentions)
Aliquat 336, by Merck Group (2 mentions)
Sodium thiosulfate, by ITW Reagents (2 mentions)
Petroleum ether, by ITW Reagents (2 mentions)
Isooctane, by ITW Reagents (2 mentions)
Acetonitrile, by ITW Reagents (2 mentions)
Sodium hydroxide (naoh), by ITW Reagents (2 mentions)
26 protocols using chloroform
1
Functionalization of Cellulose Microcrystalline
2
HPLC-grade Solvent Preparation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Chloroform and methanol HPLC grade were purchased from Panreac (Spain). D2O 99.9% was purchased from Armar Chemicals (Switzerland). All other chemicals were purchased from Sigma-Aldrich (USA). H2O was deionized using an Ultra Clear UV plus TM water system (SG water, Germany) to the quality of 18.2 MOhm.
3
Polymer Scaffold for Cell Culture
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Ethylene brassylate (EB) monomer was supplied by Sigma Aldrich (Spain), while D,L-lactide and L-lactide monomers were provided by Corbion (The Netherlands). The triphenyl bismuth (Ph3Bi) catalyst was obtained from Gelest (USA). Dichloromethane and n-Hexane were supplied by Labbox (Spain). Multi-Walled Carbon Nanotubes (MWCNT), having an average diameter of 10–15 nm with 5–15 walls and 1–10 μm in length, were supplied by Arkema (France). Dulbecco’s Modified Eagle Medium (DMEM), Hanks’ Balanced Salt Solution (HBSS), Penicillin Streptomycin (P/S), Fetal Bovine Serum (FBS), AlamarBlue cell viability reagent and rhodamine-phalloidin were supplied by Fisher Scientific (Spain). Laminin, PBS (Phosphate-buffered saline), Diiodomethane, Fluoroshield with DAPI, Triton X-100 and Tween 20 were supplied by Sigma Aldrich (Spain), and 16% Formaldehyde solution was supplied by Thermo Fisher Scientific (USA). Chloroform was supplied by PanReac (Spain).
4
Synthesis of Monodisperse Nanoparticles
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
In this study was used: iron (III) acetylacetonate (99%) from Sigma Aldrich (St. Louis, MO, USA), oleic acid (90%) from Panreac (Barcelona, Spain), benzyl ether (99%) from Sigma Aldrich (Madrid, Spain), 4-biphenylcarboxylic acid (99%) from Sigma Aldrich (Madrid, Spain), toluene (99.8%) from Panreac (Barcelona, Spain), hexane (99%) from Panreac (Barcelona, Spain), chloroform (99.9%) from Panreac (Barcelona, Spain), and nitrogen (99%) from Air Liquide (Paris, France). All solutions were made with distilled water.
5
Preparation of Highly Pure Reagents
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Chloroform and methanol were purchased from PanReac (Spain). D2O 99.9% was purchased from Armar Chemicals (Switzerland). All other chemicals were purchased from Sigma-Aldrich (USA). H2O was deionized using Ultra Clear UV plus water system (SG Water, Germany) to the quality of 18.2 MΩ.
6
Quantification of Sulfonamide Residues
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Ammonia solution (30%), acetic acid (99.5%), n-hexane (95%), ammonium acetate (98%), were purchased from Carlo Erba Reagents (Rodano, Italy). Chloroform and acetone (analytical grade) were obtained from Panreac (Barcelona, Spain). Water, acetonitrile and methanol of HPLC grade were purchased from Baker (Deventer, Holland). The 0.02 M acetate buffer solution, used as solution (A) for HPLC mobile phase, was prepared by dissolving 1.54 g of ammonium acetate in water. The pH value was adjusted to 4.50±0.02 with acetic acid. Standards of SAs [sulfadiazine (SDA) 99.3%, sulfathiazole (STZ) 99.9%, sulfapyridine (SP) 99.7%, sulfamerazine (SM) 98.8%, sulfamethazine (SMZ) 99.8%, sulfamethoxipyridazine (SMP) 99.7%, sulfachloropyridazine (SCP) 99.4%, sulfamonomethoxine (SMM) 98%, sulfamethoxazole (SMX) 99.9%, sulfadoxine (SDO) 99.8%, sulfaphenazole (SNZ) 99%, sulfadimethoxine (SDM) 99.8% and sulfaquinoxaline (SQX) 96%] were supplied by Sigma-Aldrich (Steinheim, Germany).
Working standards at concentration of 0.25-0.5-1.0-2.0-4.0 mg/L were prepared by appropriate dilution in mobile phase to make calibrations curves for the detection of SAs in meat. A feed sample fortified at five concentrations (0.2, 1.0, 5.0, 10.0 and 20.0 mg/kg) with standard solutions of SAs before the extraction was used to obtain the calibration curve for the analysis of SAs in feed.
Working standards at concentration of 0.25-0.5-1.0-2.0-4.0 mg/L were prepared by appropriate dilution in mobile phase to make calibrations curves for the detection of SAs in meat. A feed sample fortified at five concentrations (0.2, 1.0, 5.0, 10.0 and 20.0 mg/kg) with standard solutions of SAs before the extraction was used to obtain the calibration curve for the analysis of SAs in feed.
7
Polymer-Supported Aliquat 336 Sorbents for Arsenate Removal
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Aliquat 336 was obtained from Sigma-Aldrich (USA), whereas the polymers CTA and PVC were purchased from Fluka (Bern, Switzerland). Chloroform (for CTA) and tetrahydrofuran, THF (for PVC), both from Panreac (Barcelona, Spain), were used to dissolve the polymer and the ILs. NaSCN and NaNO3 (Panreac, Barcelona, Spain) were used as the anion source to prepare the Aliquat 336 derivatives.
Stock solution (100 mg L−1) of As(V) was prepared from solid Na2HAsO4·7H2O purchased from Merck (Darmstadt, Germany). Working solutions of arsenate in ultrapure water were prepared by dilution of the corresponding stock solution. Sodium chloride, obtained from Fluka (Bern, Switzerland), was used to prepare the stripping solution.
All reagents and solvents were of analytical reagent grade. Ultrapure water obtained from a Milli-Q Plus water purification system (Millipore Ibérica, Madrid, Spain) was used.
Stock solution (100 mg L−1) of As(V) was prepared from solid Na2HAsO4·7H2O purchased from Merck (Darmstadt, Germany). Working solutions of arsenate in ultrapure water were prepared by dilution of the corresponding stock solution. Sodium chloride, obtained from Fluka (Bern, Switzerland), was used to prepare the stripping solution.
All reagents and solvents were of analytical reagent grade. Ultrapure water obtained from a Milli-Q Plus water purification system (Millipore Ibérica, Madrid, Spain) was used.
8
Simulated Natural Water Preparation and PIM Fabrication
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The reagents used for the preparation of the simulated natural water (SNW) were sodium sulfate (Merck, Madrid, Spain), calcium chloride hexahydrate (Panreac, Barcelona, Spain), and sodium hydrogen carbonate (Merck, Madrid, Spain). The concentration of the salts in SNW was: 2 × 10−3 M NaHCO3, 0.75 × 10−3 M CaCl2, and 0.25 × 10−3 M Na2SO4, and the measured pH was 8.3.
For the preparation of PIMs, the following reagents were used: cellulose triacetate (Acros Organics, Fisher Scientific, Madrid, Spain), and the extractant trioctylmethylammonium chloride (Aliquat 336®) (Sigma-Aldrich, Steinheim, Germany). Chloroform (Panreac, Bercelona, Spain) was used as a solvent and ultrapure water with resistivity ≥18 μS cm−1 was taken from a MilliQ system (Millipore Ibérica S.A., Barcelona, Spain).
Standard solutions of 1000 mg L−1 of As and P (Sigma-Aldrich, Steinheim, Germany) were used for the measurement of the elements in the aqueous solutions.
All the reagents were of analytical grade purity.
For the preparation of PIMs, the following reagents were used: cellulose triacetate (Acros Organics, Fisher Scientific, Madrid, Spain), and the extractant trioctylmethylammonium chloride (Aliquat 336®) (Sigma-Aldrich, Steinheim, Germany). Chloroform (Panreac, Bercelona, Spain) was used as a solvent and ultrapure water with resistivity ≥18 μS cm−1 was taken from a MilliQ system (Millipore Ibérica S.A., Barcelona, Spain).
Standard solutions of 1000 mg L−1 of As and P (Sigma-Aldrich, Steinheim, Germany) were used for the measurement of the elements in the aqueous solutions.
All the reagents were of analytical grade purity.
9
Antioxidant Extraction from Guara Almond Skins
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Water (ultrapure grade) and ethanol (HPLC grade) were acquired from Merck (Madrid, Spain). Hexanal (98%), p-anisidine reagent (99%), 2,2-diphenyl-1-picrylhydrazyl (DPPH), sodium chloride, methanol (HPLC grade) and n-hexane (96%, GC grade) were supplied by Sigma-Aldrich (Madrid, Spain). Petroleum ether, sodium methylate, sulphuric acid (98%), acetic acid, potassium iodide, sodium thiosulfate (0.1 M), chloroform, isooctane were of analytical or chromatographic grade and were purchased from Panreac (Barcelona, Spain). Standard compounds such as linoleic (C18:2), oleic (C18:1), palmitic (C16:0), palmitoleic (C16:1), stearic (C18:0) and tridecanoic (C13) acid methyl esters; and 4-methyl-2-pentanone were acquired from Sigma-Aldrich (Madrid, Spain) in the purest available form. A commercial poly(ε-caprolactone) PCL-CAPA 6800 (Mn = 80,000, density = 1.1 g cm−3) was supplied in pellets by Perstorp Holding AB (Sweden). Guara almond skins (AS) were kindly supplied by Almendras Llopis (Alicante, Spain).
10
Analytical Reagent Procurement Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Chloroform, methanol, and acetonitrile (HPLC grade) were purchased from Panreac (Barcelona, Spain). D2O 99.9% was purchased from Armar Chemicals (Dottingen, Switzerland). All other chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA). H2O was deionized using Ultra Clear UV plus TM water system (SG water, Hamburg, Germany) to the quality of 18.2 MOhm.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!