Anti-HA antibody (#3F10) was purchased from Roche Diagnostics; anti-CERT (#ab72536) from Abcam; chicken antibody against VAP (Kumagai et al., 2014 (link)) anti-TGOLN2/TGN46 (#A304-434A) from Bethyl Laboratories; anti-TOMM20 (#WH0009804M1) from Sigma-Aldrich; anti-Lamp2 (#sc-18822) from Santa Cruz; anti-LBPA (#z-PLBPA) from Echelon Bioscience; anti-EEA1 (#610457) from BD Transduction Laboratories; anti-catalase (#D4P7B) and anti-Rab11a (#2413) from Cell Signaling Technology; anti-Hrs (#10390-1-AP) from Proteintech; anti-GFP (#04404-84) from Nacalai Tesque, and anti-GAPDH (016-25523) from Fujifilm Wako Pure Chemical Corporation. 2-Bromohexadecanoic acid (2-BP, #M1177) was purchased from Sigma-Aldrich. Lipid dye II (#LD02) was from Dojindo Laboratories. Lysenin was a gift from Dr. Sekizawa (Zenyaku Kogyo). GFP-lysenin was a gift from Dr. Kobayashi (Riken). [14C(U)]L-serine (156 mCi/mmol, #MC265) was from Moravec.
Anti tomm20
Manufactured by Merck Group
Anti-TOMM20 is a laboratory reagent used for the detection and analysis of the TOMM20 protein, which is a component of the translocase of the outer mitochondrial membrane complex. This antibody can be used in various experimental techniques, such as Western blotting, immunohistochemistry, and immunofluorescence, to study the localization and expression of TOMM20 in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Anti eea1, by BD (1 mentions)
Anti lamp2 sc 18822, by Santa Cruz Biotechnology (1 mentions)
Anti catalase d4p7b, by Cell Signaling Technology (1 mentions)
Anti rab11a, by Cell Signaling Technology (1 mentions)
Anti gfp, by Nacalai Tesque (1 mentions)
Anti gapdh, by Fujifilm (1 mentions)
2 bromohexadecanoic acid 2 bp, by Merck Group (1 mentions)
Anti ha antibody, by Roche (1 mentions)
Alexa fluor 555, by Jackson ImmunoResearch (1 mentions)
Anti ha, by Roche (1 mentions)
Metamorph microscopy automation and image analysis software, by Molecular Devices (1 mentions)
2 protocols using anti tomm20
1
Comprehensive Antibody Inventory for Cell Organelle Labeling
2
Quantifying FECH Accumulation in Nucleoli After UV Exposure
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cells were fixed with 3.7% paraformaldehyde (PFA) for 12 min, permeabilized in PBS containing 0.1% Triton X-100, hybridized with anti-HA (Roche, 11867423001; 1:250), anti-FECH (S. Cruz, sc-377377; 1:40) or anti-TOMM 20 (Sigma) antibodies and afterwards with secondary antibodies conjugated to Alexa Fluor 448 or Alexa Fluor 555 (Jackson Immunoresearch). Nuclei were counterstained with DAPI. Images were acquired using an Olympus IX71 inverted microscope equipped with a CCD camera (Robert Scientific Photometrics) and analysed using the MetaMorph Microscopy Automation and Image Analysis Software (Molecular Devices). The quantification of FECH accumulation inside the nucleolus was performed on immunostained cells 1, 2, 3 or 4 h after UV-C irradiation (10 J/m2). For each cell strain, at least 200 nuclei were analysed. Exposure time, binning, microscope settings and light-source intensity were kept constant for all the samples.
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