Pcr based

K562 cells, acquired from the UC Berkeley Cell Culture Facility, were maintained in IMDM supplemented with 10% fetal bovine serum (FBS), 1% sodium pyruvate, 1% non-essential amino acids and 100ug/ml penicillin-streptomycin. Cells were tested for mycoplasma contamination using enzymatic (Lonza, Basel, Switzerland) and PCR-based assays (Bulldog Bio).

HUDEP-2 cells were acquired from the Nakamura Lab at the RIKEN BioResource Center in Japan and were cultured in StemSpan SFEM (Stem Cell Technologies) supplemented with 50ng/ml SCF (R&D Systems), 3IU/ml EPO (KIRIN or Peprotech), 10^-6M Dexamethasone (Dex, SIGMA-D2915), and 1ug/ml Doxycycline (Dox) (TAKARA Bio), and 100ug/ml penicillin-streptomycin, unless otherwise noted. Note: HUDEP-2 cells can proliferate in the absence of Dex for a short period, however we cultured the cells in Dex-containing medium for a long-term culture. SCF, EPO, and Dox are essential for culturing HUDEP-2 cells. Cells were split 1:6 or 1:10 every 3 or 4 days and density was maintained below 10⁶ cells/ml as high cell density deteriorated cell viability. Cells were tested for mycoplasma contamination using enzymatic (Lonza) and PCR-based assays (Bulldog Bio).