The human pancreatic cancer cell lines MIA PaCa-2, AsPC-1, PANC-1 and BxPC-3 were purchased from American Type Culture Collection (CRL-1420TM, CRL-1682, CRL-1469 and CRL-1687, respectively, ATCC; Rockville, MD, USA) and banked at Centre Paul Strauss. Cells were cultured at 37 °C in a humidified atmosphere containing 5% CO2 and 95% air. PANC-1 was cultured in Dulbecco’s modified Eagle’s medium (DMEM; PAN Biotech GmbH, Aidenbach, Germany) supplemented with 10% fetal bovine serum (FBS, PAN Biotech GmbH) and 1% of a solution of penicillin (10000 IU/mL) and streptomycin (10 mg/mL) (PAN Biotech GmbH). MIA PaCa-2 was cultured in the same conditions with 1% of 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES, 1 mM, PAN Biotech GmbH), 1% of sodium pyruvate (10 mM, PAN Biotech GmbH) and 1% of non-essential amino acids (NEAA, PAN Biotech GmbH). AsPC-1 and BxPC-3 were cultured in Roswell Park Memorial Institute medium (RPMI; PAN Biotech GmbH) supplemented with 10% FBS, and 1% of a solution of penicillin (10000 IU/mL) and streptomycin (10 mg/mL). Subconfluent cell monolayers were trypsinized once a week using 0.5% trypsin containing 2% EDTA (PAN Biotech GmbH) and plated at passage ratios between 0.25:10 to 1:10, according to the cell line or used directly for study after enumeration determined with a Countess® Cell Counter (Countess, Invitrogen, Carlsbad, CA, USA).
Crl 1682
Manufactured by American Type Culture Collection
Sourced in France
The CRL-1682 is a cell line of human endothelial cells derived from the umbilical vein. It is maintained in culture and can be used for various research applications.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin streptomycin, by Thermo Fisher Scientific (3 mentions)
Aspc 1, by American Type Culture Collection (2 mentions)
Tib 202, by American Type Culture Collection (2 mentions)
Mia paca 2, by American Type Culture Collection (1 mentions)
Bxpc 3, by American Type Culture Collection (1 mentions)
Panc 1, by American Type Culture Collection (1 mentions)
Countess cell counter, by Thermo Fisher Scientific (1 mentions)
Crl 1469, by American Type Culture Collection (1 mentions)
Crl 1687, by American Type Culture Collection (1 mentions)
Fetal bovine serum (fbs), by PAN Biotech (1 mentions)
Non essential amino acids (neaa), by PAN Biotech (1 mentions)
Sodium pyruvate, by PAN Biotech (1 mentions)
Panc 1, by PAN Biotech (1 mentions)
Mia paca 2, by PAN Biotech (1 mentions)
4 2 hydroxyethyl 1 piperazineethanesulfonic acid hepes, by PAN Biotech (1 mentions)
Bxpc 3, by PAN Biotech (1 mentions)
Penicillin, by PAN Biotech (1 mentions)
Streptomycin, by PAN Biotech (1 mentions)
Hct116, by American Type Culture Collection (1 mentions)
T75 flask, by BD (1 mentions)
5 protocols using crl 1682
1
Culturing Pancreatic Cancer Cell Lines
2
Culturing Cancer Cell Lines
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CT-26 (murine colon cancer), HCT-116 (human colon cancer), CRL-1739 (human stomach gastric cancer) and CRL-1682 (human pancreatic cancer) cell lines were purchased form ATCC. All cells were cultured in T-75 flasks (Falcon). All media were purchased from Life Technologies and supplemented with 10% fetal bovine serum (Sigma) and 1% penicillin-streptomycin (Fisher Scientific). CT-26 and CRL-1682 were cultured in RPMI-1640 medium. CRL-1739 and HCT-116 were cultured in DMEM medium. All cells were cultured in a 37° C, 5% CO2 incubator.
3
Culturing Human Pancreatic and Leukemia Cells
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Pancreatic human cell line was purchased from ATCC (LGC Standards, Molsheim, France). Cells were kept at 37 °C in humidified atmosphere containing 5 % (v/v) CO2 during their exponential growing phase and during incubation with investigated compounds. Confluency adherent cells were trypsinized and sub-cultured twice a week. Human pancreatic (AsPC-1, ATCC® CRL-1682) cell lines were maintained in DMEM high glucose medium (Dominique Dutscher, 67172 Brumath, France, Cat No L0102-500), while human acute monocytic leukemia cell line (THP-1, ATCC® TIB-202) was maintained in RPMI-1640 medium (ATCC® 30-2001™, LGC Standards S.a.r.l., Molsheim, France), supplemented with 10% (v/v) heat-inactivated fetal bovine serum (FBS, Life Technologies, Paisley, UK, Cat No 10270-106) and 1% (v/v) penicillin–streptomycin (105 units/mL and 10 mg/mL, Life Technologies, Paisley, UK, Cat No 15140-122).
4
Cell Line Maintenance Protocol
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All human cell lines purchased from ATCC (LGC Standards, Molsheim, France) Human mammary (MCF-7, ATCC® HTB-22), pancreatic (AsPC-1, ATCC® CRL-1682) and colon (SW-620, ATCC® CCL-227™) adenocarcinoma cell lines were maintained in DMEM high-glucose medium (Dominique Dutscher, 67,172 Brumath, France, Cat No L0102-500), while the human acute monocytic leukemia cell line (THP-1, ATCC® TIB-202) was maintained in RPMI-1640 medium (ATCC® 30-2001™, LGC Standards, Molsheim, France), supplemented with 10% (v/v) heat-inactivated fetal bovine serum (FBS, Life Technologies, Paisley, UK, Cat No 10270-106) and 1% (v/v) penicillin-streptomycin (10,000 units/mL and 10,000 µg/mL, Life Technologies, Paisley, UK, Cat No 15140-122). Cells were kept at 37 °C in a humidified atmosphere containing 5% (v/v) CO2 during their exponential growing phase and in the course of incubation with investigated compounds. Before confluence, adherent cells were trypsinized and sub-cultured twice a week.
Investigated extracts were initially dissolved in dimethyl sulfoxide (DMSO) in a concentrated stock solution. Further dilutions to the experimental concentrations applied on the cells have been done in RPMI-1640 or DMEM media prior to each experiment, thus the final concentration of DMSO on treated cells was 0.5% (v/v) for the most applied concentrations.
Investigated extracts were initially dissolved in dimethyl sulfoxide (DMSO) in a concentrated stock solution. Further dilutions to the experimental concentrations applied on the cells have been done in RPMI-1640 or DMEM media prior to each experiment, thus the final concentration of DMSO on treated cells was 0.5% (v/v) for the most applied concentrations.
5
Cell Lines for Cancer Research
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Human carcinoma cells of breast [MDA-MB-231 (ATCC® HTB-26™)], prostate [LNCaP clone FGC (ATCC® CRL-1740™)], and pancreas [AsPC-1 (ATCC® CRL-1682™)] origin were obtained from American Type Culture Collection (ATCC) and cultured in medium designated by the provider for propagation and maintenance. All cell lines were used at low passage number and proven free of Mycoplasma.
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