The TPHs content of each sample was determined by ultrasonic treatment of soil extracted in a 1:1 (v/v) mixture of hexane and acetone (extraction method EPA 3550b). Each 2 g sample of soil was first mixed with 1 g anhydrous Na2SO4 and then extracted at 20 °C in 15 mL of the solvent with the aid of an ultrasound device delivering 250 W (Branson M8800). The resulting suspension was centrifuged (10,000g, 5 min) to remove soil particles. The procedure was repeated and the two extracts combined. The solvent was evaporated using a concentrator (Eppendorf vacufuge plus), and the residual TPH amount was determined gravimetrically [19] (link).
M8800
Manufactured by Emerson
Sourced in United States
The M8800 is a versatile lab equipment designed for a range of applications. It features precise temperature control and advanced monitoring capabilities. The device's core function is to provide accurate and reliable measurements, but its intended use is not specified.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using m8800
1
Determination of Soil TPH Content
2
Sample Preparation and Extraction
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One tablet from each sample was crushed and homogenized using a pestle and mortar; capsules were opened and homogenized as well. Then, 30 mg of this powder mixture (Sartorius Analytic AC 210S, Goettingen, Germany) was brought to suspension in 10 mL of a mixture of ethanol/ water (50/50 v/v%) and sonicated (M8800, Branson, Danbury, USA) for 15 min. Afterwards, the samples were centrifuged (Heraeus Multifuge 3SR, Thermo Scientific, Waltham, USA) at 894 g during 10 min.
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