Biochanin a

Manufactured by Yuanye Bio-Technology

Sourced in China, United States

Biochanin A is a naturally occurring isoflavone compound extracted from various plant sources. It functions as a phytoestrogen, exhibiting structural and functional similarities to the human hormone estrogen.

Automatically generated - may contain errors

Lab products found in correlation

2 protocols using biochanin a

Comprehensive Analysis of Fructus Puerariae

Check if the same lab product or an alternative is used in the 5 most similar protocols

A total of 13 batches of FP were collected from Zhejiang (S1), Guangdong (S2 and S3), Guangxi (S4, S5 and S6), Hebei (S8), Hunan (S9, S10, S11 and S12) and Sichuan (S13) provinces of China. Besides, the control herbs (S7) were purchased from the National Institutes for Food and Drug Control (Beijing, China). Reference standards (purity >98%) including rutin, daidzin, daidzein, and quercetin were obtained from the National Institutes for Food and Drug Control (Beijing, China). Glycitin, genistin, tectoridin, glycitein, genistein, tectorigenin, and biochanin A with the purities over 98% were purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). Nicotiflorin and ononin with the purities over 98% were bought from Chengdu Alfa Biotechnology Co., Ltd. (Chengdu, China). Meanwhile, formononetin and puerarin were bought from Chengdu Must Bio-Technology Co., Ltd.(Chengdu, China). HPLC grade acetonitrile (ACN) and methanol (MeOH) were obtained from Fisher Scientific (Fair Lawn, NJ, USA). Formic acid of HPLC grade was provided by Kermel Chemical Reagent Co., Ltd. (Tianjin, China) and pure water was obtained from Wahaha (Hangzhou, China). All the other reagents were of analytical grade.

Han J., Xu K., Yan Q., Sui W., Zhang H., Wang S., Zhang Z., Wei Z, & Han F. (2021). Qualitative and quantitative evaluation of Flos Puerariae by using chemical fingerprint in combination with chemometrics method. Journal of Pharmaceutical Analysis, 12(3), 489-499.

+ Open protocol

+ Expand

UPLC-MS/MS Analysis of Isoflavonoids

Check if the same lab product or an alternative is used in the 5 most similar protocols

An Ultimate 3000 Ultra High Performance Liquid Chromatography (UPLC) system coupled with LTQ-Orbitrap velos pro mass spectrometer was used (Thermo-Fisher, Waltham, MA, USA), as well as an Acquity UPLC I-Class UPLC system coupled with Xevo TQ-S micro triple quadrupole mass spectrometer (Waters, Milford, MA, USA).
The reference compounds of formononetin, ononin, daidzein, daidzin, genistin, genistein, calycosin-7-O-β-d-glucoside, biochanin A, and chlorogenic acid (purity > 98% for all) were all purchased from Shanghai Yuanye Biotechnology Co., Ltd. (Shanghai, China). Acetonitrile and formic acid were purchased from Fisher Scientific, San Jose, CA, USA Company (mass spec-grade). Deionized water was prepared by Milli-Q ultrapure water preparation system (Millipore Co., Ltd., Billerica, MA, USA). All other chemicals were purchased from Beijing Chemical Works (Beijing, China, analytical grade).

Luo L., Gao W., Zhang Y., Liu C., Wang G., Wu H, & Gao W. (2020). Integrated Phytochemical Analysis Based on UPLC-MS and Network Pharmacology Approaches to Explore the Quality Control Markers for the Quality Assessment of Trifolium pratense L.. Molecules, 25(17), 3787.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!