Cy5 alkyne

Manufactured by Merck Group

Cy5-alkyne is a fluorescent dye molecule that contains a cyanine5 fluorophore and an alkyne functional group. The alkyne group allows for the dye to be conjugated to other biomolecules, such as proteins or nucleic acids, through click chemistry reactions. Cy5-alkyne can be used as a labeling reagent in various bioanalytical applications.

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Lab products found in correlation

4 protocols using cy5 alkyne

Glycan Modification and Detection

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GDP-N₃-Fucose, CMP-N₃-sialic acid, UDP-N₃-GalNAc, UDP-N₃-GlcNAc, Biotinylated Alkyne and 4’,6-diamidino-2-phenylindole (DAPI) were from R&D Systems/Bio-techne. Recombinant human B4GalT1Y285L, GALNT2, FUT7, GCNT1, B3GNT6, EXT1/2, ST6Gal1, C1GalT1 and HPSE, and recombinant B. thetaiotaomicron O-GlcNAcase (OGA), P. heparinus heparinase III (Hep III), P. multocida hyaluronan synthase (HAS) and S. agalactiae hyaluronan lyase were also from R&D System/Bio-techne. Streptavidin-Alexa Fuor 555 and streptavidin-Alexa Fluor 488 were from ThermoFisher Scientific. Cy5-Alkyne, UDP-Gal and UDP-GlcA and all other small chemicals were from Sigma Aldrich.

Wu Z.L., Person A.D., Anderson M., Burroughs B., Tatge T., Khatri K., Zou Y., Wang L., Geders T., Zaia J, & Sackstein R. (2017). Imaging specific cellular glycan structures using glycosyltransferases via click chemistry. Glycobiology, 28(2), 69-79.

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Glycosylation Analysis of Therapeutic Antibodies

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Recombinant FUT2, FUT3, FUT6, FUT8, FUT9, MGAT1, B3GalT2, B4GalT1, ST3Gal4, ST3Gal3, ST6Gal1, Flavobacterium meningosepticum PNGase F, Streptococcus pyogenes EndoS, Influenza viral H1N1 neuraminidase and GDP-Azido-Fucose were from Bio-Techne. Cantuzumab, an anti-Muc1 therapeutic antibody, was from Creative Biolabs. NIST monoclonal antibody reference material 8671 was from the National Institute of Standards and Technology. Alkyne Alexa Fluor® 488 and alkyne Alexa Fluor® 555 were from Thermo Fisher Scientific. IgG glycan G0 was from Agilent. Cy5-alkyne, RNase B, fetal bovine fetuin and asialofetuin and all other chemical reagents were from Sigma-Aldrich.

Wu Z.L., Whittaker M., Ertelt J.M., Person A.D, & Kalabokis V. (2020). Detecting substrate glycans of fucosyltransferases with fluorophore-conjugated fucose and methods for glycan electrophoresis. Glycobiology, 30(12), 970-980.

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Chemical Synthesis Reagents and Cell Culture Media

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Standard reagents, solvents monomers and other materials for chemical synthesis were purchased from Sigma-Aldrich or ThermoFisher. High glucose DMEM ( phenol red free, cat. # 31053-028) was acquired from Gibco (Life technologies). High glucose DMEM (cat. # D6546), Trypsin-EDTA (cat. # T3924), foetal bovine serum (FBS, cat. # F7524), penicillin-streptomycin antibiotic solution (10 000 U penicillin and 10 mg mL -1 streptomycin, cat. # P0781), L-glutamine (200 mm, cat. # G7513) and DMSO (cat. # D2438) were purchased from Sigma-Aldrich. Hoechst 33342 (cat. # H1399) and Presto Blue 10× solution (cat. # A13262 100 mL) were acquired from Invitrogen. Cy5 alkyne was purchased from Sigma-Aldrich.

Cavanagh R.J., Monteiro P.F., Moloney C., Travanut A., Mehradnia F., Taresco V., Rahman R., Martin S.G., Grabowska A.M., Ashford M.B, & Alexander C. (2024). Free drug and ROS-responsive nanoparticle delivery of synergistic doxorubicin and olaparib combinations to triple negative breast cancer models. Biomaterials science, 12(7).

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Recombinant Glycosylation Enzymes Protocol

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Recombinant CK2, B4GalT1, ST6Gal1, PGK1, HK1, HK2, AKT1, FUT8, HIF1α, SREBP1c, NOD2, G6PD, PFKFB3, PFKFB4, PGK1, and PKM were from R&D Systems, Bio-techne. Alkyne-Alexa Flu-or® 488, alkyne-Alexa Fluor® 555 were from Thermo Fisher Scientific. Cy5-alkyne, MG132, Flag-Beads and FLAG peptide were purchased from Sigma-Aldrich. Fluorophore-conjugated CMP-Sialic acids were prepared as previously described 1 .

Wu Z.L., Luo A., Grill A.E., Lao T., Zou Y., & Chen Y. (2020). Fluorescent Detection ofO-GlcNAc via Tandem Glycan Labeling.

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