The cells were harvested using serum-free media and seeded into the upper chambers of polycarbonate membrane filters with a pore size of 8.0 μm (Corning Incorporated, Corning, NY, USA) at a density of 1 × 105/mL. Subsequently, 600 μL of media containing 10% FBS was added to the lower chambers, and the cells were incubated at 37°C for 36 hours. To minimize potential bias in the results, three replicate wells were established for each experimental group, and each experiment was repeated thrice. The field of view for cell counting was randomly selected, and images were concurrently captured. Using Image J software, the number of migrating cells was quantified.
Polycarbonate membrane filters with a pore size of 8.0 μm
Manufactured by Corning
Sourced in United States
Polycarbonate membrane filters with a pore size of 8.0 μm. These filters are designed for filtration applications that require a consistent pore size and uniform flow characteristics.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using polycarbonate membrane filters with a pore size of 8.0 μm
1
Transwell Migration Assay for Cell Motility
2
Cell Migration Assay Protocol
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The cell lines used in this study were purchased from Procell (https://www.procell.com.cn/ ). The cells were harvested using serum-free media and seeded into the upper chambers of polycarbonate membrane filters with a pore size of 8.0μm (Corning Incorporated, Corning, NY, USA) at a density of 1 × 105/mL. Subsequently, 600μL of media containing 10 % FBS was added to the lower chambers, and the cells were incubated at 37°C for 36 h. To minimize potential bias in the results, three replicate wells were established for each experimental group, and each experiment was repeated thrice. The field of view for cell counting was randomly selected, and images were concurrently captured. Using Image J software, the number of migrating cells was quantified.
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