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Mouse anti hsc70 sc 7298

Manufactured by Santa Cruz Biotechnology
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Mouse anti-HSC70 (sc-7298) is a primary antibody that recognizes the heat shock cognate 70 kDa protein (HSC70). HSC70 is a constitutively expressed member of the heat shock protein 70 (HSP70) family and plays a role in the folding and transport of proteins. This antibody can be used to detect and study the expression of HSC70 in various applications.

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Lab products found in correlation

Lapatinib, by Selleck Chemicals (1 mentions) U73122, by Merck Group (1 mentions) Tak 632, by Selleck Chemicals (1 mentions) Aktviii, by Merck Group (1 mentions) Gf109203x, by Selleck Chemicals (1 mentions) Fr180204, by Selleck Chemicals (1 mentions) Phorbol myristate acetate (pma), by Selleck Chemicals (1 mentions) Ruxolitinib, by Selleck Chemicals (1 mentions) Zstk474, by Selleck Chemicals (1 mentions) Horseradish peroxidase labeled secondary antibody, by Agilent Technologies (1 mentions) Goat anti human vegf r3, by R&D Systems (1 mentions) Immobilon p pvdf, by Merck Group (1 mentions) Af349, by R&D Systems (1 mentions) Mini protean tgx precast gel, by Bio-Rad (1 mentions) Goat anti rabbit p0448, by Agilent Technologies (1 mentions) Goat anti mouse p0447, by Agilent Technologies (1 mentions) Immobilon western chemiluminescent hrp substrate, by Merck Group (1 mentions) Las 3000 imager, by Fujifilm (1 mentions) Gtx100118, by GeneTex (1 mentions) Affinipure goat anti rabbit igg, by Jackson ImmunoResearch (1 mentions)

Spelling variants of this product

HSC70 (90 citations) Anti-HSC70 (47 citations) Sc-7298 (28 citations) Mouse anti-HSC70 (24 citations) HSC70 (sc-7298) (19 citations) Anti-HSC70 (sc-7298; (4 citations) Mouse HSC70 (3 citations) Monoclonal mouse anti-HSC70 antibody (sc-7298) (2 citations)

5 protocols using mouse anti hsc70 sc 7298

1

Signaling Pathway Protein Detection

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Rabbit anti-PHLDA1 (ab133654) was from Abcam (Cambridge, UK). Mouse anti-HSC70 (sc-7298) was from Santa Cruz (Heidelberg, Germany). Mouse anti-AKT (9220S), mouse anti-ERK1/2 (4696S), rabbit anti-p-AKT (Ser473) (9271S), rabbit anti-p-ERK (Thr202/Tyr204) (9101S), rabbit anti-acetyl-histone H3 (Lys27) (8173P), rabbit anti-histone H3 (9715), rabbit p-STAT3 (Tyr705) (9145S), and mouse anti-STAT3 (9139) were from Cell Signalling Technology (Leiden, Netherlands). Lapatinib, ZSTK474, GF109203X, TAK632, ruxolitinib, FR180204, PMA, and 4SC-202 were purchased from Selleckchem (Houston, TX, USA). U73122 and AKT-VIII were purchased from Sigma.
Clayton N.S., Carter E.P., Fearon A.E., Heward J.A., Rodríguez Fernández L., Boughetane L., Wilkes E.H., Cutillas P.R, & Grose R.P. (2023). HDAC Inhibition Restores Response to HER2-Targeted Therapy in Breast Cancer via PHLDA1 Induction. International Journal of Molecular Sciences, 24(7), 6228.
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2

Quantification of VEGFR2 and VEGFR3 Expressions

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Lysates containing equal amounts of protein from lungs were separated in 7.5% Mini-PROTEAN TGX Precast gels (BIORAD). After blotting to polyvinylidene fluoride membranes (Immobilon-P PVDF; Millipore), the proteins were detected using goat anti-mouse VEGFR2 (AF644, 1:1000; R&D Systems), goat anti-mouse VEGFR3 (AF743, 1:1000; R&D Systems), mouse anti-HSC-70 (SC-7298, 1:5000; Santa Cruz Biotechnology), rabbit anti-human pVEGFR2 Tyr1175 (19A10, 1:1000; Cell Signaling), goat anti-human VEGFR2 (AF357, 1:500; R&D Systems), goat anti-human VEGFR3 (AF349, 1:1000; R&D Systems), or mouse anti-human VEGFR3 (9D9,25 (link) 1:1000)–specific primary antibodies. The blots were then probed with horseradish peroxidase–labeled secondary antibodies (Dako, Glostrup, Denmark), and the signal was visualized with the SuperSignal West Pico Chemiluminescent Substrate or the SuperSignal West Femto Maximum Sensitivity Substrate (Pierce, Rockford, IL).
Heinolainen K., Karaman S., D’Amico G., Tammela T., Sormunen R., Eklund L., Alitalo K, & Zarkada G. (2017). VEGFR3 Modulates Vascular Permeability by Controlling VEGF/VEGFR2 Signaling. Circulation research, 120(9), 1414-1425.
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3

Western Blot Analysis of CYP1A Enzymes

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Extracts from the HepaRG cells were separated using SDS-PAGE. The proteins were transferred to nitrocellulose membranes in a transfer buffer (25 mM Tris, 200 mM glycine, and ethanol 20%). The membranes were blocked in 5% low fat milk in Tris-buffer saline (TBS) (65 mM Tris pH 7.4, 150 mM NaCl) for 1 h at room temperature. The primary antibodies were purchased from Abcam (Cambridge, UK): mouse monoclonal anti-CYP1A2 S19 (ab22717) and rabbit polyclonal anti-CYP1A1 (ab3568), and from Santa Cruz Biotechnology (Dallas, TX, USA): mouse anti-HSC70 (SC-7298). The secondary antibodies were purchased from Dako (Santa Clara, CA, USA): goat anti-mouse (P0447) and goat anti-rabbit (P0448). The membranes were incubated with primary antibodies (1/1000) overnight at 4 °C. After being washed with TBS, appropriate secondary antibodies (1/1000) linked to horseradish peroxidase were incubated for one hour in 5% low-fat milk in TBS at room temperature. The immunocomplexes were visualized with an Immobilon Western Chemiluminescent HRP substrate (Millipore, Billerica, MA, USA), and scanned with a Fujifilm LAS-3000 imager (Fujifilm, Tokyo, Japan).
Alarcan J., Dubreil E., Huguet A., Hurtaud-Pessel D., Hessel-Pras S., Lampen A., Fessard V, & Le Hegarat L. (2017). Metabolism of the Marine Phycotoxin PTX-2 and Its Effects on Hepatic Xenobiotic Metabolism: Activation of Nuclear Receptors and Modulation of the Phase I Cytochrome P450. Toxins, 9(7), 212.
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4

Hepatitis B Virus Protein Detection Protocol

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The following primary antibodies were used in this study: rabbit anti-PKM2 (CST 4053, Cell Signaling Technology), rabbit anti-HBsAg (ad/ay, PAB13969, Abnova), normal mouse IgG (sc-2025, Santa Cruz Biotechnology), normal rabbit IgG (sc-2027, Santa Cruz Biotechnology), rabbit anti-HBcAg (B0586, DAKO), mouse anti-HA-tag (sc-7392, Santa Cruz Biotechnology), mouse anti-HSC70 (sc-7298, Santa Cruz Biotechnology), rabbit anti-SNAP-tag (P9310, New England BioLabs), rabbit anti-beta-tubulin (NB600-936, Novus Biologicals), mouse anti-beta-actin (NB600-501, Novus Biologicals), rabbit anti-GAPDH (GTX100118, GeneTex), rabbit anti-Grp78 (GTX113340, GeneTex), rabbit anti-PLK1 (GTX104302, GeneTex), rabbit anti-MAD2L1 (GTX104680, GeneTex), rabbit anti-Bcl-2 (GTX100064, GeneTex), and rabbit anti-PCNA (GTX100539, GeneTex). Mouse anti-LHBS/PreS1 (7H11), mouse anti-SHBS (86H6), and mouse anti-HBx (20F3) were kindly provided by Professor Ning-Shao Xia (Xiamen University, China) [33 (link)]. The following secondary antibodies were used in this study: peroxidase-conjugated AffiniPure goat anti-rabbit IgG (H+L, 111-035-003, Jackson ImmunoResearch), peroxidase-conjugated AffiniPure goat anti-mouse IgG (H+L, 115-035-003, Jackson ImmunoResearch), Alexa Fluor 488 donkey anti-rabbit IgG (H+L, A21206, Invitrogen) and Alexa Fluor 647 donkey anti-mouse IgG (H+L, A31571, Invitrogen).
Wu Y.H., Yang Y., Chen C.H., Hsiao C.J., Li T.N., Liao K.J., Watashi K., Chen B.S, & Wang L.H. (2021). Aerobic glycolysis supports hepatitis B virus protein synthesis through interaction between viral surface antigen and pyruvate kinase isoform M2. PLoS Pathogens, 17(3), e1008866.
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5

Western Blot Protein Detection

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Western blot analysis was performed, as described previously. 20 In brief, total protein (20 μg) or serum (0. After washing with PBST, membranes were incubated with HRP-conjugated streptavidin (Thermo Fisher Scientific Inc., Waltham, MA, USA) diluted 1:6,000 at room temperature for 60 min. Internal controls were mouse anti-Hsc70 (sc-7298; Santa Cruz Biotechnology, Santa Cruz, CA, USA) and diluted HRP-conjugated sheep anti-mouse IgG (GE Healthcare Life Science) antibodies (both diluted 1:2,000), which served as primary and secondary antibodies, respectively. Hsc70 was used for normalization.
Tian Z., Miyata K., Morinaga J., Horiguchi H., Kadomatsu T., Endo M., Zhao J., Zhu S., Sugizaki T., Sato M., Terada K., Okumura T., Murohara T, & Oike Y. (2018). Circulating ANGPTL2 Levels Increase in Humans and Mice Exhibiting Cardiac Dysfunction. Circulation journal : official journal of the Japanese Circulation Society, 82(2).
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