Mobile phases A (2% acetonitrile, adjusted pH to 10.0 using ammonium hydroxide) and B (98% acetonitrile) were used to develop a gradient elution. The lyophilized powder was dissolved in solution A and centrifuged at 12,000 × g for 10 minutes at room temperature. The sample was fractionated using a C18 column (Waters, Milford, United States of America BEH C18, 4.6 × 250 mm, 5 μm) on a Rigol, Portland, United States of America L3000 HPLC system, and the column oven was set as 45°C. The detail of elution gradient is shown in Supplementary Table S1 . The eluates were monitored at UV 214 nm, collected in a tube per minute, and combined into 10 fractions finally. All fractions were dried under vacuum and then reconstituted in 0.1% (v/v) formic acid in water.
Beh c18
Manufactured by Rigol
The BEH C18 is a high-performance liquid chromatography (HPLC) column from Rigol. It is designed for the separation and analysis of a wide range of organic compounds. The column features a bridged ethylene hybrid (BEH) particle technology that provides high efficiency and excellent peak shape.
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Lab products found in correlation
3 protocols using beh c18
1
Analytical Characterization of Lyophilized Powder
2
HPLC Fractionation of Lyophilized Powder
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Mobile phase A (2% acetonitrile, adjusted pH to 10.0 using ammonium hydroxide) and B (98% acetonitrile) were used to develop a gradient elution as shown in Table 2 . The lyophilized powder was dissolved in solution A and centrifuged at 12,000× g for 10 min at room temperature. The sample was fractionated using a C18 column (Waters BEH C18, 4.6 × 250 mm, 5 μm) on a Rigol L3000 HPLC system and the column oven was set as 45 °C. The eluates were monitored at UV 214 nm, collected for a tube per minute and combined into 10 fractions finally. All fractions were dried under vacuum and then reconstituted in 0.1% (v/v) formic acid in water.
3
HPLC Fractionation of Lyophilized Powder
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mobile phases A (2% acetonitrile, adjusted pH to 10.0 using ammonium hydroxide) and B (98% acetonitrile, adjusted pH to 10.0 using ammonium hydroxide) were used to develop a gradient elution. The lyophilized powder was dissolved in solution A and centrifuged at 12,000 g for 10 min at room temperature. The sample was fractionated using a C18 column (Waters BEH C18, 4.6 × 250 mm, 5 μm) on a Rigol L3000 HPLC system, and the column oven was set as 45°C. The detail of elution gradient is shown in Supplementary Table S1 . The eluates were monitored at UV 214nm, collected in a tube per minute, and combined into 10 fractions finally. All fractions were dried under vacuum and then reconstituted in 0.1% (v/v) formic acid (FA) in water.
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