Coomassie brilliant blue r 250

Luria–Bertani (LB) broth, agar, catechol, veratryl alcohol, magnesium sulfate, zinc sulfate, copper sulfate, ammonium sulfate, silver chloride, mercuric chloride, calcium chloride, sodium chloride, dipotassium hydrogen phosphate, potassium dihydrogen phosphate, silver nitrate, sodium nitrate, potassium nitrate, ammonium nitrate and sodium thiosulfate, sulfanilamide, N-(1-naphthyl)ethylenediamine dihydrochloride (NEDD), 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), Diethyl aminoethyl (DEAE)-cellulose and Biogel P-150 were procured from Himedia (Mumbai, India). Coomassie brilliant blue R-250, hydrogen peroxide and n-propanol were obtained from Loba Chemie (Mumbai, India), SD Fine (Mumbai, India) and Qualigens (Mumbai, India), respectively. Ammonium sulphate, NADH, L-DOPA, guaiacol, hydroxyquinolone and tartaric acid were supplied by SRL Pvt Ltd (Mumbai, India).

SIPC was generously provided by SumaSach’a (Mosquera, Cundinamarca, Colombia). BioLegend Inc. provided the protein ladder Prime-Step (San Diego, CA, USA). ITW Reagents PanReac-AppliChem (Darmstadt, Germany) supplied acrylamide, butanol, hydrochloric acid, mercaptoethanol, methanol, and tetramethylethylenediamine (TEMED). Loba-Chemie (Tarapur, India) provided bis-acrylamide, bromophenol blue, Coomassie Brilliant Blue R-250, glacial acetic acid, glycerin, glycine, persulfate ammonium (APS), SDS, and sodium hydroxide. Merck KGaA (Darmstadt, Germany) supplied the tris-hydroxymethyl aminomethane (TRIS).